Primary human umbilical vein endothelial cells (HUVECs; Thermo Fisher Scientific, Rockford, IL, USA), from pooled donors were cultured in VascuLife basal medium supplemented with VascuLife VEGF Life factors kit (Lifeline Cell Technology, GmbH, Troisdorf, Germany) in a humidified incubator with 5% CO2 at 37 °C. The cells between passages 4 and 8 were used in the experiment.
Vasculife vegf lifefactors kit
Manufactured by Lifeline Cell Technology
Sourced in Germany, United States
The VascuLife VEGF LifeFactors Kit is a laboratory product that provides a defined formulation of growth factors and supplements for the culture of vascular endothelial cells. The kit contains a combination of essential components required for the optimal growth and maintenance of these cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Male c57b 6j mice, by Jackson ImmunoResearch (2 mentions)
Huvecs, by Thermo Fisher Scientific (1 mentions)
Ebm 2 basal medium, by Lonza (1 mentions)
Egm 2 singlequot, by Lonza (1 mentions)
Nitrate nitrite colorimetric assay kit, by Cayman Chemical (1 mentions)
Glutaraldehyde, by Electron Microscopy Sciences (1 mentions)
Cyquant ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions)
Vasculife basal medium, by Lifeline Cell Technology (1 mentions)
Hexamethyldisilazane hmds, by Electron Microscopy Sciences (1 mentions)
Huvecs, by American Type Culture Collection (1 mentions)
5 protocols using vasculife vegf lifefactors kit
1
Culturing Primary Human Umbilical Vein Endothelial Cells
2
Isolation and Culture of Human Neutrophils and Endothelial Cells
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Blood neutrophils from healthy human donors and human umbilical vein endothelial cells (HUVECs) were obtained according to the operational guidelines of, and after due approval by the Charité (Ethics Votum EA1/277/11). Prior written, informed consent from all subjects was obtained as described previously5 (link). Neutrophils from the oral cavity were harvested as described by Ashkenazi39 (link). HUVECs were cultured in EBM-2 basal medium, supplemented with EGM-2 SingleQuots (Lonza) and used after two to four passages. Primary Human glomerular Microvascular Endothelial Cells (gMVEC) were obtained from Cell Systems (Kirkland, WA) and cultured in VascuLife basal medium, supplemented with VascuLife VEGF LifeFactors Kit (Lifeline Cell Technology, Frederick, MD).
3
Murine HUVEC Isolation and Culture
4
Biocompatible PCL Scaffold Characterization
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All chemicals were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA) if not mentioned otherwise. Medical grade PCL filament was purchased from Advanced Biomedical Technology Inc. (Hsinchu City, Taiwan). The Nitrate/Nitrite Colorimetric Assay Kit was purchased from Cayman Chemical (Ann Arbor, MI, USA). The CyQUANT™ LDH Cytotoxicity Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The VascuLife® Basal Medium and the VascuLife® VEGF LifeFactors Kit were purchased from Lifeline Cell Technology (Frederick, MD, USA). The NucSpot® 470 Nuclear Stain, 1000x in DMSO, was purchased from Biotium, Inc. (Fremont, CA, USA). The EdU-Click 594 Cell Proliferation Kit was purchased from baseclick GmbH (Munich, Germany). The lab-grade BaSO4 was purchased from Lab Alley (Spicewood, TX, USA). The acetone was purchased from Lab Chem Inc. (Zelienople, PA, USA). The hexamethyldisilazane (HMDS), as well as the glutaraldehyde, were purchased from Electron Microscopy Sciences (Hatfield, PA, USA). The phosphate buffered saline (PBS) was purchased from Life Technologies Limited (Paisley, PA, USA). The formaldehyde was purchased from Alfa Aesar Thermo Fisher Scientific (Ward Hill, MA, USA).
5
Mouse Endothelial Cell Culture
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Male C57B/6J mice (8‐12 weeks old) were purchased from Jackson Laboratory. All animal experiments were performed according to Wayne State University Institutional Animal Care and Use Committee guidelines. The primary human umbilical vein endothelial cells (HUVECs) from four different donors were obtained from the American Type Culture Collection. Cells were grown in vascular cell basal media (VCBM) supplemented with VascuLife VEGF LifeFactors Kit (Lifeline Cell Technology). Only cells between passages 2 to 5 were used for experiments.
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