Oasis prime hlb spe cartridges

1,2-Phenanthrenequinone (1,2-PheQ) and 3,4-phenanthrenequinone (3,4-PheQ) were prepared as previously described (Figure 3) (Magdziak et al., 2002 (link)).) [¹³C₄]1,2-PheQ was similarly prepared from [¹³C₄]1-hydroxyphenanthrene ([¹³C₄]1-OHPhe), Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). 1-Hydroxyphenanthrene (1-OHPhe) and 4-hydroxyphenanthrene (4-OHPhe) were obtained from Toronto Research Chemicals (North York, ON, Canada). 9,10-Phenanthrenequinone (9,10-PheQ), benzaldehyde, formic acid, ammonium acetate and sodium acetate were purchased from Millipore Sigma (St. Louis, MO, USA). 8-iso-PGF_2α and [D₄]8-iso-PGF_2α were procured from Cayman Chemical (Ann Arbor, MI, USA). β-Glucuronidase and arylsulfatase (from Helix pomatia) were obtained from Roche Diagnostics Corp. (Indianapolis, IN, USA). Oasis® PRiME HLB SPE cartridges (200 mg/6 mL/# 186008057) were from Waters (Milford, MA, USA). A Bond Elut C18 96 square-well plate (100 mg/2 mL/# A396011C) was purchased from Agilent (Santa Clara, CA, USA). All solvents used in this study including hexane, isopropanol, acetonitrile, ethyl acetate and methanol were HPLC grade and obtained from Fisher Scientific (Fair Lawn, NJ, USA). Ultrapure H₂O from a Millipore Milli-Q Advantage A10 Water Purification System was used throughout the study.