Esi jet stream ion source

The separation samples were analyzed by an HPLC/ESI-QTOF-MS system in positive ion mode using a 6530B Accurate-Mass-QTOF-MS mass spectrometer with an ESI-Jet Stream^® ion source and Atlantis HILIC silica column (150 × 2.1mm, dp = 3μm) (Waters Milford, MA, USA). The liquid chromatography system was equipped with DAD (diode array detector), autosampler, binary gradient pump, and column oven. Gradient of solvents: acetonitrile (95%) with 10 mM ammonium formate (0.2%) (solvent A) and acetonitrile (50%) with 10 mM ammonium formate (0.2%) (solvent B) was used as the mobile phase. The following gradient procedure was adopted: 0–10 min, 100% using solvent A; 10–40 min, 92% A and 8% B; 40–45 min, 64% A and 36% of solvent B. ESI-QTOF-MS analysis was performed according to the following parameters: total time of analysis was 45 min, with a stable flow rate at 0.25 mL/min. Injection volume for analyzed extracts was 10 μL. Dual spray jet stream ESI, (+)-positive ion mode, gas (N₂) flow rate: 12 L/min., nebulizer pressure: 35 psig, vaporizer temp.: 300 °C; m/z range 100–1000 mass units, with acquisition Mode AutoMS², collision induced dissociation (CID): 0, 10, 40 and 60 eV with MS scan rate 1 spectrum per s, 2 spectra per cycle, skimmer: 65 V, fragmentor: 150 V and octopole RF Peak: 750 V.

The PAs–PANOs extract was examined by qualitative LC/MS analysis. The scientific equipment used was an Agilent | 6500 Series Accurate-Mass Quadrupole Time-of-Flight (Agilent Technologies Inc., Santa Clara, CA, USA) device equipped with ESI-Jet Stream ion source and Atlantis HILIC silica column (150 × 2.1 mm, dp = 3 μm) (Waters Milform, MA, USA). The chromatograph used a diode array detector autosampler, dual grading pump, and column heater. An RP-18 stable phase was used in conjunction with a gradient elution mobile phase of 0.1% formic acid in MeOH with a stable flow of 0.25 mL/min. For this particular experiment, a positive charge ESI ion source was used [40 (link)].

The PA/PANO extract was examined by qualitative LC/MS analysis. The scientific equipment used was an Agilent 6500 Series Accurate-Mass Quadrupole Time-of-Flight (Agilent Technologies Inc., Santa Clara, CA, USA) device equipped with ESI-Jet Stream ion source and Atlantis HILIC silica column (150 × 2.1 mm, dp = 3 μm) (Waters, Milford, MS, USA). The chromatograph used a diode array detector autosampler, dual grading pump, and column heater. An RP18 stable phase was used in conjunction with a gradient elution mobile phase of 0.1% formic acid in methanol with a stable flow of 0.25 mL/min. For this particular experiment the MS scans were acquired in positive ionization mode.