The largest database of trusted experimental protocols

Bioanalyzer rna qc analysis

Manufactured by Agilent Technologies
Sourced in United States

The Bioanalyzer RNA QC analysis is a lab equipment product that provides automated analysis of RNA samples. It evaluates the integrity and quality of RNA samples using electrophoretic separation and laser-induced fluorescence detection.

Automatically generated - may contain errors

2 protocols using bioanalyzer rna qc analysis

1

Laser-Capture Microdissection of Purkinje Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laser-capture microdissection and mRNA isolation were performed as previously reported (Du et al., 2019 (link)). Frozen cerebellar sections (10 μm thick; ∼P100 mice) were cut on a Cryostat NX50 (Leica, Milton Keynes, UK). Sections were attached to RNase free PEN-membrane slides (Leica, Milton Keynes, UK) and stained with fast HE staining (ScyTek Labs, UT, USA). A total of 30 Purkinje cells (somatic and proximal dendritic tissue) were isolated from each side of the cerebellum in three slices per animal, for each group (360 Purkinje cells and six RNA samples per genotype; two mice per group) and collected in the tube using Leica LMD 6500 for subsequent RNA isolation. Total RNAs were extracted using Arcturus Picopure RNA isolation Kit (Thermo Fisher, VA, USA). All samples passed Bioanalyzer RNA QC analysis (Agilent, CA, USA), with a RNA integrity Number (RIN) of at least 7. Each 25 μL reaction solution contained 2 μL primers, 12.5 μL SYBR green Supermix (BioRad, CA, USA) and was run under the following conditions: 50°C 2 min; 95°C 10 min; 95°C 15 s, 60°C 1 min for 40 cycles, followed by a melting curve. As a relative quantification, fold changes were measured using the ΔΔCt method, with Gapdh as an internal control.
+ Open protocol
+ Expand
2

Laser Microdissection of Purkinje Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen cerebellar sections (10 um thick) were cut on a Cryostat NX50 (Leica, Milton Keynes, UK). Sections were attached to RNase free PEN-membrane slides (Leica, Milton Keynes, UK) and were stained with fast HE staining. 10 PCs were isolated from each slice, from 3 animals, for each group, and collected in the tube using Leica LMD 6500 for subsequent RNA isolation. Total RNAs were extracted using Arcturus Picopure RNA isolation Kit (Thermo fisher, VA, USA). All samples passed Bioanalyzer RNA QC analysis (Agilent, CA, USA) with a RNA integrity Number (RIN) of at least 7.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!