Log In Sign up
The largest database of trusted experimental protocols

Proxeon easy nlc 1200 uhplc system

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Proxeon EASY-nLC 1200 UHPLC system is a high-performance liquid chromatography instrument designed for the separation and analysis of complex samples. It features a flow rate range of 50 nL/min to 50 μL/min and a maximum pressure of 1200 bar, enabling the use of narrow-bore columns for efficient separation and high-resolution analysis.

Automatically generated - may contain errors

Lab products found in correlation

Picofrit 10um tip opening, by New Objective (2 mentions) Reprosil pur c18 aq beads, by Dr. Maisch (2 mentions)

Close spelling variants of this product

EASY-nLC 1200 (450 citations) Easy nLC (237 citations) EASY-nLC system (111 citations) UHPLC system (58 citations) Proxeon nLC-1200 (3 citations)

2 protocols using proxeon easy nlc 1200 uhplc system

1

Nanoscale Proteome Separation and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Online separation was done with a nanoflow Proxeon EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific). In this set up, the LC system, column, and platinum wire used to deliver electrospray source voltage were connected via a stainless-steel cross (360 μm, IDEX Health & Science, UH-906x). The column was heated to 50°C using a column heater sleeve (Phoenix-ST) to prevent over-pressuring of columns during UHPLC separation. Each peptide fraction containing ~1ug (based on protein-level BCA prior to digestion, with uniform distribution of fraction content presumed), the equivalent of 12% of each global proteome sample in a 2 ul injection volume or 50% of each phosphoproteome sample in a 4 ul injection volume, was injected onto an in-house packed 20cm x 75um diameter C18 silica picofrit capillary column (1.9 μm ReproSil-Pur C18-AQ beads, Dr. Maisch GmbH, r119.aq; Picofrit 10um tip opening, New Objective, PF360–75-10-N-5). Mobile phase flow rate was 200 nL/min, comprising 3% acetonitrile/0.1% formic acid (Solvent A) and 90% acetonitrile/0.1% formic acid (Solvent B). The 110-minute LC-MS/MS method consisted of a 10-min column-equilibration procedure, a 20-min sample-loading procedure, and the following gradient profile: (min:%B) 0:2; 1:6; 85:30; 94:60; 95;90; 100:90; 101:50; 110:50 (the last two steps at 500 nL/min flow rate).
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
+ Open protocol
+ Expand
2

Nanoscale Proteome Separation and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Online separation was done with a nanoflow Proxeon EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific). In this set up, the LC system, column, and platinum wire used to deliver electrospray source voltage were connected via a stainless-steel cross (360 μm, IDEX Health & Science, UH-906x). The column was heated to 50°C using a column heater sleeve (Phoenix-ST) to prevent over-pressuring of columns during UHPLC separation. Each peptide fraction containing ~1ug (based on protein-level BCA prior to digestion, with uniform distribution of fraction content presumed), the equivalent of 12% of each global proteome sample in a 2 ul injection volume or 50% of each phosphoproteome sample in a 4 ul injection volume, was injected onto an in-house packed 20cm x 75um diameter C18 silica picofrit capillary column (1.9 μm ReproSil-Pur C18-AQ beads, Dr. Maisch GmbH, r119.aq; Picofrit 10um tip opening, New Objective, PF360–75-10-N-5). Mobile phase flow rate was 200 nL/min, comprising 3% acetonitrile/0.1% formic acid (Solvent A) and 90% acetonitrile/0.1% formic acid (Solvent B). The 110-minute LC-MS/MS method consisted of a 10-min column-equilibration procedure, a 20-min sample-loading procedure, and the following gradient profile: (min:%B) 0:2; 1:6; 85:30; 94:60; 95;90; 100:90; 101:50; 110:50 (the last two steps at 500 nL/min flow rate).
Krug K., Jaehnig E.J., Satpathy S., Blumenberg L., Karpova A., Anurag M., Miles G., Mertins P., Geffen Y., Tang L.C., Heiman D.I., Cao S., Maruvka Y.E., Lei J.T., Huang C., Kothadia R.B., Colaprico A., Birger C., Wang J., Dou Y., Wen B., Shi Z., Liao Y., Wiznerowicz M., Wyczalkowski M.A., Chen X.S., Kennedy J.J., Paulovich A.G., Thiagarajan M., Kinsinger C.R., Hiltke T., Boja E.S., Mesri M., Robles A.I., Rodriguez H., Westbrook T.F., Ding L., Getz G., Clauser K.R., Fenyö D., Ruggles K.V., Zhang B., Mani D.R., Carr S.A., Ellis M.J, & Gillette M.A. (2020). Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy. Cell, 183(5), 1436-1456.e31.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!