Anti lc3a b d3u4c

The primary antibodies used in this study were as follows: anti-BRG1 (EPNCIR111A) Abcam Cat# ab110641 (1:10,000); anti-Caspase3 (8G10), Cell Signaling Technology, Cat# 9665 (1:1000); anti-C-Caspase 3, Cell Signaling Technology, Cat# 9661 (1:1000); anti-PARP (46D11), Cell Signaling Technology, Cat# 9532 (1:1000); anti-Ki67 (B56), BD Biosciences, Cat# 550609 (1:500); anti-EpCAM (G8.8), BD Biosciences, Cat# 552370 (1:500); anti-ZO1, Invitrogen, Cat# 40-2200 (1:500); anti-8-OHd G, Abcam, Cat# ab48508 (1:500); anti-LC3A/B (D3U4C), Cell Signaling Technology, Cat# 12741 (1:1000); anti-ATG16L1 (D6A5), Cell Signaling Technology, Cat# 8089 (1:1000); anti-PCNA, Santa Cruz Biotechnology, Cat# SC-7909 (1:1000); anti-P-H3, Cell Signaling Technology, Cat# 9701 (1:1000); anti-E-Cadherin, Cell Signaling Technology, Cat# 3195T (1:1000); anti-DCLK1, Abcam, Cat# ab31704 (1:500); anti-Claudin1, Cell Signaling Technology, Cat# 4933T (1:1000); anti-ChgA, Abcam, Cat# ab715 (1:1000); anti-Hes1, Abcam, Cat# ab71559 (1:500). Raw data of immunoblotting can be found in the source data file.

Unless otherwise stated whole cell extracts were prepared by lysing cells directly in SDS sample buffer. SDS-PAGE was performed on 12.5% acrylamide gels. For Western blotting 0.2 μm nitrocellulose membranes were used. For blocking, the membranes were incubated in 5% fat-free milk powder and 0.1% Tween-20 in PBS. Antibodies and their sources were: anti-ubiquitin (Z0458, DAKO), anti-Rpt6 (p45-110, Enzo Biosciences), anti-20S proteasome α-subunits (MCP231, Enzo Biosciences), anti-FLCN (D14G9, Cell Signaling Technology), anti-GFP (3H9, Chromotek), anti-myc (9E1, Chromotek) anti-HA (12CA5, Roche), anti-Flag (M2, Sigma), anti-RGSHis (34650, Qiagen), anti-β-actin (AC74, Sigma), anti-NaK-ATPase (C464.6, Sigma), anti-LC3A/B (D3U4C, Cell Signaling Technology), anti-GAPDH (14C10, Cell Signaling Technology), anti-α tubulin (TAT-1, 00020911 Sigma). All secondary antibodies were purchased from DAKO. The Un-Scan-It v6.1 software (Silk Scientific) was used for densitometry.