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Petri dishes

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Petri dishes are circular, shallow, and transparent containers made of glass or plastic. They are commonly used in microbiology and cell culture laboratories to provide a sterile environment for growing and culturing microorganisms, cells, or tissues.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Agar, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Calcein green, by Merck Group (2 mentions) Bhi broth, by Thermo Fisher Scientific (2 mentions) Silica si beads, by Thermo Fisher Scientific (2 mentions) Tryptic soy broth (tsb), by Merck Group (2 mentions) Lsm 510, by Zeiss (2 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (2 mentions) Imaris 4, by Oxford Instruments (2 mentions) Jsm 6610, by JEOL (2 mentions) Pen strep, by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Hepes buffer, by Merck Group (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Rifampin, by Thermo Fisher Scientific (1 mentions)

69 protocols using petri dishes

1

Preclinical Evaluation of Bone Tissue Engineering

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Surgical supplies, reagents, Petri dishes, agar, and brain heart infusion (BHI) broth were purchased from Fisher Scientific (Waltham, MA). Fluids, analgesics, and anesthetics were provided by the University of Utah Comparative Medicine Center. Surgical tools and histological processing equipment/materials were provided by the Bone and Biofilm Research Lab (Salt Lake City, UT). V.A.C. Freedom® Therapy Units were purchased from KCI (San Antonio, TX). Martin® Tornado Air Cannon (Model BB4-12-28, Martin Engineering, Neponset, IL) was donated by Martin Engineering. Calcein green was purchased from Sigma Aldrich (St. Louis, MO). Silica (Si) beads were purchased from Fisher Scientific (Waltham, MA). Scanning electron microscopy (SEM) was performed on a JEOL JSM-6610 (Peabody, MA). Sanderson's Rapid Bone Stain (SRBS) was purchased from Dorn & Hart Microedge Inc. (Loxley, AL).
Epperson R.T., Isaacson B.M., Rothberg D.L., Olsen R.E., Kawaguchi B., Maxwell J.M., Dickerson M., Pasquina P.F., Shero J, & Williams D.L. (2021). Developing a combat-relevant translatable large animal model of heterotopic ossification. Bone Reports, 15, 101127.
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2

Antimicrobial Evaluation of Biofilms

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Tryptic soy broth (TSB) was purchased from MilliporeSigma (Burlington, MA), Petri dishes, agar, and general supplies/reagents from Fisher Scientific (Hampton, NH), and brain heart infusion (BHI) broth from Research Products International (Mt Prospect, IL). CDC biofilm reactors and titanium (Ti) coupons were purchased from Biosurface Technologies (Bozeman, MT). Levofloxacin was acquired from Chem-Impex International (Wood Dale, IL) and rifampin from ThermoFisher Scientific (Waltham, MA).
Ong J., Godfrey R., Nazarian A., Tam J., Isaacson B.M., Pasquina P.F, & Williams D.L. (2023). Comparison of Staphylococcus aureus tolerance between antimicrobial blue light, levofloxacin, and rifampin. Frontiers in Microbiology, 14, 1158558.
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3

Bone and Biofilm Research Protocols

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Surgical supplies, Petri dishes, agar, and brain heart infusion (BHI) broth were purchased from Fisher Scientific (Waltham, MA, USA). The University of Utah Comparative Medicine Center provided any fluids, analgesics, and anesthetics required. The Bone and Biofilm Research Lab. (Salt Lake City, UT, USA) provided surgical tools and histological processing equipment/materials. Martin Engineering donated the Martin® Tornado Air Cannon (Model BB4-12-28, Martin Engineering, Neponset, IL, USA). The V.A.C. Freedom® Therapy Units were purchased from KCI (San Antonio, TX, USA). Calcein green was purchased from Sigma Aldrich (St. Louis, MO, USA). Silica (Si) beads were purchased from Fisher Scientific (Waltham, MA, USA). Micro-CT scans were captured using a Quantum GX micro-CT (PerkinElmer; Waltham, MA, USA). Scanning electron microscopy was performed on a JEOL JSM-6610 (JEOL, Peabody, MA, USA). Sanderson’s Rapid Bone Stain (SRBS) was purchased from Dorn & Hart Microedge, Inc. (Loxley, AL, USA).
Epperson R.T., Isaacson B.M., Rothberg D.L., Olsen R.E., Kawaguchi B., Rasmussen R.M., Dickerson M., Pasquina P.F., Shero J, & Williams D.L. (2024). Determining Which Combinatorial Combat-Relevant Factors Contribute to Heterotopic Ossification Formation in an Ovine Model. Bioengineering, 11(4), 350.
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4

