All images were obtained using a Leica DM4000 B upright fluorescent microscope (Wetzlar, Germany) equipped with a Leica EL6000 light source and a Retiga 4000R camera (Qimaging, Surrey, Canada) running Image-Pro Plus software version 6.3.0.512 (Media Cybernetics, Rockville, USA). Human neutrophil images were captured at a magnification of 200× and murine neutrophils images were captured at 400×. Five images were taken per coverslip at standardized locations. If no cells were visible within one of the locations, additional images were taken at points exactly between the standard five locations and labeled accordingly.
Dm4000 b upright fluorescent microscope
Manufactured by Leica
Sourced in Germany
The Leica DM4000 B is an upright fluorescent microscope designed for advanced imaging and analysis. It features a modular design, allowing for customization to suit various research and laboratory applications. The microscope provides high-performance optics and illumination systems to capture detailed fluorescent images.
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Lab products found in correlation
2 protocols using dm4000 b upright fluorescent microscope
1
Fluorescence Microscopy Imaging of Human and Murine Neutrophils
2
Fluorescence Microscopy Data Analysis
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Slides were examined and imaged with a Leica DM4000B upright fluorescent microscope (Wetzlar, Germany) or a Leica SP5 confocal imaging system. For multiple comparisons, a 1-way analysis of variance (ANOVA) followed by Tukey test was used. For single comparison, a 2-tailed unpaired Student's t-test was used.
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