At 82 weeks of age, 28 blood samples were collected from the brachial vein into heparinized tubes at 12 PM from 7 fasted birds per each group. All birds were fasted to 6 h before blood collection. Blood plasma was obtained by centrifugation for 20 min at 3000 rpm and stored at −20 °C until analysis and then allowed to thaw at room temperature (20 °C) before analysis. Total protein and albumin levels were measured by using commercial kits as per the manufacturer’s recommendations using a Biochemistry Auto Analyzer (Sinnowa D280, Nanjing, China).
The concentration of plasma MDA was determined by a commercial colorimetric kit (malondialdehyde; Biodiagnostic Co., Cairo, Egypt) according to Satoh [24 (link)], while total antioxidant capacity was measured using a colorimetric kit (Biodiagnostic Co., Cairo, Egypt). The total lipids contents were estimated as described by Bligh and Dyer [25 (link)].
The concentration of plasma MDA was determined by a commercial colorimetric kit (malondialdehyde; Biodiagnostic Co., Cairo, Egypt) according to Satoh [24 (link)], while total antioxidant capacity was measured using a colorimetric kit (Biodiagnostic Co., Cairo, Egypt). The total lipids contents were estimated as described by Bligh and Dyer [25 (link)].