Renal arteries were homogenized in RIPA buffer (Beyotime Biotechnology, China) to obtain whole cell lysates and centrifuged to isolate protein. Ten micrograms of protein were run in an SDS‐polyacrylamide gel electrophoresis and blotted onto a PVDF membrane. After blocking with 5% non‐fat milk, membranes were incubated with the following primary antibodies: CGRP polyclonal antibody (1:8000; Sigma‐Aldrich, USA; Catalog C8198), β‐NGF (1:1000; R&D Systems Inc., USA; Catalog AF‐556‐NA), GAP‐43 (1:500; BIOSS, China; Catalog BS‐0154R), or β‐actin (1:1000; Boster, China; Catalog BM0627; Clone AC‐15). After incubating with anti‐rabbit HRP‐conjugated IgG (1:2500, Boster, China) for 2 h at room temperature, immunoreactive bands were then visualized by chemiluminescence reagents (ECL; 3‐Biologic, Shanghai, China). The band intensity was quantified using Image J analysis software in a blinded manner, and all bands were normalized to corresponding β‐actin bands.
Anti rabbit hrp conjugated igg
Manufactured by Boster Bio
Sourced in China
Anti‐rabbit HRP‐conjugated IgG is a secondary antibody that is conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to primary rabbit antibodies in immunoassays, such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
2 protocols using anti rabbit hrp conjugated igg
1
Renal Protein Expression Analysis
2
Western Blot Analysis of Cardiac Proteins
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Left ventricular tissues were homogenized in RIPA buffer (Beyotime Biotechnology, China) to obtain whole cell lysates and centrifuged to isolate the protein. Fifty micrograms of protein was subjected to SDS-polyacrylamide gel electrophoresis and blotted onto a PVDF membrane. After blocking with 5% non-fat milk, the membranes were incubated with the following primary antibodies: galectin-3 (Abcam, Shanghai, China), spermidine (Abcam, Shanghai, China) or β-actin (Proteintech, Wuhan, China). Then, after incubation with anti-rabbit HRP-conjugated IgG (1:2,500, Boster, China) or anti-mouse HRP-conjugated IgG (1:2,500, Boster, China) for 1 h at room temperature, the immunoreactive bands were visualized by chemiluminescence reagents (ECL; KeyGEN BioTECH, Nanjing, China). The band intensity was quantified using ImageJ analysis software in a blinded manner, and all bands were normalized to the corresponding β-actin bands.
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