Differentiation of Embryonic Stem Cells

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ESCs were maintained on embryonic fibroblasts using standard conditions (Ernst et al., 2004b (link)). For in vitro differentiation, single-cell suspensions from dissociated ESC cultures were seeded at 10,000–20,000 cells/mL in Petri dishes (Fisher) with orbital rotation (50 rpm, Labnet Orbit 1000). The differentiation medium was Iscove's modified Dulbecco's medium (Mediatech) containing 15% fetal bovine serum (Gibco), 2 mM L-glutamine (Mediatech), 1% penicillin/streptomycin (Mediatech), 200 μg/mL holo bovine transferrin (Millipore), 4.5 × 10−4 M monothioglycerol (Sigma) and 50 μg/mL ascorbic acid (Sigma). Doxycycline (Enzo Life Sciences) was added to the differentiation medium at 1–2 μg/mL for the times indicated in each figure legend.
Yang W., Trahan G.D., Howell E.D., Speck N.A., Jones K.L., Gillen A.E., Riemondy K., Hesselberth J., Bryder D, & Ernst P. (2020). Enhancing Hematopoiesis from Murine Embryonic Stem Cells through MLL1-Induced Activation of a Rac/Rho/Integrin Signaling Axis. Stem Cell Reports, 14(2), 285-299.
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5

Insecticide Toxicity Assay for Cockroaches

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Technical insecticides were serially diluted with acetone and 0.5 μL of a dilution was applied to the ventral surface of the cockroach between the metacoxae with a repeating micro-pipette (Hamilton Company, Reno, NV, USA). At least 30 individuals were treated with each concentration. Following treatment, cockroaches were maintained in three groups of 10 in 10 cm diameter petri dishes (Fisher Scientific, Pittsburgh, PA) and provisioned with rat chow and water. Cockroaches topically treated with fipronil or indoxacarb were monitored for mortality daily for two days; those treated with hydramethylnon for five days. The greatest range of mortality for the AIs tested at given doses occurred at these two time points. Insects that could not right themselves within 30 seconds when flipped, and would exhibit erratic appendage movements were considered dead. Values for LD50 and LD90, and their respective fiducial limits, were estimated from Probit analysis in Polo Plus (LeOra Software Company, Petaluma, CA, USA).26
Ko A.E., Bieman D.N., Schal C, & Silverman J. (2016). Insecticide resistance and diminished secondary kill performance of bait formulations against German cockroaches (Dictyoptera: Blattellidae). Pest management science, 72(9), 1778-1784.
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6

Milk-Based Cell Culture Protocol

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Milk products including whole milk, 2% skim, and fat-free half-pint (236 ml) cartons were obtained from a local retail food market (Prairie Farms, Springfield, IL). A total of 11 boxes of each kind of milk, with expiration dates on and up to 10 days prior to the day of experiment, were used. Distilled water (1 gallon bottle) was obtained from Walmart (Bentonville, AR). Agarose (30 g bottle) was obtained from Biomedicals (Santa Ana, CA). Fetal bovine serum (FBS; 100 ml bottle) was obtained from HyClone (Logan, UT). Petri dishes (60 mm diameter), glass beaker, pH strips, hotplate stirrer and magnetic stir bar pipette, and pipette tips were obtained from Fisher Scientific (Pittsburgh, PA). Kitchen scale was obtained from Taylor (Oak Brooks, IL). The M150 biological microscope was obtained from Am Scope (Irvine, CA). The computer was obtained from Lenovo (Quarry Bay, Hong Kong), Image J software was obtained from NIH (Bethesda, Maryland), and Excel software was obtained from Microsoft (Redmond, Washington).
Yang E., Yang Q., Troemper B, & Zhang J. (2023). Investigation on Bacterial Growth and pH in Milk after the Expiration Date. The Scientific World Journal, 2023, 9982886.
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7

Seed Sterilization and Stratification

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To ensure maximum germination, the seeds from the SEN and NEB populations were sterilised in a 50:50 mix of sodium hypochlorite (Sigma) and water for 10 minutes. Then after, they were rinsed thoroughly with distilled water and placed on 0.4% plant agar (Duchefa) in 10 cm2 Petri- dishes (Fisher Scientific) and sealed with Parafilm (Sigma). The Petri-dishes were stored at 4°C for 21 days following which the seeds were dried on tissue paper overnight before sowing.
Kaundun S.S., Hutchings S.J., Dale R.P., Howell A., Morris J.A., Kramer V.C., Shivrain V.K, & Mcindoe E. (2017). Mechanism of resistance to mesotrione in an Amaranthus tuberculatus population from Nebraska, USA. PLoS ONE, 12(6), e0180095.
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8

