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Rabbit anti cleaved caspase 3 9661

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Rabbit anti-cleaved caspase-3 (9661) is a primary antibody directed against the cleaved form of caspase-3. Caspase-3 is a key effector caspase that plays a central role in the execution-phase of cell apoptosis.

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Lab products found in correlation

Mouse anti e cadherin, by BD (1 mentions) Sc 15334, by Santa Cruz Biotechnology (1 mentions) Cy2 and cy3 conjugated fluorescent secondary antibodies, by Jackson ImmunoResearch (1 mentions) Biotinylated secondary antibodies for immunohistochemical assays, by Vector Laboratories (1 mentions) Rabbit anti lysozyme, by Agilent Technologies (1 mentions) Pk 6100, by Vector Laboratories (1 mentions) Gfp 1020, by Aves Labs (1 mentions) Mouse anti β catenin, by BD (1 mentions) Mouse anti gli1 l42b10, by Cell Signaling Technology (1 mentions) Rabbit anti pax6 prb 278p, by BioLegend (1 mentions) Westernbright ecl hrp substrate, by Advansta (1 mentions) P raptor, by Cell Signaling Technology (1 mentions) Rat anti mouse f4 80, by Bio-Rad (1 mentions) Rabbit anti mouse cox2, by Cayman Chemical (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions) 2 4 amidinophenyl 6 indolecarbamidine dihydrochloride dapi, by Merck Group (1 mentions) Z vad fmk, by Beyotime (1 mentions) N acetylcysteine nac, by Merck Group (1 mentions) Mouse anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)

5 protocols using rabbit anti cleaved caspase 3 9661

1

Antibody Characterization for Immunostaining

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The following antibodies were used for immunofluorescent or immunohistochemical analysis: rabbit anti-Ki67 (VP-K451, 1:1000; Vector Laboratories; Burlingame, CA), rabbit anti-lysozyme (A0099, 1:1000; Dako; Glostrup Municipality, Denmark), mouse anti–E-cadherin (610181, 1:500; BD Transduction Laboratories; San Jose, CA), chicken anti-green fluorescent protein (GFP) for the detection of enhanced yellow fluorescent protein (EYFP) (GFP-1020, 1:2000; Aves Labs; Tigard, OR), rabbit anti-cyclin D1 (CRM 307, 1:100; Biocare Medical; Concord, CA), rabbit anti-cleaved caspase-3 (9661, 1:200; Cell Signaling Technology; Danvers, MA), mouse anti–β-catenin (610153, 1:200; BD Biosciences, San Jose, CA), and rabbit anti–mucin 2 (sc-15334, 1:1000; Santa Cruz Biotechnology; Dallas, TX). Cy2- and Cy3-conjugated fluorescent secondary antibodies were purchased from The Jackson Laboratory and used at a 1:200 dilution. Biotinylated secondary antibodies for immunohistochemical assays (Vector Laboratories) were used at a 1:200 dilution along with the ABC detection system (PK-6100; Vector Laboratories).
Kieckhaefer J.E., Lukovac S., Ye D.Z., Lee D., Beetler D.J., Pack M, & Kaestner K.H. (2016). The RNA Polymerase III Subunit Polr3b Is Required for the Maintenance of Small Intestinal Crypts in Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(6), 783-795.
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2

Western Blot Analysis of Protein Expression

Cited 2 times
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Total protein extracts, SDS-PAGE separation and Western Blot were performed with standard methods as described elsewhere57 (link),58 (link). Antibodies were as follows: goat anti-β-actin #SC-1616, mouse anti-α Tubulin TU-02 #SC-8035, rabbit anti-CCND1 #SC-717 and mouse anti-MYCN #SC-53993, mouse anti-Vinculin #SC-73614, mouse anti-Math1 (DSHB), goat anti-DCX # SC-8066 (Santa Cruz Biotechnology); rabbit anti-PARP1 #9542, mouse anti-Gli1 #L42B10, rabbit anti-cleaved caspase-3 #9661 (Cell Signaling Technology Inc); rabbit anti-Myc #C3956 (Sigma Aldrich); rabbit anti-ZIC1 ab72694 (ABCAM), rabbit anti-Pax6 #PRB-278P (BioLegend), mouse anti-Nestin ab11306 (ABCAM). All antibodies were previously used in56 (link),59 (link)–63 (link). Immunoreactive bands were visualized by enhanced chemoluminscence using WesternBright ECL HRP substrate (Advansta).
Petroni M., Sahùn Roncero M., Ramponi V., Fabretti F., Nicolis Di Robilant V., Moretti M., Alfano V., Corsi A., De Panfilis S., Giubettini M., Di Giulio S., Capalbo C., Belardinilli F., Coppa A., Sardina F., Colicchia V., Pedretti F., Infante P., Cardinali B., Tessitore A., Canettieri G., De Smaele E, & Giannini G. (2019). SMO-M2 mutation does not support cell-autonomous Hedgehog activity in cerebellar granule cell precursors. Scientific Reports, 9, 19623.
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3

