In serum, insulin and glucose levels as well as the lipid profile (triglycerides (TG), cholesterol and HDL) were measured with an automated analyzer (Technicon RA-1000; BayerDiagnostics). VLDL and LDL serum values were estimated as follows: VLDL = TG/5; and LDL = Cholesterol − HDL − VLDL. The HDL/LDL ratio was calculated from these data.
Technicon ra 1000
Manufactured by Bayer
Sourced in United States
The Technicon RA-1000 is a fully automated clinical chemistry analyzer designed for high-throughput sample processing. It is capable of performing a wide range of routine and specialized clinical tests, including those for the assessment of metabolic, renal, and hepatic function. The Technicon RA-1000 is designed to provide accurate and reliable results with a high degree of automation and efficiency.
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Lab products found in correlation
8 protocols using technicon ra 1000
1
Serum Lipid and Glucose Profile
2
Serum Biomarker Analysis Protocol
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The serum levels of Hcy and the transaminases alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured with an automated analyzer (Technicon RA-1000, Bayer Diagnostics, Leverkusen, Germany). Metallopeptidase inhibitor 1 (TIMP-1) was analyzed by the Luminex xMAP (Millipore, Darmstadt, Germany), a system that combines three basic forms of xMAP technology.
3
Plasma and Urine Electrolyte Analysis
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Plasma and urinary samples were diluted 1 : 1 with distilled water and placed into an autoanalyzer (Technicon RA-1000; Bayer, Tarrytown, New York, USA) for sodium, potassium and creatinine determinations.
4
Renal Function Assessment Protocol
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The creatinine, sodium, potassium, and calcium levels in the plasma and urine were measured with an autoanalyzer (Technicon RA-1000, Bayer, Tarrytown, NY and UnicelDxC600 Syncron Clinical Systems. Beckman Coulter, Shelburne, VT). The renal creatinine clearance, absolute sodium excretion, absolute potassium excretion, absolute calcium concentration (29) , and fractional sodium excretion (FENa %) were calculated according to our previous reports (39, (link)40) (link).
The diuretic index is the ratio of urinary volume at each diuretic dose divided by the urinary volume observed in the control group (15) (link).
The diuretic index is the ratio of urinary volume at each diuretic dose divided by the urinary volume observed in the control group (15) (link).
5
Ischemic Reperfusion Injury and Renoprotective Effects
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Example 2
Blood was taken 24 hours after the ischemic reperfusion and the levels of blood urea nitrogen (BUN) and creatine as renal toxicity markers were measured. Renal tissue was taken and prepared into paraffin blocks for immunohistochemical and histological studies. Then, proteins were extracted and the levels of cytokines were measured. The concentrations of creatine and BUN were measured using an autoanalyzer (Technicon RA-1000; Bayer, Tarrytown, N.Y.).
As a result, the PEP 1-administered group showed significantly decreased BUN and creatine levels as compared to the PBS control group (
Flap survivability was measured 7 days after the induction of ischemic reperfusion. The flap survival rate was measured through analysis of digital images using the imageJ program.
As a result, the flap survival rate of the PBS-treated group was 34.69%±16.44% and the PEP1-treated group showed improved flap survival rate of 58.88%±11.44% (see
6
Comprehensive Livestock Biochemical Analysis
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Blood samples were collected in 10-mL tubes containing anticoagulant (EDTA) by passing 3 h from the morning feeding on the day before slaughter. Subsequently, the samples were centrifuged at 6000×rpm for 10 min. For further analyses, these obtained samples were frozen at −20 °C. In order to calculate the concentration of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT), an automatic analyzer (Technicon RA 1000; Bayer Co., NY, USA) was used via proper testing kits (Parsazmun Laboratory, Tehran, Iran). ELISA kit (Stat Fax) and testosterone set (Patangostar-e-Eisar Co, Iran) were used for calculating testosterone concentration. All the studied animals were sacrificed immediately after the end of the experiment and tissue sampling was performed from liver, testis, and humeral muscle tissues (270 samples, including 10 animals × 3 groups × 3 tissues × 3 repeats for each tissue) along with recording weights for the testis, gallbladder, before slaughter, back muscle (loin), warm carcass, lean meat, femur (leg) muscle, and eye muscle area (as described in the study by Masoudzadeh et al., 2020 ).
7
Metabolic Profiling of Dairy Calves
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Blood samples were collected from dairy calves on days 45 and 65, 3 h after morning feeding. The jugular vein was used for blood collection, and K2 EDTA-containing evacuated tubes were used. Samples were immediately placed on ice and centrifuged at 2850×g for 20 min at 4 °C to separate plasma from cells. Then, 1.5 mL of each sample was transferred to 2 mL cryotubes and stored at − 20 °C for subsequent analysis. Plasma concentrations of glucose, urea N, beta-hydroxybutyrate (BHB), and triglycerides were measured using commercial kits (Pars Azmoon Co., Tehran, Iran) and an automated biochemical analyzer (Technicon RA1000; Bayer Corp., Tarrytown, NY, USA) according to the manufacturer's instructions. The results of these measurements were used for further statistical analysis.
8
Chemical Characterization of Animal Feeds
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Subsamples of feeds and refusals were mixed thoroughly and ground to pass a 1-mm screen in a Wiley mill (Ogaw Seiki Co. Ltd., Tokyo, Japan) before chemical analyses for DM content (method 934.01; AOAC, 1990), CP (method 988.05; AOAC, 1990), lipids (AOAC, 1990; method 920.39), calcium (method 985.35; AOAC, 1990) , and phosphorus (method 986.24; AOAC, 1990) . Neutral detergent fiber was analyzed without the use of sodium sulfite and with the inclusion of α-amylase (Van Soest et al., 1991) . Milk samples were analyzed for fat, CP, lactose, and TS content by Milkoscan (Foss Electric, Hillerød, Denmark; AOAC International, 1997).
Plasma glucose concentration [glucose oxidase-phenol 4-aminoantipyrine peroxidase (GOD-PAP) method; Barham and Trinder, 1972] , total protein (Biuret method; Tomas, 1998) , and albumin (bromocresol green method; Johnson et al., 1999) were determined by an automated biochemical analyzer (Technicon RA 1000, Bayer, Tarrytown, NY) using commercial kits (Pars Azmoon Co., Tehran, Iran) according to the manufacturer's instructions. Plasma concentrations of BHBA were determined by enzymatic analysis (BHBA dehydrogenase; kit no. 310, Sigma Chemical; Williamson et al., 1967) .
Plasma glucose concentration [glucose oxidase-phenol 4-aminoantipyrine peroxidase (GOD-PAP) method; Barham and Trinder, 1972] , total protein (Biuret method; Tomas, 1998) , and albumin (bromocresol green method; Johnson et al., 1999) were determined by an automated biochemical analyzer (Technicon RA 1000, Bayer, Tarrytown, NY) using commercial kits (Pars Azmoon Co., Tehran, Iran) according to the manufacturer's instructions. Plasma concentrations of BHBA were determined by enzymatic analysis (BHBA dehydrogenase; kit no. 310, Sigma Chemical; Williamson et al., 1967) .
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