To assess the mRNA complexation ability of G0-C14 and its stability in organic solvent (DMF), naked EGFP-mRNA or EGFP-mRNA complexed with G0-C14 (in varying weight ratios from 1 to 20) were incubated with or without DMF for 30 min. For mRNA samples in DMF, electrophoresis was run without extracting mRNA from DMF into aqueous solution. The volumes of samples were then adjusted with loading dye (Invitrogen) and run into an E-Gel® 2% agarose (Invitrogen) gel for 30 min at 50 V. The ambion® millennium™ markers-formamide (Thermo Fisher Scientific) was used as a ladder. Finally, the gel was imaged under UV and the bands were analyzed.
Ambion millennium markers formamide
Manufactured by Thermo Fisher Scientific
Ambion® millennium™ markers-formamide are molecular weight markers used in nucleic acid electrophoresis applications. They provide a reference for determining the size of DNA or RNA fragments separated on a gel.
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Lab products found in correlation
3 protocols using ambion millennium markers formamide
1
mRNA Complexation and Stability in DMF
2
Optimizing mRNA Delivery via G0-C8
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Gel electrophoresis was used to study the mRNA complexation ability of ionizable compound G0-C8 and optimize the ratio between G0-C8 and mRNA in the NPs with free EGFP-mRNA or EGFP-mRNA complexed with G0-C8. Free EGFP-mRNA was also incubated with DMF to evaluate the stability of mRNA in organic solvent (DMF). The EGFP-mRNA were first incubated with G0-C8 at different weight ratios (weight ratios of G0-C8/mRNA: 1, 2, 5, 10, and 20) or DMF for 20 min at room temperature. The volumes of samples were then adjusted with loading dye (Invitrogen) and run into an E-Gel 2% agarose (Invitrogen) gel for 30 min at 50 V. Ambion Millennium markers-Formamide (Thermo Fisher Scientific) was used as a ladder. Finally, the gel was imaged under ultraviolet and the bands were analyzed.
3
mRNA Complexation and Stability in DMF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the mRNA complexation ability of G0-C14 and its stability in organic solvent (DMF), naked EGFP-mRNA or EGFP-mRNA complexed with G0-C14 (in varying weight ratios from 1 to 20) were incubated with or without DMF for 30 min. For mRNA samples in DMF, electrophoresis was run without extracting mRNA from DMF into aqueous solution. The volumes of samples were then adjusted with loading dye (Invitrogen) and run into an E-Gel® 2% agarose (Invitrogen) gel for 30 min at 50 V. The ambion® millennium™ markers-formamide (Thermo Fisher Scientific) was used as a ladder. Finally, the gel was imaged under UV and the bands were analyzed.
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