Immunohistochemical and Immunoblotting Analyses of Inflammatory Signaling Pathways

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The primary antibodies that were used for immunohistochemistry and immunoblotting included rabbit anti-mouse COX-2 from Cayman Chemicals, cyclin D1 and c-Myc from Santa Cruz Biotechnology; rat anti-mouse F4/80 (marker of macrophages/dendritic cells) from AbD Serotec; rabbit anti-p-ERK, p-p70 S6K (Thr389), p-PI3K p85 (Tyr458), p-PDK1 (Ser241), p-AKT (Thr308), p-mTOR (Ser2448), p-raptor (Ser792), p-elF-4B(Ser422), p-S6 ribosomal protein (p-rpS6, Ser240/244), and rabbit anti-cleaved caspase-3 (9661) from Cell Signaling Technology; rabbit anti-iNOS, rabbit-anti-Ki67 (ab15580), and goat anti-arginase 1 from Abcam, mouse anti-interleukin 4 receptor α (IL-4Rα) and mannose receptor (MR, CD206) from R&D.
Chang J., Vacher J., Yao B., Fan X., Zhang B., Harris R.C, & Zhang M.Z. (2015). Prostaglandin E receptor 4 (EP4) promotes colonic tumorigenesis. Oncotarget, 6(32), 33500-33511.
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4

Cytotoxicity Assays and Apoptosis Markers

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The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; 98 % purity), 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI; purity by HPLC: ≥ 98 %), 5-FU (purity by HPLC: ≥ 99 %), and N-acetylcysteine (NAC; purity by TLC: ≥ 99 %) were purchased from Sigma-Aldrich (St Louis, MO, USA). Mito-Tracker Green and Z-VAD-FMK were purchased from Beyotime (Shanghai, China). Mouse anti-PCNA (60097) was purchased from Proteintech (Chicago, IL, USA). Mouse anti-p53 (ab26) and the rabbit anti-cytochrome c (ab133504) were purchased from Abcam (Cambridge, MA, USA). Rabbit anti-Bcl-2 (sc-492), rabbit anti-Bax (sc-526) and mouse anti-β-actin (sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-cleaved caspase-3 (9661) was obtained from Cell Signaling Technology (Beverly, MA, USA).
Hu Z., Lv G., Li Y., Li E., Li H., Zhou Q., Yang B, & Cao W. (2016). Enhancement of anti-tumor effects of 5-fluorouracil on hepatocellular carcinoma by low-intensity ultrasound. Journal of Experimental & Clinical Cancer Research : CR, 35, 71.
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5

Immunoblotting for Apoptosis Markers

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Proteins were extracted from cells using RIPA buffer (Nacalai Tesque, Kyoto, Japan) containing cOmplete ™ Protease Inhibitor Cocktail (Sigma-Aldrich, St. Louis, MO, USA) and the protein concentration was adjusted to 1 mg/mL. Immunoblotting was performed as previously described [16 (link)]. The primary antibodies used in the experiments were as follows: rabbit anti-PAFR, # bs-14730R-A750 (Bioss. Inc., Woburn, MA, USA), 1:200; rabbit anti-Erk1/2 # 9102 (Cell Signaling Technology, Beverly, MA, USA), 1:1000; rabbit anti-p-Erk1/2 # 4370 (Cell Signaling Technology), 1:1000; rabbit anti-Akt # 4691 (Cell Signaling Technology), 1:1000; rabbit anti-p-Akt #4060 (Cell Signaling Technology), 1:1000; rabbit anti-Caspase-3 #9662 (Cell Signaling Technology), 1:1000; rabbit anti-Cleaved caspase-3 #9661 (Cell Signaling Technology), 1:1000; and mouse anti-α tubulin # sc-5286 (Santa Cruz Biotechnology, Shanghai, China).
Kawasaki K., Kasamatsu A., Ando T., Saito T., Nobuchi T., Nozaki R., Iyoda M, & Uzawa K. (2021). Ginkgolide B Regulates CDDP Chemoresistance in Oral Cancer via the Platelet-Activating Factor Receptor Pathway. Cancers, 13(24), 6299.
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