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Milliplex map mouse metabolic hormone magnetic bead panel

Manufactured by Merck Group
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Sourced in United States, Germany

The Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel is a multiplex assay that allows for the simultaneous detection and quantification of multiple mouse metabolic hormones in a single sample. The panel utilizes magnetic bead technology and is designed for use with the Luminex or similar xMAP platform.

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Lab products found in correlation

Luminex magpix system, by Merck Group (3 mentions) Benedict s reagent, by HiMedia (2 mentions) Periodic acid schiff stain kit mucin stain no ab150680, by Abcam (2 mentions) Enzychrom ketone body assay kit ekbd 100, by BioAssay Systems (2 mentions) Triglyceride quantification kit, by Abcam (1 mentions) Mouse leptin elisa kit, by Crystal Chem (1 mentions) Dppiv, by Merck Group (1 mentions) Aprotinin, by Roche (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Contour blood glucose monitor, by Bayer (1 mentions) Mouse adiponectin elisa kit, by Abcam (1 mentions) Bio plex 5, by Bio-Rad (1 mentions)

Close spelling variants of this product

Milliplex Map Mouse Metabolic Hormone Magnetic Bead Panel- Metabolism Multiplex Assay MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (MMHMAG-44K; MMHMAG-44K Milliplex map mouse metabolic hormone magnetic bead panel – metabolism multiplex assay Mouse Metabolic Hormone Magnetic Bead Panel Milliplex Mouse Metabolic Magnetic Bead Panel Milliplex Mouse metabolic hormone panel MILLIPLEX MAP Milliplex panels Magnetic beads

13 protocols using milliplex map mouse metabolic hormone magnetic bead panel

1

Metabolic Hormone Profiling in Plasma

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A MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel was used to assess metabolic hormone levels in the plasma (Millipore, USA). The assay was performed according to the manufacturer’s protocol using undiluted plasma. The plate was run on a MAGPIX system with xPonent software (Luminex Corp, US).
Schofield S., Parkinson J., Henley A., Sahuri M., Sanchez-Canon G, & Bell J. (2016). Metabolic dysfunction following weight-cycling in male mice. International journal of obesity (2005), 41(3), 402-411.
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2

Metabolic Hormone Profiling in Rodents

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Plasma insulin and leptin levels were analysed by ELISA using the MILLIPLEX® MAP Mouse Metabolic Hormone Magnetic Bead Panel (Millipore, MMHMAG-44 K) accordingly to the manufacturer's instructions. Plasma ghrelin levels were analysed using the Rat/Mouse Ghrelin (Total) ELISA Kits (Millipore, EZGRA-88 K). Triglycerides levels were analysed with a Triglyceride Quantification Kit (Abcam Ltd, ab65336) following the to manufacturer's instructions. Corticosterone levels were assayed using ELISA kits (Enzo Life Sciences, ADI-900–097) according to the manufacturer's instructions.
Schellekens H., Torres-Fuentes C., van de Wouw M., Long-Smith C.M., Mitchell A., Strain C., Berding K., Bastiaanssen T.F., Rea K., Golubeva A.V., Arboleya S., Verpaalen M., Pusceddu M.M., Murphy A., Fouhy F., Murphy K., Ross P., Roy B.L., Stanton C., Dinan T.G, & Cryan J.F. (2020). Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human. EBioMedicine, 63, 103176.
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3

Plasma Leptin Levels in Genetically Modified Mice

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Plasma leptin levels were measured in GNX mice of both genotypes at 8 and 20 weeks. Blood samples were collected in ethylenediaminetertracetic acid (EDTA) and a protease inhibitor cocktail (Complete, Roche Applied Science, Mannheim, Germany), and the plasma immediately harvested and stored at −20 C. For the 8 week GNX mice, leptin concentrations were measured using the Milliplex Map Mouse Metabolic Hormone Magnetic Bead Panel (Millipore, Missouri, USA, cat no. MMHMAG-44K) using Luminex technology and Exponent software in accordance with manufacturer’s instructions. The minimum detectable concentration of leptin was 19 pg/mL and the intra-assay CV% was 5%. For 20 week GNX mice, leptin concentrations were determined with a mouse leptin ELISA kit (#90030; Crystal Chem Inc.) in accordance with the manufacturer’s instructions. The minimum detectable concentration of leptin was 2 pg/mL and the intra-assay CV% was <10%.
De Bond J.A., Tolson K.P., Nasamran C., Kauffman A.S, & Smith J.T. (2016). Unaltered hypothalamic metabolic gene expression in Kiss1r KO mice despite obesity and reduced energy expenditure. Journal of neuroendocrinology, 28(10), 10.1111/jne.12430.
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4

Plasma Hormone Analysis in Fasted and Fed Mice

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At the end of the EchoMRI experiments, blood plasma was obtained from VIP−/− (n=7) and WT (n=8) mice after overnight fasting (18:00-06:00 h) and in postprandial conditions consisting in overnight fasting (18:00-06:00 h) followed by re-feeding period (06:00-10:00 h), blood being collected 1 hour after feeding. The collected plasma were then added with a cocktail of protease inhibitors containing DPPIV (Millipore; Billerica, MA), Roche Complete Protease Inhibitor Cocktail (Roche), Aprotinin (Fisher Scientific), and Roche Pefabloc SC (AEBSF) (Roche) and stored frozen at −80C until assayed. Plasma levels of active ghrelin, glucagon-like peptide-1 (GLP-1), peptide YY (PYY), Insulin, Glucagon, and Leptin were analyzed in duplicate using the Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel (Millipore, Billerica, MA). Adiponectin was measured in duplicate by EIA (Millipore).
Vu J., Larauche M., Flores M., Luong L., Norris J., Oh S., Li-Jung L., Waschek J., Pisegna J, & Germano P. (2015). REGULATION OF APPETITE, BODY COMPOSITION AND METABOLIC HORMONES BY VASOACTIVE INTESTINAL POLYPEPTIDE (VIP). Journal of molecular neuroscience : MN, 56(2), 377-387.
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5

