wg-Gal4 transgenic flies were generated by integrating the Gal4 containing plasmid RIVGal4 (Baena-Lopez et al., 2013 (link)) into the attP site of wg flies (Alexandre et al., 2014 (link)).
Uas arm s10
The UAS-Arm-S10 is a robotic arm designed for laboratory applications. It features 6 degrees of freedom and a payload capacity of up to 10 kilograms. The arm is controlled via a software interface and can be programmed to perform a variety of tasks within a laboratory setting.
2 protocols using uas arm s10
Genetic Manipulation of Drosophila Development
wg-Gal4 transgenic flies were generated by integrating the Gal4 containing plasmid RIVGal4 (Baena-Lopez et al., 2013 (link)) into the attP site of wg flies (Alexandre et al., 2014 (link)).
Drosophila ago RNAi and Overexpression
All Gal4 lines were obtained from BDSC except ci-Gal4 (gift from R. A. Holmgren). wg-LacZ [37 (link)], tub-Gal80ts (BDSC#7017), UAS-wg RNAi (NIG#4889R-3), UAS-armS10 (BDSC#4782), UAS-cyc E (BDSC#4781), UAS-dmyc (BDSC#9674), UAS-nuclear lacZ (BDSC#3955) and UAS-GFP (BDSC#1522) lines were also used. All cultures were carried out at either 18 °C or 25 °C. For conditional induction of Gal4, flies with tub-Gal80ts were incubated at 18 °C until induction at 29 °C.
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