Human NB cell lines (SH-SY5Y, and SK-N-BE(2)) were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China), and verified by short tandem repeat analysis. Cells were cultured in DMEM/F12 (Thermo Fisher Scientific) supplemented with 10% FBS (Biological Industries, USA) and 1% penicillin-streptomycin (Beyotime, China) at 37 °C with a humidified atmosphere of 5% CO2 and examined free of mycoplasma regularly.
Sh sy5y
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The SH-SY5Y is a human-derived neuroblastoma cell line. It is a subclone of the parental line SK-N-SH, which was originally isolated from a bone marrow biopsy of a 4-year-old female with neuroblastoma. The SH-SY5Y cell line is commonly used in neuroscience research as a model for studying neuronal function and disease.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Sh sy5y, by Thermo Fisher Scientific (4 mentions)
Sh sy5y, by Merck Group (3 mentions)
Imr 32, by National Collection of Authenticated Cell Cultures (3 mentions)
Sk n be 2, by National Collection of Authenticated Cell Cultures (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Beyotime (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Aβ25 35, by Merck Group (2 mentions)
Sk n sh, by National Collection of Authenticated Cell Cultures (2 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Mtesr1 medium, by STEMCELL (1 mentions)
Lipo3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Caco 2, by National Collection of Authenticated Cell Cultures (1 mentions)
Dmem basal medium, by Thermo Fisher Scientific (1 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)
Hepg2, by Thermo Fisher Scientific (1 mentions)
22 protocols using sh sy5y
1
Culturing Human NB Cell Lines
2
Cell Culture Protocols for Research
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HeLa, SH-SY5Y, 293T, and U2OS cells were all purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). They were cultured in DMEM-high glucose (Gibco, Carlsbad, CA, USA), which was supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin at 37 °C in 5% CO2. In addition, human embryonic stem cells (ESCs) H9 were obtained from Dr. Hongkui Deng, Peking University Stem Cell Research Center (Beijing, China) [17 (link)]. Coat plates with 1% matrigel (354277, Corning, New York, NY, USA) were heated at 37 °C for 1 h, cultured in mTeSR™1 medium (85850, STEMCELL, Vancouver, Canada), and supplemented with 1% penicillin/streptomycin and Y27632 (10 μM).
3
Cell Culture of Human Cell Lines
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The human NB cell lines (SK-N-BE(2) and SH-SY5Y) and human 293FT cells were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China). Cells were cultured in DMEM/F12 supplemented with 10% FBS (all from Gibco-BRL, Grand Island, NY, United States) and 1% penicillin‒streptomycin (Beyotime Biotechnology, Shanghai, China) at 37°C in a humidified atmosphere containing 5% CO2.
4
Cultivation and Transfection of Diverse Cell Lines
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Vero E6, A549, SH-SY5Y, HepG2, Caco2, L929, and SCC7 cells were purchased from National Collection of Authenticated Cell Cultures, China. H1299 and H1299 AXL–/– cells were obtained as a gift from Prof. Xu Li (Westlake University, Hangzhou, China). Vero E6, SH-SY5Y, HepG2, Caco2, and L929 cells were grown in 90% DMEM basal medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and 100 units/mL penicillin/streptomycin (Gibco, USA). A549, SCC7, H1299 and H1299 AXL–/– cells were grown in RPMI-1640 basal medium (Hyclone, USA) supplemented as above. All cells were cultured in 5% CO2 incubator at 37 °C. Lipo3000 transfection reagent (Thermo Fisher, USA) was used for transient transfection of DNA constructs according to the manufacturer’s instructions.
5
Murine Lung Endothelial Cell Cultivation
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The murine lung endothelial cell (MLEC) lines were as previously reported[15 (link)].
The cell lines U87, SH-SY5Y, A549, MRC-5, WI-38, HuH-7, SK-HEP-1, Hep3B2.1–7, HT1080, EA.hy926, and HEK293 are ordered from National Collection of Authenticated Cell Cultures (Shanghai, China). All cells were cultured in medium supplemented with 10% fetal bovine serum (Gibco, 10099141C), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen, USA).
