Genotyping was performed using the Fluidigm 96.96 Dynamic Array IFCs (Fluidigm, San Francisco, CA, United States) [33 (link)]. Cases and controls were plated out in sets of 96 samples and combined into 384-well arrays for genotyping. Polymerase chain reaction (PCR) was performed in a 5-μl reaction and cycling conditions were set using the standard procedure according to the manufacturer's protocol. To obtain genotype calls, we analyzed the data using EP1 SNP Genotyping Analysis software. The software defined the genotype of each sample based on the relative fluorescence intensities.
Fluidigm 96.96 dynamic array ifcs
Manufactured by Standard BioTools
Sourced in United States
The Fluidigm 96.96 Dynamic Array IFCs is a lab equipment product designed for gene expression analysis. It features a microfluidic chip with 96 sample inlets and 96 assay inlets, enabling simultaneous measurement of up to 9,216 individual gene expression reactions in a single experiment.
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Lab products found in correlation
2 protocols using fluidigm 96.96 dynamic array ifcs
1
Genotyping Using Fluidigm 96.96 Dynamic Array
2
Genotyping of JAG1 SNPs in Chinese
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Tag single nucleotide polymorphisms (SNPs) for JAG1 were selected using the SNPinfo Web Server with minor allele frequency ≥0.01 and r2 ≥ 0.8 as the selection criteria based on the HapMap Chinese Han in Beijing data. A total of 31 tag SNPs were selected (Supplementary Table S1 ) and genotyped in 333 cases and 1,665 controls using Fluidigm 96.96 Dynamic Array IFCs (Fluidigm, San Francisco, CA, United States) (Supplementary Table S2 ). The HaploRegv4.1 database was used to predict the function of the associated SNPs (Supplementary Table S3 ).
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