Total 180 gastric tissues including 100 primary carcinomas, 4 adenomas, 6 hamartomas, 6 hyperplastic polyps, and 64 normal gastric tissues were obtained were obtained from 100 gastric cancer patients and 80 noncancer patients by surgical resection in the Kyung Hee University Medical Center (Seoul, Korea). Signed informed consent was obtained from each patient. Tissue specimens were snap-frozen in liquid N2 and stored at −70 °C until used. Tissue slices were subjected to histopathological review and tumor specimens composed of at least 70% carcinoma cells and adjacent tissues found not to contain tumor cells were chosen for molecular analysis. Fourteen human gastric cancer cell lines (SNU5, SNU16, SNU216, SNU484, SNU601, SNU620, SNU638, SNU719, MKN1, MKN28, MKN45, MKN74, AGS, and KATO-III) were obtained from Korea Cell Line Bank (Seoul, Korea) or American Type Culture Collection (Rockville, MD).
Snu 5
Manufactured by Korean Cell Line Bank
Sourced in United States
The SNU-5 is a cell culture incubator designed for the maintenance and growth of cell lines. It features a temperature-controlled environment and precise temperature and CO2 regulation to ensure optimal conditions for cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Snu 638, by Korean Cell Line Bank (11 mentions)
Snu 16, by Korean Cell Line Bank (10 mentions)
Snu 216, by Korean Cell Line Bank (10 mentions)
Snu 1, by Korean Cell Line Bank (10 mentions)
Mkn28, by Korean Cell Line Bank (9 mentions)
Snu620, by Korean Cell Line Bank (8 mentions)
Snu 601, by Korean Cell Line Bank (8 mentions)
Snu 484, by Korean Cell Line Bank (8 mentions)
Snu 719, by Korean Cell Line Bank (8 mentions)
Snu 668, by Korean Cell Line Bank (8 mentions)
Mkn45, by Korean Cell Line Bank (7 mentions)
Mkn74, by Korean Cell Line Bank (5 mentions)
Kato 3, by Korean Cell Line Bank (4 mentions)
Nci n87, by Korean Cell Line Bank (4 mentions)
Ycc 2, by Korean Cell Line Bank (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Hs746t, by Korean Cell Line Bank (1 mentions)
E myco valid mycoplasma pcr detection kit, by iNtRON Biotechnology (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
12 protocols using snu 5
1
Gastric Cancer Tissue Collection
2
Gastric and Lung Cancer Cell Lines
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Five gastric cancer cell lines (SNU5, SNU620, SNU638, Hs746T, and MKN45) were obtained from the Korean Cell Line Bank. Normal immortalized cell lines (HFE145) were obtained from Dr. Ashktorab (Howard University, Washington, DC, USA). Two non-small cell lung cancer (NSCLC) cell lines, EBC-1 and H1993, were purchased from the Japanese Research Resources Bank and the American Type Culture Collection, respectively. All cell lines were authenticated by short tandem repeat analyses in Korea Genome Information Institute and were checked for mycoplasma using the e-Myco VALID Mycoplasma PCR Detection Kit (#25245; iNtRon Biotechnology, Inc., Seongnam-si, Korea).
3
Gastric Cancer Cell Line Cultivation
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Twelve gastric cancer cell lines (six were established from male patients: NCI-N87, SNU-484, SNU-1, SNU-638, KATOⅢ, MKN-1; six were established from female patients: SNU-5, MKN-28, SNU-216, SNU-16, AGS, SNU-620) were obtained from Korean Cell Line Bank (KCLB; Seoul, Korea). All of the cells were cultured in RPMI 1640 (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 g/mL streptomycin; Gibco BRL).
4
Human Gastric Carcinoma Cell Lines
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Human gastric carcinoma cells AGS, KATO-III, MKN-1, MKN-28, MKN-45, MKN-74, N87, SNU-1, SNU-5, SNU-16, SNU-216, SNU-484, SNU-601, SNU-620, SNU-638, SNU-668, and SNU-719 were purchased from the Korean Cell Line Bank (Seoul, Korea). YCC-1, YCC2, YCC-3, and YCC-7 were kindly provided by Dr. Hyun Cheol Chung (Yonsei Cancer Center, Seoul, Korea). OCUM-2M was kindly provided by Dr. Masakazu Yashiro (Osaka City University, Osaka, Japan). YCC-1, YCC-2, YCC-3, and YCC-7 were maintained in Dulbecco’s modified Eagle’s medium (Gibco-BRL, Carlsbad, CA) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 U/ml streptomycin, and 2 mM glutamine. All other cell lines were cultured in RPMI-1640 medium (Gibco-BRL) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 U/ml streptomycin, and 2 mM glutamine. All cells were incubated in a humidified atmosphere contained 5% CO2 at 37°C.
