Live dead ir stain

Manufactured by Thermo Fisher Scientific

The Live Dead-IR stain is a fluorescent dye used to distinguish between live and dead cells in a sample. It provides a simple, reliable method for assessing cell viability.

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Lab products found in correlation

Alexa700 conjugated ifnγ, by BD (2 mentions) Brilliant violet 650 conjugated cd4, by BioLegend (1 mentions) Percp conjugated cd3, by BD (1 mentions) Cd3 clone sp34 2, by BD (1 mentions) Cd4 clone okt4, by BioLegend (1 mentions) Cd8 clone sk1, by BD (1 mentions)

Close spelling variants of this product

IR-LIVE/DEAD® Fixable Dead Cell Stains LIVE/DEAD™ Fixable Near-IR Dead Cell stain dye LIVE/DEAD Fixable Near-IR Dead Cell Stain reagent LIVE/DEAD Fixable Near-IR cell stain kit Live/Dead fixable near-IR stain LIVE-DEAD® Fixable yellow or near-IR dead cell stain kit LIVE/DEAD® fixable Near-IR stain kit LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit LIVE/DEAD fixable near-infrared (IR) dead cell stain kit LIVE/DEAD Near IR stain

2 protocols using live dead ir stain

Comprehensive Multiparametric Immune Profiling

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The following antibodies were used. FITC conjugated Bcl-2 (clone Bcl-2/100; BD Biosciences), PE-conjugated CXCR5 (clone MU5UBEE; eBioscience), PerCP conjugated CD3 (Clone SP-34-2; BD Biosciences), PeCy7 conjugated CD28 (Clone CD28.2; eBiosciences), PE-TR conjugated CD95 (clone; BD Biosciences), Brilliant Violet 421- conjugated CD279 (PD-1; Clone EH12.1; Biolegend), V500 conjugated CD8 (Clone SK1; BD Bioscience), APC conjugated CCR5 (Clone 3A9, BD Biosciences), Live Dead-IR stain (Invitrogen), Alexa700 conjugated Ki-67 (Clone B56; BD Biosciences), FITC conjugated Bcl-6 (Clone K112-91; BD Biosciences), Brilliant Violet 650 conjugated CD4 (Clone OKT4; Biolegend), FITC conjugated IL-17A (Clone eBio64Dec15; ebiosciences), PE conjugated IL-21 (Clone 3A3-N2; BD Biosciences), PerCP-conjugated CD4 (Clone L200; BD Biosciences), PeCy7 conjugated anti-CD279 (PD-1; clone EH12.1; Biolegend), PacBlue conjugated CD3 (Clone SP-34-2; BD Biosciences), APC conjugated IL-2 (Clone MQ1-17H12; BD Biosciences), Alexa700 conjugated IFNγ (Clone B27; BD Biosciences).

Mylvaganam G.H., Velu V., Hong J.J., Sadagopal S., Kwa S., Basu R., Lawson B., Villinger F, & Amara R.R. (2014). Diminished Viral Control during SIV Infection is Associated with Aberrant PD-1hi CD4 T cell Enrichment in the Lymphoid Follicles of the Rectal Mucosa. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4527-4536.

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Multiparameter Phenotyping of Immune Cells

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Cells were stained with fluorochrome-conjugated Abs specific for CXCR5 (clone MU5UBEE; eBioscience), CD3 (clone SP-34-2; BD Biosciences), CXCR3 (clone IC6; BD Biosciences), CCR6 (clone 11A9), CCR4 (clone 1G1), ICOS (clone C398.4A; ebioscience), CD95 (clone; BD Biosciences), PD-1(Clone EH12.1; Biolegend), CD8 (Clone SK1; BD Bioscience), CD4 (clone; OKT4; biolegend), CCR5 (Clone 3A9, BD Biosciences), α4β7 (Act1, NHP reagent resource), Live Dead-IR stain (Invitrogen), Ki-67 (Clone B56; BD Biosciences), Bcl-6 (clone K112-91), IL-21 (Clone 3A3-N2; BD Biosciences), CD4 (Clone L200; BD Biosciences), Alexa700 conjugated IFNγ (Clone B27; BD Biosciences). FITC conjugated CD40L (Clone TRAP1; BD Biosciences).

Velu V., Mylvaganam G.H., Gangadhara S., Hong J.J., Iyer S.S., Gumber S., Ibegbu C.C., Villinger F, & Amara R.R. (2016). Induction of Th1 biased Tfh (Tfh1 cells) in lymphoid tissues during chronic SIV infection defines functionally distinct germinal center Tfh cells. Journal of immunology (Baltimore, Md. : 1950), 197(5), 1832-1842.

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