Monoclonal anti flag antibody f3165

Anti-RUNX2 antibody (SC-390351), anti-SAMD4 antibody (SC-163329) and anti-TUBULIN antibody (SC-23948) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); monoclonal anti-Flag antibody (F3165) was from Sigma-Aldrich; anti-MIG6 antibody (11630) was from ProteinTech, Rosemont, IL, USA; and anti-Tnfrsf12a (ab109365) was from Abcam (San Francisco, CA, USA).

Anti-Set8 (C18B7; 1:1,000 immunoblot (IB)), anti-β-catenin (D10A8; 1:1,000 IB), anti-Skp2 (D3G5; 1:1,000 IB), anti-Mcl-1 (D35A5; 1:1,000 IB), anti-G9A (C6H3; 1:1,000 IB), anti-Cullin 1 (4995; 1:1,000 IB) and anti-β-TRCP1 (4394; 1:1,000 IB) antibodies were purchased from Cell Signaling Technology. Anti-p27 (SC-528; 1:500 IB), anti-Cullin 1 (SC-17775; 1:1,000 IB), anti-casein kinase 1δ (H-60; 1:1,000 IB), anti-casein kinase 1α (SC-6474; 1:1,000 IB), anti-c-Myc (9E10; 1:1,000 IB, 2 μg immunoprecipitates (IP)) and polyclonal anti-HA antibodies (SC-805; 1:1,000 IB, 2 μg IP) were purchased from Santa Cruz Biotechnology. Anti-Vinculin antibody (V-4505; 1:2,000 IB), monoclonal anti-Flag antibody (F-3165), polyclonal anti-Flag antibody (F-2425; 1:1,000 IB, 2 μg IP), anti-HA agarose beads (A-2095; 1:1,000 IB, 2 μg IP), peroxidase-conjugated anti-mouse secondary antibody (A-4416; 1:3,000 IB) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914; 1:3,000 IB) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Anti-GFP antibody (632380; 1:1,000 IB), anti-Cdh1 (34-2000; 1:1,000 IB) antibody, Oligofectamine, Lipofectamine and Plus reagents were purchased from Invitrogen. Anti-CUL 4B (16184-1-AP; 1:1,000 IB) antibody was purchased from Proteintech Group.

Anti-RANKL antibody (ab45039; 1:1,000) was obtained from Abcam. Anti-SMAD3 (C67H9) antibody (9523, s; 1:1,000) and anti P-SMAD3 (S423/425) antibody (9520, s; 1:1,000) were purchased from Cell Signaling Technology. Monoclonal anti-HA antibody (SC-7392; 1:2,000), anti-VDR rabbit polyclonal antibody (SC-1008; 1:1,000), anti-VDR mouse monoclonal antibody (SC-13133; 1:1,000) and anti-TUBULIN antibody (SC-23948; 1:10,000) was from Santa Cruz Biotechnology. Anti-SMURF2 antibody (ABS59; 1:500) was obtained from Millipore. Monoclonal anti-FLAG antibody (F3165; 1:5,000) was from Sigma-Aldrich. Monoclonal anti-MYC antibody (AE010; 1:2,000) was from Abclonal Technology. Monoclonal anti-GFP antibody (66002-1; 1:10,000) was purchased from Proteintech. Rabbit IgG (SC-2027) was purchased from Santa Cruz Biotechnology and Mouse IgG (I5381) was from Sigma-Aldrich. Recombinant mouse TGF-β1 (catalogue no.5231) was purchased from Cell Signaling Technology; recombinant human BMP-2(120-02) was from Proteintech; β-oestradiol (oestrogen; 02194565.2) was from MP. SB525334 was purchased from Selleckchem.