Male C57BL/6 mice (6-8 weeks, 20-22 g) were bought from Jiangsu GemPharmatech Co., Ltd. (Nanjing, China), and bred in standard laboratory conditions in the Tongji Hospital Laboratory Animal Centre. All animal experiments were permitted by the Animal Ethics Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Sitagliptin was purchased from MedChemExpress (New Jersey, USA).
Male c57bl 6 mice
Manufactured by GemPharmatech
Sourced in China
Male C57BL/6 mice are laboratory animals commonly used in research. They are a widely used inbred strain of house mouse (Mus musculus) that serves as a standardized model organism. These mice are primarily maintained for their genetic stability and predictable physiological responses, which make them useful in a variety of scientific studies.
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12 protocols using male c57bl 6 mice
1
Sitagliptin Treatment in C57BL/6 Mice
2
Acetaminophen-Induced Liver Injury in Mice
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Male C57BL/6 mice (18–22 g, 5–7 weeks old) used in this research were obtained from GemPharmatech Co., Ltd. (Nanjing, China). The animals were housed five per cage under standard laboratory conditions, with free access to a regular commercial diet and water. The experiment was carried out in the institutional animal care and use committee of the First Affiliated Hospital of Zhejiang University. APAP (HY-66005), NAC (HY-B0215) and Anisomycin (HY-18982) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). All animals were fasted overnight before APAP administration and were administered a single dose of 500 mg/kg APAP by intraperitoneal (i.p.) injection to establish a mouse model of APAP-induced liver injury. NAC (300 mg/kg via i.p. injection) and AMSCs (2 × 105 via tail vein) were treated immediately after APAP administration. A JNK activator, Anisomycin (20 mg/kg), was administered via i.p. injection 30 min prior to APAP injection to further investigate the role of the JNK pathway in mediating the protective effect of AMSCs. For AMSCs delayed treatment assay, AMSCs were injected 1 h, 2 h, 4 h and 6 h after APAP overdosing. Mice were killed 6 and 24 h after receiving APAP treatment. Serum samples and liver tissues were harvested. Liver tissues were flash frozen in liquid nitrogen and stored at −80 °C for further use.
3
Murine Model of Myocardial Infarction
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Male C57BL/6 mice (aged 8 weeks old) were purchased from Gempharmatech (Nanjing, China) and maintained in a standard laboratory environment (70% relative humidity, 22 °C, a 12:12‐hour light–dark cycle) at the Cardiovascular Research Institute of Renmin Hospital of Wuhan University. All mice were supplied with sterile water and rodent food and acclimatized to the new environment for 2 weeks. Then, mice were were divided into 5 groups: Sham, MI 1 day, MI 3 days, MI 7 days, and MI 14 days. Animals were euthanized by CO2 inhalation on day 1, 3, 7, and 14 after MI surgery, respectively, and all efforts were made to minimize suffering. Then, brain tissues were immediately isolated for detailed analysis.
4
Microglia Depletion and Myocardial Infarction
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Male C57BL/6 mice (aged 6 weeks old) were purchased from Gempharmatech and maintained at the Cardiovascular Research Institute of Renmin Hospital of Wuhan University, as described above. All mice were acclimatized to the new environment for 2 weeks. First, to specifically induce microglia depletion in the CNS and explore the dose of PLX3397 via intracerebroventricular injection, mice were administered 2 μL PLX3397 (dissolved in DMSO, once per day for 7 days) via intracerebroventricular injection at doses of 5, 10, 25, and 50 μg per mouse, respectively. Then, PLX3397 (intracerebroventricular, 50 μg, once per day for 7 days) was used to deplete microglia in subsequent experiments. Mice in the control group were administered DMSO (MedChemExpress America). After administration of PLX3397 (intracerebroventricular) or DMSO (intracerebroventricular) for 7 days, the mice were divided into 4 groups: Sham+DMSO (intracerebroventricular), Sham+PLX3397 (intracerebroventricular), MI+DMSO (intracerebroventricular), MI+PLX3397 (intracerebroventricular). DMSO or PLX3397 was continued until the end of the experiment. Animals were euthanized by CO2 inhalation on days 3 and 14 after MI surgery, and all efforts were made to minimize suffering. Then, heart, brain, and spleen tissues were immediately isolated for detailed analysis.
