Pde4b

Manufactured by BPS Biosciences

Sourced in United States

PDE4B is a recombinant human phosphodiesterase 4B (PDE4B) enzyme. PDE4B hydrolyzes the second messenger cyclic adenosine monophosphate (cAMP) to 5'-AMP, thereby regulating cellular cAMP levels.

Automatically generated - may contain errors

Lab products found in correlation

Pde glo phosphodiesterase assay, by Promega (1 mentions) Pde4d, by BPS Biosciences (1 mentions) Pde7a, by BPS Biosciences (1 mentions) Pde1a, by BPS Biosciences (1 mentions) Pde3a, by BPS Biosciences (1 mentions) Lance ultra camp assay kit, by PerkinElmer (1 mentions) Envision multilable reader, by PerkinElmer (1 mentions)

2 protocols using pde4b

Inhibitory Activity of Brilacidin on PDE4

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Example 11

To examine the inhibitory activity of brilacidin on PDE4 phosphodiesterase, inhibition assays of PDE4 were performed using brilacidin. The PDE-Glo phosphodiesterase assay (Promega, Madison, Wis.) was performed using 8 ng of PDE4B3, 1 μM cAMP substrate and brilacidin. The compounds and PDE4B (BPS Biosciences, San Diego, Calif.) were mixed and pre-incubated at room temperature for 15 minutes. Substrate was added and the reaction was incubated for 10 minutes at room temperature. Data are presented as luminescence units (RLU). Brilacidin inhibited PDE4 in a dose dependent manner with an IC50 in the 3 μM range (FIG. 10, Panels A and B, semi-logarithmic and linear axes respectively). This is the first demonstration of a HDP mimetic inhibiting a PDE.

US11771694B2. Arylamide compounds for treatment and prevention of viral infections (2023-10-03). Innovation Pharmaceuticals Inc. [US]. Inventors: Jane A. Harness [US], Leo Ehrlich [US], Warren Kyle Weston [US].

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Enzyme Inhibition Assay for PDE Enzymes

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The enzyme inhibition assay was performed against human PDE enzymes (PDE1A, PDE3A, PDE4A, PDE4B, PDE4C, PDE4D, and PDE7A; BPS Biosciences, San Diego, CA, United States) according to the manufacturer’s instructions (LANCE Ultra cAMP assay kit; Perkin Elmer, United States). In each well, 5 μl of 3 nM cAMP, 2.5 μl of PDE enzyme (0.1 ng/well), and 2.5 μl of inhibitor solution were added, and incubated at 37°C for 1 h. After incubation, 5 μL each of Eu-cAMP and ULight-anti-cAMP detection reagent supplemented with 1 mM of IBMX were added. The reaction mixture was incubated at 37°C for 1 h. After incubation, emission signals were collected at 665 nm using EnVision Multilable Reader (Perkin Elmer, United States).

Purushothaman B., Arumugam P, & Song J.M. (2018). A Novel Catecholopyrimidine Based Small Molecule PDE4B Inhibitor Suppresses Inflammatory Cytokines in Atopic Mice. Frontiers in Pharmacology, 9, 485.

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