Echimidine (94% purity), heliotrine (91% purity), lasiocarpine (98% purity), senecionine (99% purity) and senkirkine (98% purity) were purchased from PhytoLab (Verstenbergsgreuth, Germany). Platyphylline (95% purity) was purchased from BOC Sciences (New York, New York, USA). senecionine was dissolved in 0.2-M hydrochloric acid. Afterwards, pH was adjusted to 6–7 using 0.2-M sodium hydroxide solution. Sodium chloride (NaCl) was added to obtain a solution containing 0.15-M NaCl. All other PA were dissolved in 0.15-M aqueous NaCl solution.
Platyphylline
Manufactured by BOC Sciences
Sourced in United States
Platyphylline is a laboratory compound available from BOC Sciences. It is a natural plant-derived substance used for research purposes. The core function of Platyphylline is to serve as a reference standard or analytical tool in scientific investigations.
Automatically generated - may contain errors
4 protocols using platyphylline
1
Extraction and Preparation of Pyrrolizidine Alkaloids
2
Structural Diversity of Pyrrolizidine Alkaloids
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The structurally different PAs echimidine (94% purity), heliotrine (91% purity), lasiocarpine (98% purity), senecionine (99% purity), and senkirkine (98% purity) were purchased from PhytoLab (Verstenbergsgreuth, Germany). Platyphylline (95% purity) was purchased from BOC Sciences (New York, USA). PAs were dissolved in 0.15 M NaCl. Due to the poorer solubility, senecionine was first dissolved in 0.2 M hydrochloric acid, then the pH was adjusted to 6–7 and afterwards NaCl was added to a final concentration of 0.15 M.
3
Pyrrolizidine Alkaloids Analysis Protocol
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All PAs used in this study were purchased from Phytoplan Diehm & Neuberger GmbH (Heidelberg, Germany), except platyphylline which was obtained from BOC Sciences (New York, NY, USA). The purity of all PAs was at least 95%. PAs were stored at −20 °C dissolved in 50% Acetonitrile (ACN)/50% H2O as 5 mM stock solutions. All other chemicals were obtained from Merck (Darmstadt, Germany) or Sigma-Aldrich (Taufkirchen, Germany) in the highest purity available.
4
Purity and Preparation of Plant Alkaloids
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The PAs used in this study, except platyphylline, were purchased from Phytoplan Diehm & Neuberger GmbH (Heidelberg, Germany) with a purity of at least 95%. platyphylline was obtained from BOC Sciences (New York, NY, USA). All PAs were dissolved in 50% acetonitrile (ACN, Sigma-Aldrich, Taufkirchen, Germany)/50% H2O. The 5 mM stock solutions were stored at −20 °C. All other chemicals used in this study were obtained from Merck (Darmstadt, Germany) or Sigma-Aldrich (Taufkirchen, Germany) in the highest available purity.
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