Lcms 2010 a spectrometer

All chemicals, reagents, and solvents for the synthesis of indene analogs were purchased from commercial sources and were used without further purification. Indene analogs were synthesized starting from sulindac using a slightly modified protocol as previously described by Bhat et al. [15 (link)]. The reactions were monitored using thin-layer chromatography on silica gel aluminum sheets and visualized with a UV lamp. NMR spectra were recorded on Bruker AV 600 MHz and 300 MHz spectrometers, operating at 150.92 or 75.47 MHz for ¹³C and 600.13 or 300.13 MHz for ¹H nuclei. Chemical shifts are quoted in ppm and are referenced to SiMe4 as an internal standard unless stated otherwise. Multiplets are abbreviated as follows: br—broad; s—singlet; d—doublet; t—triplet; q—quartet; m—multiplet. Mass spectra (ESI-MS) were obtained on Shimadzu LCMS 2010-A spectrometer, Japan. Compounds were purified using column chromatography with silica gel as the stationary phase and ethyl acetate/Petroleum acetate mixtures (5:5) as the eluent system.

¹H-NMR spectra were recorded using TMS as the internal standard in CDCl₃ or dimethyl sulfoxide (DMSO-d₆) solutions on a Bruker 400 MHz instrument (Bruker, Karlsruhe, Germany). MS data were obtained by using a LC-MS-2010A spectrometer (Shimadzu, Kyoto, Japan) with an ESI or ACPI mass selective detector. Elemental analysis was measured by an Elementar Vario EL CHNS Elemental Analyzer (Elementar, Hanau, Germany), and data were uncorrected.