5
Murine Tumor Burden Measurement
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Male C57BL/6 mice (18 ± 2 g, 6–8 weeks) were obtained from GemPharmatech in China. Mice were housed in constant environmental conditions (room temperature, 21 ± 1 °C; relative humidity, 40–70%, and a 12 h light–dark cycle). Mice were access to food and water free. All animal experiments were approved by the Institutional Animal Care and Ethics Committee of Sichuan University. The experiment was designed without considering the sex of mice, and male mice were selected to ensure gender uniformity. The maximum tumor burden in mice permitted by the Institutional Animal Care and Ethics Committee of Sichuan University was 2000 mm3. In some cases, this limit has been exceeded by the last day of measurement and the mice were immediately euthanized.
6
Apolipoprotein E Knockout Mouse Model
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Male C57BL/6 mice and male apolipoprotein E knockout (ApoE-/-) mice (six-week-old) were obtained from the GemPharmatech Co., Ltd. Animals were housed in standard mice cages with ad libitum access to water and food. Before experiments, all mice were acclimatized for at least three days. All animal work was performed under the guidelines of the China Council on Animal Care and Sichuan University protocol for animal use. All ethical guidelines for experimental animals were followed.
7
Mouse Model of Vitamin D-Induced Vascular Calcification
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Male C57BL/6 mice (8 weeks old) were purchased from GemPharmatech (Nanjing, China). The animal experiment was approved by the animal ethics and welfare committee of the Nanjing Medical University (Nanjing, China). The induction of VC in mice with VD3 was performed as we previously described 17 (link). Briefly, mice (n = 10) received intraperitoneal injection with vitamin D3 (350000 IU/kg per day) dissolved in olive oil for 14 consecutive days, and the same volume olive oil injection was used as a control. The body weight of mice was recorded every 3 days during the animal experiment. At day 28 and 42, mouse aortic stiffening and cardiac function were determined under isoflurane anesthesia, respectively. Finally, at day 42, after detection of blood flow of mouse carotid artery in anesthesia, then mouse blood and tissues (aortas, hearts, livers and kidneys) were collected, and the latter were kept in 4% paraformaldehyde solution or snap-frozen in liquid nitrogen.
8
Murine Model of Pharmacological Research
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Male C57BL/6 mice (6–8 weeks old, 18–20 g) were purchased by GemPharmatech LLC. (Nanjing, China; SCXK(SU)2023-0009). All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Jiangsu Province Academy of Traditional Chinese Medicine (Nanjing, China; SYXK(SU)2021-0025).
9
Systematic Mouse Study Protocol
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Male C57BL/6 mice (8 weeks) were purchased from Gempharmatech (Nanjing, Jiangsu, China). Animals were fed with standard food and water in a 12-h light/dark cycle. All the animal protocols and procedures were in accordance with National Institutes of Health guide for the care and use of Laboratory animals (NIH Publications No. 8023, revised 1978) and Vision Research and the Use Committee of Huazhong University of Science and Technology.
10
Vitamin D3 Effect on Mice Aortas
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Male C57BL/6 mice (8 weeks old) were purchased from GemPharmatech (Nanjing, China). The animal experiment was approved by the animal ethics and welfare committee of the Nanjing Medical University (Nanjing, China). Mice (n=10) were intraperitoneally injected with vitamin D3 (400 000 IU/kg per day; Sigma) dissolved in olive oil for 14 consecutive days, and the same volume olive oil injection was used as a control.34 The body weight of mice was recorded every 3 days during the experiment period. At day 28, mice aortas were removed and kept in 4% paraformaldehydesolution for further analysis.
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