5
Culturing Human Gastric Cancer Cell Lines
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12 human gastric cancer cell lines (AGS, MKN28, YCC-2, KATOIII, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from Korea Cell Line Bank were cultured in DMEM or RPMI 1640 medium supplemented with 5% fetal bovine serum (FBS) and 1% Antibiotics as described previously [45 (link)]. The KCLB authenticate the phenotypes of these cell lines.
6
Gastric Cancer Cell Line Cultivation
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Twelve human gastric cancer cell lines (AGS, MKN28, YCC-2, KATOIII, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from the Korea Cell Line Bank (KCLB, Seoul, Korea) were cultured in RPMI-1640 medium supplemented with 5% fetal bovine serum (FBS) and 1% antibiotics. The phenotypes of these cell lines have been authenticated by the KCLB.
7
Culturing HEK293 and Stomach Cancer Cell Lines
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HEK293 (human embryonic kidney cell line) was cultured in DMEM (Gibco, Paisley, UK) containing 10% (v/v) heat‐inactivated FBS (WELGENE, Gyeongsangbuk‐do, Korea), 100 U/mL penicillin and 100 μg/mL streptomycin at 37°C in a humidified incubator containing 5% CO2. Stomach cancer cell lines, SNU1, SNU5, SNU620, SNU216, SNU484, SNU638, SNU668, MKN1, MKN28, MKN45, MKN74, and NCI‐N87, were obtained from the Korea Cell Line Bank (Seoul, Korea). HS746T and AGS cell line were obtained from the ATCC (Rockville, MD, USA). Stomach cancer cell lines were cultured in RPMI‐1640 medium (Gibco) containing 10% (v/v) heat‐inactivated FBS (WELGENE).
8
Gastric Cancer Cell Line Culture
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Cell lines and culture conditions AGS (American Tissue Type Culture Collection, USA), SNU1, SNU5, SNU16, SNU484, and NCI-N87 gastric adenocarcinoma cell lines (Korean Cell Line Bank), and Lenti-X cells were grown in RPMI (Corning, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% Penicillin-Streptomycin (P/S) (Gibco, USA). Eμ-Myc Cdkn2a Arf-/-leukemia cell line was grown in B-cell media (BCM) composed of DMEM and IMDM media supplemented with 10% FBS, 1% P/S and 0.1% 2-mercaptoethanol (Gibco, USA). The EGFR, mTOR, and c-Met mutation status data for gastric cancer cells were retrieved from the DepMap portal developed by Broad Institute [14, 15] .
9
Gastric Cancer Cell Line Culturing
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Twelve human gastric cancer cell lines (AGS, YCC-2, MKN28, KATO III, SNU-1, SNU-5, SNU-16, SNU-216, SNU-601, SNU-638, SNU-668, and SNU-719) obtained from the Korea Cell Line Bank (KCLB, Korea) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS: Corning Costar, USA) and 1% antibiotic-antimycotic (Gibco, USA). Cell cultures were maintained at 37°C in an atmosphere of 5% CO2. The phenotypes of these cell lines have been authenticated by the KCLB.
10
Gastric Cancer Cell Line Cultivation Protocol
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Total 18 gastric cancer cell lines (SNU-5, SNU-16, SNU-216, SNU-484, SNU-601, SNU-620, SNU-668, SNU-719, MKN-1, MKN-28, MKN-45, MKN-74, KATOIII, AGS, NCI-N87, SNU-1, SNU-520, and SNU-638), were purchased from the Korean Cell Line Bank. The cells were maintained under standard conditions (RPMI-1640 containing 25 mM HEPES, 10% fetal bovine serum, 100 unit/mL streptomycin, and 100 units/mL penicillin at 37 °C, 5% CO2). The 293 T cell line was cultured at 37 °C in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Medium and reagents for cell culture were purchased from WELGENE, Inc., Republic of Korea. Transfections were carried out using Fugene® HD (Promega) according to the manufacturer’s instructions.
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