Roscovitine

Manufactured by MedChemExpress

Roscovitine is a small molecule that functions as a cyclin-dependent kinase (CDK) inhibitor. It acts by binding to and inhibiting the activity of several CDK enzymes, including CDK1, CDK2, CDK5, CDK7, and CDK9. Roscovitine has been widely used as a research tool to study the role of CDKs in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using roscovitine

Small Molecule Compound Purchases

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemical compounds were purchased from Selleckchem (LY2603618), ThermoFisher Scientific (puromycin, doxycycline, and geneticin), and MedChemExpress (AZD1775, AZD6738, RO-3306, roscovitine, VX-970, NSC663284, and GDC-0575).

Koppenhafer S.L., Goss K.L., Terry W.W, & Gordon D.J. (2019). Inhibition of the ATR-CHK1 pathway in Ewing sarcoma cells causes DNA damage and apoptosis via the CDK2-mediated degradation of RRM2. Molecular cancer research : MCR, 18(1), 91-104.

+ Open protocol

+ Expand

Evaluating BPTF Inhibitors in 5-FU and Oxa Cancer Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

5-Fluorouracil (5-FU, #51-21-8) was purchased from Sigma-Aldrich. 10,058-F4 (#S715301) and Oxaliplatin (Oxa, #S122412) were purchased from Selleck Chemicals. roscovitine (#17817) was obtained from MedChem Express. BPTF inhibitors [55 (link)] DC-BPi-07 and DC-BPi-11 were kindly provided by Dr. Cheng Luo (Shanghai Institute of Materia Medica, Shanghai, China).

Guo P., Zu S., Han S., Yu W., Xue G., Lu X., Lin H., Zhao X., Lu H., Hua C., Wan X., Ru L., Guo Z., Ge H., Lv K., Zhang G., Deng W., Luo C, & Guo W. (2022). BPTF inhibition antagonizes colorectal cancer progression by transcriptionally inactivating Cdc25A. Redox Biology, 55, 102418.

+ Open protocol

+ Expand

Colony Formation Assay Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The procedure for colony formation assay was carried out as previously described (28 (link), 29 (link)). In short, 1×10³ cells were plated into each well in 6-well plates. The next day, the cells were treated with vehicle or Roscovitine (10 μM, Cat# HY-30237, MedChemExpress). After about 16 days of treatment, the cells were fixed with 4% paraformaldehyde for 15 min, stained with (0.5% w/v) crystal violet for 1 h, and then gently rinsed with running water. The cell colonies with more than 50 cells were counted, and the survival curve was generated using linear regression.

Song C., Zhang J., Liu X., Li M., Wang D., Kang Z., Yu J., Chen J., Pan H., Wang H., Li G, & Huang H. (2022). PTEN loss promotes Warburg effect and prostate cancer cell growth by inducing FBP1 degradation. Frontiers in Oncology, 12, 911466.

+ Open protocol

+ Expand

Evaluating DNA Damage Response Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against pATM S1981 (#5883), pATR S428 (#2853), pCHK1 S345 (#2348), pCHK2 T68 (#2197), pH2A.X S139 (γH2A) (#9718), pHH3 S10 (#9286), p CDC25c S216 (#4901), CDC25c (#4688), cleaved-caspase-3 (#9661), BRCA1 (#9010) and GAPDH (#2118), mouse IgG (#7076), rabbit IgG (#7074) were purchased from Cell Signaling Technologies; Anti-RAD51 (ab88572) from Abcam; Anti-pRPA32 (S4/S8) (A300-245A-T) from Bethyl Laboratories and PE-anti-pHH3 S10 (650807) and AlexaFluor488-anti-pH2A.X S139 was purchased by Biolegend. Antibodies were used at the manufacturer's recommended dilutions. Anti-rabbit IgG conjugated with FITC and Anti-rabbit IgG conjugated with FITC were purchased from Proteintech.
AZD1775 and AZD6738 were kindly provided by AstraZeneca Inc.; NU7441, KU-60019, Palbociclib, Roscovitine and Veliparib were obtained from MedChem Express; the above compounds were all diluted in DMSO. Cisplatin and Paclitaxel were purchased from Hansoh Pharmaceutical Co. (Jiangsu, China) and Zhejiang Haizheng Pharmaceuticals (Zhejiang, China), respectively.

Jin J., Fang H., Yang F., Ji W., Guan N., Sun Z., Shi Y., Zhou G, & Guan X. (2018). Combined Inhibition of ATR and WEE1 as a Novel Therapeutic Strategy in Triple-Negative Breast Cancer. Neoplasia (New York, N.Y.), 20(5), 478-488.

+ Open protocol

+ Expand

FBP1 Regulation by Ubiquitin-Proteasome

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression plasmids for Flag-FBP1, HA-FBP1, HA-Ub and HA-SKP2 had been generated in our lab. The Flag-FBP1 mutant S271A was generated by using the KOD-Plus Mutagenesis Kit (Toyobo). The antibodies used in this study include: FBP1 (Abcam); PTEN, AKT and pS473 AKT (Cell Signaling Technology); SKP2, ERK2 and p27Kip1 (Santa Cruz Biotechnology); Ser/Thr-p (BD Scientific); HA tag (Covance); β-Tubulin and Flag tag (Sigma). The chemicals used include: LY294002 (Invitrogen), MG132 (Millipore), CHX (Sigma) and Roscovitine (MedChemExpress).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!