2D migration and invasion assay were performed in transwell Boyden chambers (8.0 µm, Merck Millipore Ltd., Germany). Boyden chambers were precoated with Matrigel for invasion assay (1 mg/mL, Corning, Germany) and were left untreated for migration assay. After serum-free starvation for 16 h, cells were seeded at 2 × 105 cells in 300 µl serum-free medium into the upper inserts, while lower chambers contained different treatments. After 24 h of migration or invasion, cells were stained with crystal violet for 20 min. After swiping off cells attached on the top of the insert, migrated and invaded cells in different groups were imaged with a light microscope (Olympus BX43). Quantification of migrated and invaded cells was performed with the QCM™ 24-Well Colorimetric Cell Migration Assay Kit (Merck Millipore Ltd., Germany) via a colorimeter (VersaMax Microplate Reader, Molecular Devices, San Jose, CA, USA).
Qcm 24 well colorimetric cell migration assay kit
Manufactured by Merck Group
Sourced in Germany, United States
The QCM™ 24-Well Colorimetric Cell Migration Assay kit is a laboratory equipment product that measures cell migration. It provides a 24-well format for simultaneous analysis of multiple samples.
Automatically generated - may contain errors
5 protocols using qcm 24 well colorimetric cell migration assay kit
1
Quantitative Cell Migration and Invasion Assay
2
Cell Migration and Invasion Assay
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Migration and invasion assays were conducted as described [14 ] in transwell chambers (8.0 µm, Merck Millipore Ltd., Germany) without or with Matrigel coating (1 mg/ml, Corning, Germany), respectively. Cells at density of 2.5 × 105 in 300 µl serum-free medium were seeded into upper inserts and different treatment media were placed in the lower chamber. After 24 h, migrated and invaded cells were quantified with the QCM™ 24-Well Colorimetric Cell Migration Assay Kit (Merck Millipore Ltd., Germany) in a colorimeter (VersaMax Microplate Reader, Molecular Devices, San Jose, CA, USA).
3
Quantifying Cell Migration Dynamics
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Cell motility was examined by using QCM™ 24-Well Colorimetric Cell Migration Assay kit (Millipore). The siRNA-transfected Chang liver cells were harvested and then transferred into the 24-well plate inserted with trans-well. Cellular migration was determined in accordance with the manufacture’s protocol.
4
Transwell Migration Assay for Cell Lines
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Transwell migration assay was examined by using QCM™ 24-Well Colorimetric Cell Migration Assay kit (Millipore, USA). Cell lines including Chang liver cells, PLC/PRF/5 hepatoma cells, and HepG2 hepatoma cells (5 × 104 cells) were harvested and then transferred into the upper chamber of 24-well plate inserted with trans-well. The lower chamber was filled with culture medium containing fetal bovine serum. After incubation for 4 hours, cells in the upper chamber were carefully removed with a cotton swab. Cells that passed through the membrane to the lower chamber surface were fixed with 3.7% formaldehyde (in PBS) in for 20 minutes, then treated with 0.1% Triton X-100 (in PBS) for 1 minutes, and finally stained with 1:1000 DAPI (in PBS). Cells from each well were counted in 9 random high-power fields under a microscope (ZEISS Axiovert 200M, German). Each experiment was repeated in triplicate.
5
Quantitative Cell Migration Assay
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Cell migration was assayed using a QCM™ 24-well colorimetric cell migration assay kit (Millipore Corporation, Billerica, MA, USA) following the manufacturer's instructions. Cells were stimulated with 0.1 μM NNK in 10% FBS added to the lower chamber. After 16 h, non-migratedcells on the upper side of the filter were removed with a cotton swab; cells on the underside of the filter were stained with a cell stain solution, then subsequently extracted and detected on a standard microplate reader (at 560 nm wavelength).
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