The largest database of trusted experimental protocols

Uas arm s10

Manufactured by BD

The UAS-Arm-S10 is a robotic arm designed for laboratory applications. It features 6 degrees of freedom and a payload capacity of up to 10 kilograms. The arm is controlled via a software interface and can be programmed to perform a variety of tasks within a laboratory setting.

Automatically generated - may contain errors

2 protocols using uas arm s10

1

Genetic Manipulation of Drosophila Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly culcture and crosses were performed according to standard procedures. Drosophila stocks used in this study include: UAS-Cut (#36496; Bloomington stock center (BDSC)); FRT19A, cutC145 (#36496; BDSC); UAS-Wg-HA (#5918; BDSC); UAS-Arm-S10 (#4782; BDSC); UAS-cut-RNAi (#33967; BDSC); UAS-br-RNAi (#27272; BDSC); esg-Gal4, tubP-Gal80ts, UAS-GFP (Biteau et al., 2008 (link); Micchelli and Perrimon, 2006 (link)); wg-Gal4 (Alexandre et al., 2014 (link)); wg(KO; NRT–Wg) (Alexandre et al., 2014 (link)); UAS-TCF-ΔN (#4785; BDSC); wg-lacZ (#1672; BDSC); Rab3-GFP (#62541; BDSC); UAS-CD8-RFP (#27392, BDSC); cut-intron2-Gal4 (#49818, BDSC); NRE-GFP (#30727; BDSC); UAS-Sgg.S9A (#5255; BDSC); UAS-EcR-DN (#6872; BDSC); UAS-wg-RNAi (#33902; BDSC); UAS-Br-Z1 (#51190; BDSC); UAS-Br-Z3 (#51192; BDSC); UAS-Br-Z4 (#51193; BDSC); FRT19A, dsh3 (#6331; BDSC); UAS-TCF (#4838; BDSC); UAS-Nipped-B-RNAi (#32406; BDSC); UAS-SA-RNAi (#33395; BDSC); UAS-Eip74EF-RNAi (#29353; BDSC); UAS-Eip75B (#26717; BDSC); UAS-white-RNAi (#33623; BDSC); Act5C-FRT>stop>FRT-lacZ.nls (#6355; BDSC).
wg-Gal4 transgenic flies were generated by integrating the Gal4 containing plasmid RIVGal4 (Baena-Lopez et al., 2013 (link)) into the attP site of wg flies (Alexandre et al., 2014 (link)).
+ Open protocol
+ Expand
2

Drosophila ago RNAi and Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two ago RNAi lines, #31501 and #34802, were obtained from Bloomington Drosophila stock center (BDSC). The targeting regions for BDSC#31501 and #34802 are 2504-2913th and 5538-5558th nucleotide, respectively (Additional file: Fig. S1 a). Two ago overexpression fly lines used are: FlyORF library #F001828 and line generated with pUAS-myc-ago construct from our laboratory. To make pUAS-myc-ago, an ago cDNA clone (Drosophila Genomics Resource Center clone #LD30271) was sequenced and cloned into pUAST-myc.
All Gal4 lines were obtained from BDSC except ci-Gal4 (gift from R. A. Holmgren). wg-LacZ [37 (link)], tub-Gal80ts (BDSC#7017), UAS-wg RNAi (NIG#4889R-3), UAS-armS10 (BDSC#4782), UAS-cyc E (BDSC#4781), UAS-dmyc (BDSC#9674), UAS-nuclear lacZ (BDSC#3955) and UAS-GFP (BDSC#1522) lines were also used. All cultures were carried out at either 18 °C or 25 °C. For conditional induction of Gal4, flies with tub-Gal80ts were incubated at 18 °C until induction at 29 °C.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!