Metabolic Hormone Profiling in Plasma

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A MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel was used to assess metabolic hormone levels in the plasma (Millipore, USA). The assay was performed according to the manufacturer’s protocol using undiluted plasma. The plate was run on a MAGPIX system with xPonent software (Luminex Corp, US).
Schofield S., Parkinson J., Henley A., Sahuri M., Sanchez-Canon G, & Bell J. (2016). Metabolic dysfunction following weight-cycling in male mice. International journal of obesity (2005), 41(3), 402-411.
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6

Plasma Hormone Measurement Protocol

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Hormones were measured in plasma from cardiac puncture (CP) or from hepatic portal vein (HPV) (n = 4–8) collected with incorporation of Protease Inhibitor Cocktail for General Use (Sigma cat# P2714) using Millipore’s MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead panel (Merck Millipore, Feltham, UK).
Drew J.E., Reichardt N., Williams L.M., Mayer C.D., Walker A.W., Farquharson A.J., Kastora S., Farquharson F., Milligan G., Morrison D.J., Preston T., Flint H.J, & Louis P. (2018). Dietary fibers inhibit obesity in mice, but host responses in the cecum and liver appear unrelated to fiber-specific changes in cecal bacterial taxonomic composition. Scientific Reports, 8, 15566.
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7

Quantifying Metabolic Hormones in Serum

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The concentrations of amylin, insulin and leptin in serum were quantified using the MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (Merck Millipore) in accordance to the manufacturer’s instructions.
Nie T., Zhang S., Vazhoor Amarsingh G., Liu H., McCann M.J, & Cooper G.J. (2019). Altered metabolic gene expression in the brain of a triprolyl-human amylin transgenic mouse model of type 2 diabetes. Scientific Reports, 9, 14588.
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8

Metabolic Biomarkers in Mice

Cited 2 times
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Blood glucose, following a 6 h fast, was measured prior to start of diet, at diet switch, and at sacrifice following a 6 h fast using a Bayer Contour Blood Glucose Monitor (Bayer HealthCare LLC, Tarrytown, NY, USA). Metabolically relevant hormones including leptin, insulin, IL-6, MCP-1, and TNF-α were measured in the plasma collected at sacrifice using the Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel in the Luminex MAGPIX system (EMD Millipore, Billerica, MA, USA). The homeostasis model assessment was used to calculate the approximate insulin resistance (HOMAIR) using the formula (blood glucose (mg/dl at sacrifice) × plasma insulin levels (at sacrifice)/405) as previously described (14 (link), 28 (link)). Adiponectin concentrations in plasma collected at sacrifice were measured using the adiponectin mouse ELISA kit (ab108785; Abcam, Cambridge, MA, USA) following the manufacturer’s protocol. The leptin:adiponectin ratio was calculated using the measures obtained from the Luminex cytokine panel and adiponectin ELISA.
Sundaram S., Le T.L., Essaid L., Freemerman A.J., Huang M.J., Galanko J.A., McNaughton K.K., Bendt K.M., Darr D.B., Troester M.A, & Makowski L. (2014). Weight Loss Reversed Obesity-Induced HGF/c-Met Pathway and Basal-Like Breast Cancer Progression. Frontiers in Oncology, 4, 175.
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9

Metabolic Hormone and Inflammation Profiling

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Plasma collected at sacrifice was used for measuring metabolically relevant hormones and inflammatory mediators (insulin, IL-6, MCP-1 and TNF-α) using the Milliplex MAP Mouse Metabolic Hormone Magnetic Bead Panel in the Luminex MAGPIX system (EMD Millipore, Billerica, MA). The homeostasis model assessment-insulin resistance (HOMAIR) was used to calculate the approximate insulin resistance using the formula (glucose (mg/dl at sacrifice)×insulin (at sacrifice)/405) as previously described [21] , [36] . Estrogen and progesterone plasma concentrations were measured using ELISA assays following the manufacturer’s protocol (Novatein Bio; Cambridge, MA).
Sundaram S., Freemerman A.J., Galanko J.A., McNaughton K.K., Bendt K.M., Darr D.B., Troester M.A, & Makowski L. (2014). Obesity-Mediated Regulation of HGF/c-Met Is Associated with Reduced Basal-Like Breast Cancer Latency in Parous Mice. PLoS ONE, 9(10), e111394.
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10

Multiplex Hormone Quantification

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Plasma insulin, GLP-1, GIP and leptin were measured with the MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (Merck) according to the manufacturer's instructions. The assay was read with the Bio-Plex 200 multiplex suspension array system in combination with the Bio-Plex 5.0 Software (Bio-Rad, Hercules, CA, USA).
Hassan A.M., Mancano G., Kashofer K., Liebisch G., Farzi A., Zenz G., Claus S.P, & Holzer P. (2022). Anhedonia induced by high-fat diet in mice depends on gut microbiota and leptin. Nutritional neuroscience, 25(2).
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