The cell lines U87, SH-SY5Y, A549, MRC-5, WI-38, HuH-7, SK-HEP-1, Hep3B2.1–7, HT1080, EA.hy926, and HEK293 are ordered from National Collection of Authenticated Cell Cultures (Shanghai, China). All cells were cultured in medium supplemented with 10% fetal bovine serum (Gibco, 10099141C), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen, USA).
6
SH-SY5Y Neuroblastoma Cell Transfection
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The neuroblastoma cell line SH-SY5Y was obtained from National Collection of Authenticated Cell Cultures and grown in DMEM (#L110KJ, Basalmedia, Shanghai, China) with 10% FBS (#CCS30009.02 500 mL, EN MOASAI BIOLOGICAL TECHNOLOGY CO., LTD, Changzhou, China ) at cell incubator. Plasmid DNA transfection was performed according to the manufacturer’s guidelines of Lipofectamine 2000 (#11668019, Invitrogen, Carlsbad, CA, USA).
7
Neuroblastoma Cell Line for Neurotoxicity
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Because primary neurons cannot be trypsinized and reseeded, the human neuroblastoma cell line SH-SY5Y may be adopted as a substitution in the study of neurotoxicity. The SH-SY5Y cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum and maintained in a humidified incubator at 37 °C in a 5% CO2 atmosphere. For flow cytometry analysis and microscopic observation, the cells were trypsinized and plated at a density of 1×106/well in 6-well plates or 5×104/well in 24-well plates.
8
Neuroblastoma Cell Differentiation Assay
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Human neuroblastoma cell line SH-SY5Y was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were maintained at 37 C in Dulbecco's modified Eagle's Medium (DMEM) supplemented with 10% newborn calf serum under a humidified atmosphere of 5% CO 2 . The SH-SY5Y cells were treated with 5 mM all-trans-retinoic acid (ATRA) (Sigma, St. Louis, MO) in DMEM in the dark for 6 d to induce neuronal differentiation. The cells with axon lengths that were twice those of their neuronal cell bodies were regarded as positive cells. Six visual fields were randomly selected from each slide by two blinded observers. At least 200 cells were counted. The differentiated cells were divided into four groups: control group, model group, SH-SY5Y group, and SH-SY5Y + LY294002 (10 mM) group (n ¼ 6 per group).
9
Regulating miR-410 in Neuronal Cells
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A rat pheochromocytoma cell line PC12 and a human neuroblastoma cell line SH‐SY5Y were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen, Thermo Fisher Scientific, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Thermo Fisher Scientific, CA, USA) at 37°C in a humidified incubator with 5% CO2. To regulate the expression of miR‐410 in neuronal cells, PC12 and SH‐SY5Y cells were transfected with miR‐410 mimic or mimic negative control (NC) by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) following the manufacturers’ protocols. The miR‐410 mimic and mimic NC sequences were synthesized by GenePharma (Shanghai, China).
10
Alzheimer's Disease Cell Model
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A human neuroblastoma cell line SH-SY5Y and a human renal epithelial cell line
HEK293 were purchased from the Cell Bank of Type Culture Collection of Chinese
Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified
Eagle’s medium (DMEM; Invitrogen, Thermo Fisher Scientific, CA, USA) in a
humidified incubator with 5% CO2 at 37 ℃. For the construction of AD
cell model, SH-SY5Y cells were harvested and treated with 1 µM of Aβ25-35
(Sigma-Aldrich, Saint Louis, MO, USA) for 24 h to simulate AD progression.
HEK293 were purchased from the Cell Bank of Type Culture Collection of Chinese
Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified
Eagle’s medium (DMEM; Invitrogen, Thermo Fisher Scientific, CA, USA) in a
humidified incubator with 5% CO2 at 37 ℃. For the construction of AD
cell model, SH-SY5Y cells were harvested and treated with 1 µM of Aβ25-35
(Sigma-Aldrich, Saint Louis, MO, USA) for 24 h to simulate AD progression.
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