Generation of hPSC-Derived Enteric Neural Crest Cells

Cited 2 times
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The generation of hPSC-derived ENCCs has been previously published (Bajpai et al., 2010 (link); Workman et al., 2017 (link)). Briefly for ENCC generation, confluent hPSCs were treated with collagenase IV (500 U/ml, Gibco) in mTeSR1 for 60–90 mins to detach colonies. Cells were diluted and washed with DMEM/F-12 (Gibco) and then gently triturated and resuspended in neural induction media, 1:1 ratio of DMEM/F12-GlutaMAX (Gibco) and Neurobasal Medium (Gibco) with B27 supplement (0.5x, Gibco), N2 supplement (0.5x, Gibco), pen-strep (1x, Gibco), insulin (5 μg/mL, Sigma-Aldrich), FGF2 (20 ng/mL, R&D Systems), and EGF (20 ng/mL, R&D Systems), on non-TC-treated petri dishes (6cm, Fisherbrand). Neural induction media was changed daily and all-trans RA (2 μM) was added on days 4 and 5 for posteriorization. Day 6 free-floating neurospheres were plated on human fibronectin (HFN, 3 μg/cm2, Corning) and fed neural induction media without RA for 4 days. Migrated cells were collected using a 90 sec Accutase treatment and passaged onto HFN. Passaged cells were allowed to grow to confluency for an additional 4 days and fed neural induction media without RA every day. Confluent cells were dissociated into single cells using Accutase (2–6 min), resuspended in 1–4mL DMEM/F-12 (Gibco), counted, and immediately combined with hAGOs, hFGOs, and hEOs (see below for recombination procedure).
Eicher A.K., Kechele D.O., Sundaram N., Berns H.M., Poling H.M., Haines L.E., Sanchez J.G., Kishimoto K., Krishnamurthy M., Han L., Zorn A.M., Helmrath M.A, & Wells J.M. (2021). Functional human gastrointestinal organoids can be engineered from three primary germ layers separately derived from pluripotent system cells. Cell stem cell, 29(1), 36-51.e6.
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9

Culturing Glioma Stem Cells and Cell Lines

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GSCs derived from high-grade glioma samples, the MES subtype (GSC-83, GSC-326) and the PN subtype (GSC-19, GSC-84), were described by us previously3 (link). The GSCs were cultured in suspension in GSC growth medium [DMEM-F12 (Cat# 10565, Life Technologies) supplemented with B27 (1:50), heparin (5 mg/mL), basic FGF (bFGF) (20 ng/mL), and EGF (20 ng/mL)] in 100 × 20 mm Petri dishes (Cat# 0875711Z, Fisher Scientific) at 37 °C with 5% CO2. Growth medium was changed every 3 or 4 days. U87MG cells (Cat# HTB-14, ATCC) were cultured in EMEM (Cat#11095080, Thermo Fisher Scientific) with 10% FBS(HI), Sodium Pyruvate (1 mM) (Cat#11360-070, Invitrogen), MEM supplemented with Non-Essential Amino acids (0.1 mM) (Cat#11140050, Thermo Fisher Scientific), antibiotic/antimycotic (4 ml) (Cat#15240062, Thermo Fisher Scientific) and Gentamycin (5 µg/ml) (Cat# 9354, Irvine Scientific). Growth medium was changed every 3 or 4 days. 293-FT cells (Cat# R70007, Thermo Fisher Scientific) were cultured in DMEM (Cat# 45000-304, VWR) with 10% FBS(HI), Glutamine (2 mM) (Cat# 25030081, Thermo Fisher Scientific) and antibiotic/antimycotic (4 ml) (Cat# 15240062, Thermo Fisher Scientific). All cells were cultured at 37 °C with 5% CO2. Details for each cell line used herein are in Supplementary Table 1.
Li J., Garavaglia S., Ye Z., Moretti A., Belyaeva O.V., Beiser A., Ibrahim M., Wilk A., McClellan S., Klyuyeva A.V., Goggans K.R., Kedishvili N.Y., Salter E.A., Wierzbicki A., Migaud M.E., Mullett S.J., Yates N.A., Camacho C.J., Rizzi M, & Sobol R.W. (2021). A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis in glioma stem cells. Communications Biology, 4, 1420.
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10

Oregano Essential Oil Antimicrobial Effects

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Tryptic soy broth (TSB) was purchased from MilliporeSigma (Burlington, MA, USA), Petri dishes, agar, and general supplies/reagents from Fisher Scientific (Hampton, NH, USA), brain heart infusion (BHI) broth from Research Products International (Mt Prospect, IL, USA), and Baird Parker (BP) agar was from Remel Incorporated (San Diego, CA, USA). Cell lifters were purchased from GenFollower Biotech (Shaoxing, China). CDC biofilm reactors were purchased from Biosurface Technologies (Bozeman, MT, USA). Stomacher bags were purchased from Seward Laboratory Systems Inc (Bohemia, NY, USA). Multiple bottles of oregano essential oil were purchased from PlantTherapy (Murray, UT, USA). Oil compositions were obtained from the company and the main ingredients are located in Table 1:
Ong J., Godfrey R., Isaacson B., Pasquina P, & Williams D. (2022). Determining the Antibiofilm Efficacy of Oregano Gel in an Ex Vivo Model of Percutaneous Osseointegrated Implants. Microorganisms, 10(11), 2133.
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