Mice were fed with either chow diet or Western-like diet beginning a week before injection of tumor cells. Blood (in EDTA) was collected from chow diet-fed or Western-like diet-fed mice 12 days after MB49 tumor inoculation. Plasma lipoprotein profiles were obtained by fast protein liquid chromatography (FPLC) as described previously60 (link),61 (link). Briefly, equal volumes of plasma from 5 mice per group were pooled and 200 μl of this pooled plasma was applied to a set of 2 Superose 6 (HR 10/30) columns linked in series. Lipoproteins were eluted by size exclusion into 0.5 ml fractions in EDTA/NaCl/NaN3 (1 mmol/L; 0.154 mol/L; 0.02%) at a flow rate of 0.5 mL/min. Cholesterol was measured in each fraction using an enzymatic cholesterol kit (Wako) according to manufacturers’ instructions.
Enzymatic cholesterol kit
Manufactured by Fujifilm
The Enzymatic Cholesterol Kit is a laboratory product designed to measure the total cholesterol levels in a sample. It utilizes an enzymatic method to quantify the amount of cholesterol present. The kit provides the necessary reagents and protocols to perform this analysis.
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Lab products found in correlation
2 protocols using enzymatic cholesterol kit
1
Plasma Lipoprotein Profile Analysis in Tumor-Bearing Mice
2
Plasma Lipoprotein Profile Analysis in Tumor-Bearing Mice
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Mice were fed with either chow diet or Western-like diet beginning a week before injection of tumor cells. Blood (in EDTA) was collected from chow diet-fed or Western-like diet-fed mice 12 days after MB49 tumor inoculation. Plasma lipoprotein profiles were obtained by fast protein liquid chromatography (FPLC) as described previously60 (link),61 (link). Briefly, equal volumes of plasma from 5 mice per group were pooled and 200 μl of this pooled plasma was applied to a set of 2 Superose 6 (HR 10/30) columns linked in series. Lipoproteins were eluted by size exclusion into 0.5 ml fractions in EDTA/NaCl/NaN3 (1 mmol/L; 0.154 mol/L; 0.02%) at a flow rate of 0.5 mL/min. Cholesterol was measured in each fraction using an enzymatic cholesterol kit (Wako) according to manufacturers’ instructions.
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