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4 protocols using graphite powder 325 mesh

1

Antimicrobial Activity Evaluation

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Silver nitrate (>99.8%), dopamine hydrochloride (>99.9%), graphite powder (325 mesh, 99.9995%), sodium nitrate (>99.0%), and tris (hydroxymethyl) aminomethane were purchased from Sigma-Aldrich (Sigma-Aldrich, Shanghai, China). E. coli (ATCC 11229) and S. aureus (ATCC 6538) were obtained from Guangdong Microbiology Culture Center (Guangzhou, China). Plate agar (PAC), beef extract, and bacterial peptone were purchased from Guangdong Huankai Microbial Sci. and Tech. Co., Ltd. (Guangzhou, China).
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2

Synthesis of Graphite-based Nanomaterials

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Graphite powder (325 mesh) and manganese chloride (MnCl2·4H2O) were purchased from Sigma. Ferric chloride (FeCl3·6H2O), sodium hydroxide (NaOH), potassium permanganate (KMnO4), ammonia solution, sodium nitrate (NaNO3), sulfuric acid (H2SO4), hydrogen peroxide (H2O2) and ethanol were obtained from HiMedia. All the chemicals purchased were of scientific grade and used directly without any purification.
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3

Synthesis of Graphene Oxide

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Pyrrole monomer and adipic acid were purchased from Aladdin Reagent Company Limited. Graphite powder (325 mesh) was supplied by Sigma-Aldrich Chemicals. The concentrated sulfuric acid (H2SO4), potassium permanganate (KMnO4), sodium nitrate (NaNO3), hydrogen peroxide (H2O2), ethanol, hydrochloric acid (HCl), and ammonium persulfate (APS) were obtained from Sinopharm Chemical Reagent Company Limited. All the chemicals were of analytical reagent grade and used without further purification.
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4

Synthesis and Characterization of Mo-LDH

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The chemicals necessary for the synthesis of the Mo-LDH phase, e.g., magnesium nitrate hexahydrate Mg(NO3)2∙6H2O, aluminum nitrate nonahydrate Al(NO3)3∙9H2O, sodium molybdate dihydrate Na2MoO4∙2H2O, anhydrous sodium carbonate Na2CO3, and sodium hydroxide NaOH (pearls), were all of chemical purity grade and were purchased from Merck (Darmstadt, Germany). For the preparation of the GO phase, graphite powder 325 mesh from Sigma–Aldrich (Saint Louis, MO, USA), sodium nitrate NaNO3, potassium permanganate KMnO4 (chemical purity from Merck), H2SO4 (98%), hydrochloric acid HCl 37% (from Merck), and hydrogen peroxide H2O2 30% (from ChimReactiv, Bucharest, Romania) were utilized.
Indigo carmine (IC) from Sigma–Aldrich (Saint Louis, MO, USA) was used to prepare the simulated dye-contaminated water in the laboratory.
All the aqueous solutions were obtained using distilled water with a conductivity of 2.5–5 μS/cm.
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