Log In Sign up
The largest database of trusted experimental protocols

Pqxip

Manufactured by Takara Bio
Visit Supplier

The PQXIP is a laboratory equipment product developed by Takara Bio. It is a device designed for performing nucleic acid extraction and purification. The core function of the PQXIP is to automate the process of extracting and purifying DNA or RNA samples from various biological sources.

Automatically generated - may contain errors

Lab products found in correlation

Pcdna dest40, by Thermo Fisher Scientific (1 mentions) V5 tag, by Thermo Fisher Scientific (1 mentions)

3 protocols using pqxip

1

Cloning and Tagging of RARA and RXRB

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wild-type RARA and RXRB entry clones were obtained from the hORFeome v8.171 (link) collection. Gateway LR reactions were used to transfer bait RARA wild-type, p.Pro375Leu, and p.Arg83His into a pQXIP (ClonTech, 631516) vector modified to include a Gateway cassette featuring a C-terminal 3×FLAG. Prey RXRB was transferred into pcDNA-DEST40 which includes a V5 tag (Invitrogen, 12274–015) also using Gateway LR reactions.
Chen S., Fragoza R., Klei L., Liu Y., Wang J., Roeder K., Devlin B, & Yu H. (2018). An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. Nature genetics, 50(7), 1032-1040.
+ Open protocol
+ Expand
2

SARS-CoV-1 and SARS-CoV-2 Protein Interaction Mapping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Clones of all human proteins tested were picked from the hORFeome 8.1 library121 (link). Clones for all SARS-CoV-1 and SARS-CoV-2 proteins tested were designed to match GenBank entries AY357076 and MN908947 respectively. To construct plasmids for testing by Y2H viral genes were PCR amplified and cloned into PDEST-AD and PDEST-DB vectors (for Y2H). For co-immunoprecipitation (co-IP) Gateway LR reactions were used to transfer bait SARS-CoV-2 nsp1 protein into a pQXIP (ClonTech, 631516) vector modified to include a Gateway cassette featuring a carboxy-terminal 3×FLAG.
Wierbowski S.D., Liang S., Liu Y., Chen Y., Gupta S., Andre N.M., Lipkin S.M., Whittaker G.R, & Yu H. (2021). A 3D Structural SARS-CoV-2-Human Interactome to Explore Genetic and Drug Perturbations. Nature methods, 18(12), 1477-1488.
+ Open protocol
+ Expand
3

SARS-CoV-1 and SARS-CoV-2 Protein Interaction Mapping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Clones of all human proteins tested were picked from the hORFeome 8.1 library121 (link). Clones for all SARS-CoV-1 and SARS-CoV-2 proteins tested were designed to match GenBank entries AY357076 and MN908947 respectively. To construct plasmids for testing by Y2H viral genes were PCR amplified and cloned into PDEST-AD and PDEST-DB vectors (for Y2H). For co-immunoprecipitation (co-IP) Gateway LR reactions were used to transfer bait SARS-CoV-2 nsp1 protein into a pQXIP (ClonTech, 631516) vector modified to include a Gateway cassette featuring a carboxy-terminal 3×FLAG.
Wierbowski S.D., Liang S., Liu Y., Chen Y., Gupta S., Andre N.M., Lipkin S.M., Whittaker G.R, & Yu H. (2021). A 3D Structural SARS-CoV-2-Human Interactome to Explore Genetic and Drug Perturbations. Nature methods, 18(12), 1477-1488.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!