Uc10 4b9

Manufactured by Thermo Fisher Scientific

The UC10-4B9 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate up to 4 tubes with a maximum capacity of 10 mL each. The centrifuge is capable of reaching a maximum speed of 4,000 rpm, providing sufficient force for a wide range of sample separation and preparation tasks.

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Lab products found in correlation

Rm4 5, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Ra3 6b2, by Thermo Fisher Scientific (1 mentions) Mel 14, by BioLegend (1 mentions) α cd8 5h10, by Thermo Fisher Scientific (1 mentions) Af6 120, by Thermo Fisher Scientific (1 mentions) Facscanto 2 cytometer, by BD (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Fjk 16s, by BD (1 mentions) Recombinant mouse il 2, by R&D Systems (1 mentions) F4 80 bm8, by Thermo Fisher Scientific (1 mentions) Il 33, by R&D Systems (1 mentions) Interleukin 7 (il 7), by R&D Systems (1 mentions) Sema3a fc, by R&D Systems (1 mentions)

Spelling variants of this product

CTLA-4 (UC10-4B9), (5 citations) Anti-mouse CTLA-4 (UC10-4B9; (3 citations) Anti-CTLA-4 (UC10-4B9). (2 citations) CTLA-4 (clone UC10-4B9, (2 citations) Anti-CTLA-4-PE (clone UC10-4B9), (2 citations) Anti-CTLA-4 PE-eFluor610 (clone UC10-4B9; (2 citations) Anti-CTLA-4-PE (UC10-4B9) (1 citations)

3 protocols using uc10 4b9

Comprehensive Phenotyping of Mouse Splenocytes

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2 × 10⁶ freshly isolated mouse splenocytes, resuspended in PBS with 2% FBS, were labeled for 30 min at 4°C in the dark with different antibody panels. The antibodies used were: α-CD8 (5H10, Thermo Fisher, cat. nr. MCD0828TR), α-CD4 (RM4-5, Thermo Fisher, cat. nr. 56-0042-80), α-CD3 (17A2, eBioscience, cat. nr. 46-0032-80), α-CD62L (MEL-14, Biolegend, cat. nr. 104411), α-CD44 (IM7, Biolegend, cat. nr. 103005), α-PD-1 (29F-1A12, Biolegend, cat. nr. 135223), α-IL7R (A7R34, Biolegend, cat. nr. 135031), α-CTLA-4 (UC10-4B9, eBioscience, cat. nr. 12-1522-81), α-B220 (RA3-6B2, eBioscience, cat. nr. 47-0452-82), α-CD21 (eBio4E3, eBioscience, cat. nr. 48-0212-80), α-CD23 (B3B4, Biolegend, cat. nr. 101612), α-CD86 (GL1, eBioscience, cat. nr. 12-0862-81), α-CD69 (H1.2F3, eBioscience, cat. nr. 11-0691-81), α-IAb (AF6-120.1, eBioscience, cat. nr. 46-5320-80). The cells were then washed twice with PBS, resuspended in PBS with 2% FBS and data were acquired on an LSRII Flow Cytometer (BD Biosciences) and analyzed with FlowJo software, ver. 9.9.6 (Treestar, Inc).

Fenninger F., Han J., Stanwood S.R., Nohara L.L., Arora H., Choi K.B., Munro L., Pfeifer C.G., Shanina I., Horwitz M.S, & Jefferies W.A. (2019). Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction. Frontiers in Immunology, 10, 2473.

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Identifying Regulatory T Cells in Pancreas

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Flow cytometry was performed on a FACSCanto II cytometer (BD Biosciences) using antibodies against Foxp3 (eBioscience, FJK-16s), CD4 (BD Biosciences, RM4-5), and CTLA-4 (eBioscience, UC10-4B9). LIVE/DEAD Fixable Dead Cell Stain Kit (Life Technologies) was used to exclude dead cells. Cells were isolated from the pancreas by digestion with collagenase P. Collected data were analyzed using FlowJo software (Tree Star).

Bengsch F., Knoblock D.M., Liu A., McAllister F, & Beatty G.L. (2017). CTLA-4/CD80 pathway regulates T cell infiltration into pancreatic cancer. Cancer immunology, immunotherapy : CII, 66(12), 1609-1617.

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Flow Cytometry Antibody Panel for Murine Immunology

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mAbs specific for mouse CD3ε (145-2C11), CD4 (GK1.5), CD5 (53-7.3), CD8a (53-6.7), CD11c (HL3), CD16/CD32 (2.4G2), CD19 (1D3), CD25 (PC61), Gr-1 (RB6-8C5), NK1.1 (PK136), Siglec-F (E50-2440), ST2 (U29-93), and Thy1.2 (53-2.1) were purchased from BD Biosciences. mAbs specific for mouse α4β7 (DATK32), CD11b (M1/70), CD31 (MEC13.3), CD45 (30F11), CD45.1 (A20), CD45.2 (104), CD127 (A7R34), EpCAM (G8.8), erythroid cell marker (TER-119; TER-119), FcγRIα (MAR-1), Flt3 (A2F10), Ki67 (16A8), PDGFRα (APA5), PD-1 (29F.1A12), Sca-1 (D7), TIGIT (1G9), IL-5 (TRFK5), and fluorochrome-conjugated streptavidin were purchased from BioLegend. mAbs specific for mouse CD25 (PC61.5), CTLA4 (UC10-4B9), IL-13 (eBio13A), KLRG1 (2F1), and F4/80 (BM8) were purchased from eBioscience.
Recombinant mouse IL-2, IL-7, IL-33, Sema3A-Fc, and Sema6D-Fc were purchased from R&D Systems. Anti-IL-10 mAb (JES5-2A5) was purchased from Bio X cell.

Naito M., Nakanishi Y., Motomura Y., Takamatsu H., Koyama S., Nishide M., Naito Y., Izumi M., Mizuno Y., Yamaguchi Y., Nojima S., Okuzaki D, & Kumanogoh A. (2022). Semaphorin 6D–expressing mesenchymal cells regulate IL-10 production by ILC2s in the lung. Life Science Alliance, 5(11), e202201486.

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