Anti mtp

Anti-AMPKα, anti-phosphor-AMPKα (Thr172), anti-ACC, anti-phosphor-ACC (Ser 79) and anti-SCD1 antibodies were purchased from Cell Signaling Technology, Inc. Anti-FAS, anti-MTP, anti-PPARα and anti-LXRα antibodies were obtained from Abcam (Cambridge, MA, USA). T0901317 and GW9508 were purchased from Cayman Chemical (Ann Arbor, MI). STO-609 was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Lipofectamine 2000 reagent was purchased from Life Technologies (Carlsbad, CA,USA). Staining reagent of Oil Red O was purchased from Sigma (St. Louis, MO, USA). The triglyceride assay kit was obtained from Bioassay Systems (Hayward, CA, USA). The anti-GLT8D2 antibody was purchased from Santa Cruz(Santa Cruz, CA, USA). The anti-SREBP1 (sterol regulatory element-binding protein-1c) antibody, anti-MTP (microsomal triglyceride transfer protein) antibody, anti-CPT-1 (carnitine palmitoyltransferase-1) antibody and anti-SCD antibody were from Abcam (Beijing, China). Antibodies against His and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were from Marine Biological Laboratory (Beijing, China). Chemiluminescence kit for signal detection of Western blot is the product of Thermo Fisher (Boston, MA, USA). All other reagents were of analytical grade.