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Sc 819

Manufactured by Santa Cruz Biotechnology
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Sc-819 is a laboratory instrument produced by Santa Cruz Biotechnology. It is designed for general laboratory use.

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Lab products found in correlation

Ab16667, by Abcam (1 mentions) Image pro plus software version 6, by Media Cybernetics (1 mentions) Mitochondria isolation kit, by Thermo Fisher Scientific (1 mentions) Sc 1703, by Santa Cruz Biotechnology (1 mentions) Immun blot pvdf membrane, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions) Protein a agarose bead, by Roche (1 mentions) Sodium orthovanadate, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail, by Santa Cruz Biotechnology (1 mentions) A3854, by Merck Group (1 mentions) Ripa lysis buffer system, by Santa Cruz Biotechnology (1 mentions) Pierce bicinchoninic acid bca protein assay, by Thermo Fisher Scientific (1 mentions) Anti c myc, by Abcam (1 mentions) Mini protean tgx gel, by Bio-Rad (1 mentions) Ab32072, by Abcam (1 mentions) Lung cancer tissue microarray, by Shanghai Outdo Biotech Co. (1 mentions) Ab32518, by Abcam (1 mentions) Ab24479, by Abcam (1 mentions) Ab32538, by Abcam (1 mentions) Sc 126, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-Mcl-1 (S-19) (sc-819) (2 citations) MCL-1 (sc-819, (17 citations) MCL-1 (sc-819) and NOXA (sc-56169) (2 citations) Anti-Mcl-1 (SC-819, (7 citations)

6 protocols using sc 819

1

Immunohistochemical Analysis of ESCC Tissues

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Tumor tissues obtained from ESCC patients or euthanized xenografted mice were embedded in paraffin and subjected to immunohistochemistry staining with specific antibodies against MCL-1 (1:100, sc-819, Santa Cruz Biotechnology) or Ki67 (1:200, ab16667, Abcam) according to the DAKO system protocol. Hematoxylin was used for counterstaining. Slides were viewed and photographed under a light microscope, and analyzed using Image-Pro Plus Software (version 6.2) program (Media Cybernetics).
Yu X., Li W., Xia Z., Xie L., Ma X., Liang Q., Liu L., Wang J., Zhou X., Yang Y, & Liu H. (2017). Targeting MCL-1 sensitizes human esophageal squamous cell carcinoma cells to cisplatin-induced apoptosis. BMC Cancer, 17, 449.
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2

Neutrophil Subcellular Protein Extraction

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Neutrophils were lysed in 1x Laemmli buffer containing 1∶100 (vol/vol) protease inhibitor cocktail set (Thermo Scientific, Nepean, Ontario, Canada). Nuclear and mitochondrial factions were prepared with the NE-PER Cytoplasmic Extraction kit and the Mitochondria Isolation kit respectively (both from Thermo Scientific). Proteins from 107 neutrophils, nuclear or mitochondrial factions were resolved by SDS-PAGE, transferred to Immun-Blot™-PVDF membrane (Bio-Rad Laboratories, Mississauga, Ontario, Canada), blocked with 5% nonfat milk, and probed with antibodies to Mcl-1 (rabbit polyclonal Ab, sc- 819, Santa Cruz Biotechnology, Santa Cruz, CA, USA), YY1 (rabbit polyclonal Ab, sc-1703, Santa Cruz Biotechnology), superoxide dismutase-2 (SOD-2, mouse mAb, clone A-2, Santa Cruz Biotechnology) or β-actin (Sigma-Aldrich) (5). Band density was quantified using the National Institutes of Health ImageJ software (http://rsb.info.nih.gov/ij/) and was expressed as a ratio of unstimulated cells following correction for loading discrepancies using the density of the actin, YY1 or SOD-2 band as appropriate.
El Kebir D., Damlaj A, & Filep J.G. (2014). Toll-Like Receptor 9 Signaling Delays Neutrophil Apoptosis by Increasing Transcription of Mcl-1. PLoS ONE, 9(1), e87006.
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3

Immunoprecipitation of Bim and Bcl-2

Cited 1 time
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Immunoprecipitation of Bim and Bcl-2 was performed as previously described [24 (link),26 ] using 2 μg of anti-Bim (2819, Cell Signaling Technology) or anti-Bcl-2 (SC-819, Santa Cruz Biotechnology, Santa Cruz, CA) antibody, 1 mg protein lysate, and Protein A agarose beads (Roche Diagnostics).
Su Y., Li X., Ma J., Zhao J., Liu S., Wang G., Edwards H., Taub J.W., Lin H, & Ge Y. (2017). Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo. Biochemical pharmacology, 148, 13-26.
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4

