Charcoal dextran stripped fetal bovine serum cd fbs

Reagents were of ACS grade or higher (e.g. HPLC and Optima LC/MS) and were purchased from Thermo Fisher Scientific and used without further purification. Testosterone, epiTestosterone (epi-T), DHEA, DHEA-S sodium salt, DHEA glucuronide (DHEA-G), AST, epi-AST, DHT, 5-adiol, 3α-adiol and 3β-adiol were purchased from Steraloids (Wilton, NH, USA). [2,3,4-¹³C₃]-T ([¹³C₃]-T) and [2,3,4-¹³C₃]-DHT ([¹³C₃]-DHT) were from C/D/N Isotopes (Point-Claire, Quebec, Canada) and Cambridge Isotopes (Andover, MA, USA), respectively. [2,3,4-¹³C₃]-3α-adiol ([¹³C₃]-3α-adiol) and [2,3,4-¹³C₃]-3β-adiol ([¹³C₃]-3β-adiol) were synthesized by an enzymatic method according to our published procedure (Zang et al. 2017 (link)). 4-Dimethylaminopyridine (DAP) 2-methyl-6-nitrobenzoic anhydride (MNBAn), picolinic acid (PA), triethylamine (TEA), anhydrous tetrahydrofuran (THF), β-glucuronidase from E. coli and sulfatase from Abalone entrails were from Sigma-Aldrich. Charcoal dextran stripped fetal bovine serum (CD-FBS) was from Atlanta Biologicals (Lawrenceville, GA, USA).

Reagents were of ACS grade or higher and were purchased from Fisher Scientific (Pittsburgh, PA, USA) and used without further purification. [1β-³H(N)]-Δ⁴-androstene-3,17-dione (4-AD) (Specific Radioactivity 96.2 Ci/mole) and [³H-R1881 (85.1 Ci/mmole) were obtained from Perkin-Elmer. 4-Androstene-3,17-dione and dihydrotestosterone were purchased from Steraloids and Sigma, respectively. Unlabeled R1881 was obtained from Perkin-Elmer. Nicotinamide adenine dinucleotide, Grade I reduced form (NADH) and nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) were obtained from Roche Diagnostics (Indianapolis, IN, USA). R-biaclutamide was purchased from Sigma; Enzalutamide was obtained from Selleckchem; and abiraterone was purchased Euroasia Chemicals (Mumbai - 400 013, India ). Charcoal dextran stripped fetal bovine serum (CD-FBS) was from Atlanta Biologicals (Lawrenceville, GA, USA).