All chemicals used in the present study were of either analytical or molecular biology grades and were obtained from commercial sources. Media components were purchased from Difco (USA). Oligonucleotides were purchased from Sigma (India) and IDT (USA). Restriction enzymes, Vent DNA polymerase, and other DNA-modifying enzymes were obtained from New England Biolabs (USA). Gel-extraction kits, plasmid miniprep columns, and the Ni-NTA agarose resin were obtained from Qiagen (Germany). Hybridization nitrocellulose membrane (filter type 0.45 μm) and Luminata forte Western horseradish peroxidase (HRP) substrate was obtained from Millipore (India). Anti-His mouse monoclonal antibody (27E8) and horse anti-mouse HRP-linked antibody were procured from Cell Signaling Technology (USA). Anti-GSH mouse monoclonal antibody (ab19534) was from Abcam (UK). Alexa Fluor 488–conjugated goat anti-mouse antibody was obtained from Molecular Probes (USA). Coelentrazine was purchased from Promega (USA).
Plasmid miniprep columns
Manufactured by Qiagen
Sourced in Germany
Plasmid miniprep columns are a laboratory equipment used for the purification of plasmid DNA from bacterial cultures. The core function of these columns is to isolate and extract plasmid DNA from samples, enabling further downstream applications such as DNA sequencing, cloning, and transfection experiments.
Automatically generated - may contain errors
Lab products found in correlation
Horse anti mouse hrp linked antibody, by Cell Signaling Technology (2 mentions)
Vent dna polymerase, by New England Biolabs (2 mentions)
Gel extraction kit, by Qiagen (2 mentions)
Restriction enzyme, by New England Biolabs (2 mentions)
Coelentrazine, by Promega (1 mentions)
Ni nta agarose resin, by Qiagen (1 mentions)
Alexa fluor 488 conjugated goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
Luminata forte western horseradish peroxidase hrp substrate, by Merck Group (1 mentions)
Oligonucleotide, by Merck Group (1 mentions)
Ecl plus western blotting detection reagent, by Cytiva (1 mentions)
Ha tag 6e2 mouse monoclonal antibody, by Cell Signaling Technology (1 mentions)
Alexa flour 488 conjugated goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions)
Hybond ecl nitrocellulose membrane, by Cytiva (1 mentions)
2 protocols using plasmid miniprep columns
1
Molecular Cloning and Protein Purification
2
Detailed Biochemical Experimental Protocols
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All the chemicals used in this study were analytical grade and obtained from commercial sources. Media components were purchased from Difco (Detroit, MI) Sigma Aldrich, (St. Louis, MO), HiMedia, (Mumbai, India), Merck India Ltd (Mumbai, India), and USB Corporation (Cleveland, OH). Oligonucleotides were purchased from Sigma India. Restriction enzymes, Vent DNA polymerase, and other DNA-modifying enzymes were obtained from New England Biolabs (Beverly, MA). DNA sequencing kit (ABI PRISM 310 XL with dye termination cycle sequencing ready reaction kit) was obtained from Perkin Elmer, (Norwalk, CT). Gel-extraction kits and plasmid miniprep columns were obtained from QIAGEN (Valencia, CA) or Sigma (St. Louis, MO). [35S] GSH (specific activity 1000 Ci mmol-1) was purchased from Bhabha Atomic Research Centre, Mumbai, India. HA-Tag (6E2) mouse monoclonal antibody and horse anti-mouse HRP-linked antibody were bought from Cell Signaling (Danvers, MA). Alexa Flour 488 conjugated goat anti-mouse antibody was obtained from Molecular Probes (Eugene, OR). Hybond ECL (nitrocellulose) membrane and ECL plus Western blotting detection reagents were purchased from Amersham Biosciences (UK).
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