Cells in the logarithmic growth phase were harvested and seeded into 96-well plates at a density of 5 × 103 cells/well with 180 μL of medium. After an overnight incubation at 37°C in 5% CO2, the cells were treated with apigenin (0, 0.1, 1, 10, 20, 50, or 100 μM) for 24, 48, and 72 h. Thereafter, 10 μL of CCK-8 solution (Bioswamp, Wuhan, China) was added to each well, and the plates were subsequently incubated at 37°C for 4 h. The absorbance of each well was measured using an AMR-100 apparatus (Allsheng, Hangzhou, China).
Amr 100 apparatus
Manufactured by Allsheng
Sourced in China
The AMR-100 apparatus is a laboratory instrument used for measuring the magnetic properties of materials. It is designed to perform alternating magnetoresistance (AMR) measurements, which is a technique used to study the electrical resistance of materials as a function of an applied magnetic field.
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Lab products found in correlation
2 protocols using amr 100 apparatus
1
Apigenin Cytotoxicity Assessment in Cells
2
Cell Proliferation Assay for Multiple Myeloma
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RPMI 8226 and U266 MM cells proliferation was determined using the cell counting kit-8 (CCK-8) assay. 3 × 10 5 harvested RPMI 8226 and U266 cells were seeded in 96-well plates, and maintained at 37 °C for 24, 48, and 72 h. Thereafter, cells were maintained for 4 h with 10 µL of CCK-8 solution (CA1210, Solarbio, Beijing, China). The optical density was detected at 450 nm using an AMR-100 apparatus (Allsheng, Hangzhou, China).
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