Protein Extraction and Western Blot Analysis

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For analysis of MYC (n = 7), MCL1 (n = 7) and RNA Pol II (n = 4), proteins were extracted with the RIPA lysis buffer system including phenylmethylsulfonyl fluoride, protease inhibitor cocktail and sodium orthovanadate (Santa Cruz Biotechnology, Dallas, TX, USA). Protein concentrations were measured using Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific, Waltham, MA, USA). Equal amounts of protein lysates were separated by SDS-PAGE (Mini-Protean TGX gels, gradient 8–16%, Bio Rad Laboratories, Hercules, CA, USA) and transferred to a nitrocellulose membrane.
The following primary antibodies were used: rabbit monoclonal anti-cMYC (Y69, dilution 1:4000; ab32072, Abcam, Cambridge, UK), rabbit polyclonal anti-MCL-1 (S19, dilution 1:4000; sc-819, SantaCruz Biotechnology, Dallas, TX, USA), RNAPol II C-terminal domain (CTD) rat monoclonal anti-Ser2-P (3E10), rat monoclonal anti-Ser5-P (3E8) and rat monoclonal anti-Ser7-P (4E12, dilution 1:20 each, ChromoTek, Martinsried, Germany) and mouse monoclonal anti-β-actin-HRP (AC-15, dilution 1:500000; A3854, Sigma-Aldrich, St. Louis, MO, USA). As secondary antibodies the anti-rabbit-IgG-donkey-HRP (711-036-152, Jackson ImmunoResearch, Cambridgeshire, UK) and the anti-rat-IgG-goat-HRP (sc-2006. Santa Cruz Biotechnology) were used.
Johansson P., Dierichs L., Klein-Hitpass L., Bergmann A.K., Möllmann M., Menninger S., Habenberger P., Klebl B., Siveke J.T., Dührsen U., Choidas A, & Dürig J. (2020). Anti-leukemic effect of CDK9 inhibition in T-cell prolymphocytic leukemia. Therapeutic Advances in Hematology, 11, 2040620720933761.
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5

Lung and Ovarian Cancer Tissue Microarray Analysis

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The lung cancer tissue microarray, which contained 30 cases of lung adenocarcinomas and their adjacent normal lung tissues, was purchased from Shanghai Outdo Biotech Company. The ovarian cancer specimens were collected during surgery in Ren Ji Hospital, Shanghai Jiao Tong University. The tissue slides were deparaffinized, treated with 3% H2O2 for 10 min, autoclaved in 10 mM citric sodium (pH 6.0) for 30 min to unmask antigens, rinsed in PBS, and then incubated with the primary antibodies against MCL1 (Santa Cruz, sc-819, 1:100) or USP13 (Santa Cruz, sc-514416, 1:250) at 4 °C overnight, followed by incubation with biotinylated secondary antibody for 1 hour at room temperature. Signal amplification and detection was performed using the DAB system according to the manufacturer’s instructions (Dako). Statistical significances of the correlation between USP13 and MCL1 expressions or the association of USP13 or MCL1 protein levels with tissue types (normal versus cancer) were determined using a χ2 (chi-squared) test. R represented the correlation coefficients.
Zhang S., Zhang M., Jing Y., Yin X., Ma P., Zhang Z., Wang X., Di W, & Zhuang G. (2018). Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nature Communications, 9, 215.
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6

Selinexor-Induced Tumor Regression Analysis

Cited 1 time
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KT-10 tumors that regress rapidly after treatment with selinexor, were harvested 24 hours after a single dose of drug (10 mg/kg). Other, less responsive tumors, were harvested 2 hours after dose 6 (MWF dosing) at 10 mg/kg/dose. Tumors were processed for immunoblotting as previously described [30 (link)]. Immunoblots were probed for p53, p21, PARP and cleaved PARP and XPO1/CRM1. Three control and three treated tumors were analyzed for each xenograft line. GAPDH was used as a loading control. KT-10, KT-11, SK-NEP-1, CHLA258, Rh28 and Rh30 tumors were fixed in 10% buffered formalin and paraffin sections were analyzed by H&E as well with the following antibodies: FOXO1 (#2880, Cell Signaling), IKB (ab32518, Abcam), NFKB (sc-8008, Santa Cruz), pRb-phos (ab76298, Abcam), Mcl-1 (sc-819, Santa Cruz), β-catenin (#610154 BD), ERK-phos (ab32538, Abcam), survivin (ab24479, Abcam), and p53 (sc-126, Santa Cruz).
Attiyeh E.F., Maris J.M., Lock R., Reynolds C.P., Kang M.H., Carol H., Gorlick R., Kolb E.A., Keir S.T., Wu J., Landesman Y., Shacham S., Lyalin D., Kurmasheva R.T., Houghton P.J, & Smith M.A. (2015). Pharmacodynamic and genomic markers associated with response to the XPO1/CRM1 inhibitor selinexor (KPT-330): a report from the Pediatric Preclinical Testing Program. Pediatric blood & cancer, 63(2), 276-286.
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