Proteins were extracted from SK-N-MC cells using SDS sample buffer and boiled at 99°C for 10 min prior to western blotting. Primary antibodies used were anti-NUDT16 (SAB2107004, 1:2,000) from Sigma-Aldrich; anti-p-ATR (2853, 1:1,000), anti-p-ATM (5883, 1:1,000), anti-p-CHK1 (2348, 1:1,000), anti-p-CHK2 (2197, 1:1,000), anti-cleaved caspase 3 (9664, 1:500), and anti-myc (2276, 1:2,000) from Cell Signaling Technology; anti-Dicer (ab14601, 1:2,000), anti-TDP-43 (ab109535, 1:2,000), and anti-β-tubulin (ab6046, 1:2,000) from Abcam; and anti-GFP (JL-8, 1:4,000) from Clontech. Secondary antibodies were goat anti-rabbit (11-035-045, 1:5,000) and goat anti-mouse (115-035-062, 1:5,000) from Jackson ImmunoResearch.
Goat anti mouse 115 035 062
Manufactured by Jackson ImmunoResearch
Sourced in United States
Goat anti-mouse (115-035-062) is a secondary antibody product manufactured by Jackson ImmunoResearch. It is designed to detect and bind to mouse primary antibodies, allowing for their visualization and detection in various immunological applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp jl 8, by Takara Bio (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti p chk1, by Cell Signaling Technology (1 mentions)
Anti myc, by Cell Signaling Technology (1 mentions)
Anti β tubulin ab6046, by Abcam (1 mentions)
Anti p chk2, by Cell Signaling Technology (1 mentions)
Anti p atm, by Cell Signaling Technology (1 mentions)
Anti dicer ab14601, by Abcam (1 mentions)
Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
2 protocols using goat anti mouse 115 035 062
1
Western Blotting of Signaling Proteins
2
Investigating EGFR and EMT Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Goat anti-rabbit (111-035-045) and goat anti-mouse (115-035-062) antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). Anti-β-actin (sc-47778) and anti-HBEGF (sc-74441) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-EGFR (ab52894) antibody was purchased from Abcam (Cambridge, United Kingdom), and the anti-Zeb2 (NBP1-82991) antibody was purchased from Novus Biologicals (Minneapolis, MN, USA). Anti-phospho-EGFR (Tyr1068) (#2234), (Tyr1173) (#4407), anti-E-cadherin (#14472), anti-Zeb1 (#3396), anti-Slug (#9585), anti-c-Jun (#9165), anti-phospho-c-Jun (#3270), anti-P65 (#8242), and anti-phospho-P65 (#3033) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Recombinant human HBEGF (cat. no. 100-47) was purchased from Peprotech (Rocky Hill, NJ, USA). The NF-κB inhibitor BAY 11-7082 (cat. no. B5556) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and the AP-1 inhibitor SR 11302 (cat. no. 2476) was purchased from Tocris Bioscience (Ellisville, MO, USA).
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