U87MG cells were seeded at 100000 cells per well into six-well plates and grown for 24 h. Transfection of siRNA (50 nM) was performed using Lipofectamine 2000 (Thermo Fisher Scientific). SignalSilence p38 MAPK siRNA (#6564) and SignalSilence control siRNA (#6568) from “Cell Signaling Technology” were used. Control siRNA (Fluorescein conjugate) (#6201) was added to both preparations (at ratio 1:5). 48 h after transfection, cells were treated by CBD for 6 h (for Western blot analysis) or 24 h for cell-cycle apoptosis analysis using PI staining of fixed cells and the subsequent FACS analysis of green transfected cells.
Signalsilence p38 mapk sirna
Manufactured by Cell Signaling Technology
SignalSilence p38 MAPK siRNA is a small interfering RNA (siRNA) product designed to specifically target and silence the expression of the p38 MAPK gene. It is intended for use in cell-based experiments to study the role of p38 MAPK in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Signalsilence control sirna, by Cell Signaling Technology (1 mentions)
Signalsilence jnk sirna, by Cell Signaling Technology (1 mentions)
Scrambled duplex control sirna, by Cell Signaling Technology (1 mentions)
Mirus transit tko reagent, by Mirus Bio (1 mentions)
2 protocols using signalsilence p38 mapk sirna
1
Investigating p38 MAPK Signaling in U87MG Cells
2
Silencing MAPK signaling in myocytes
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The expressions of MAPKs were silenced by small RNAs interference technique in this study. SignalSilence p38 MAPK siRNA (#6564, Cell Signaling Tech), SignalSilence p44/42 (Erk1/2) siRNA (#6560, Cell Signaling Tech) and SignalSilence JNK siRNA (#6232, Cell Signaling Tech) were used to transfect the cells. The scrambled duplex control siRNA (#6568, Cell Signaling Tech) was used as a negative control. The siRNAs were introduced to cultured myocytes by transfection with Mirus TransIT-TKO reagent (Mirus) in six-well culturing dishes according to the instructions provided by the manufacturer. The final concentrations of siRNAs were 100 nmol/L.
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