Fitc conjugatedanti mouse cd19 clone 1d3

Manufactured by BD

Sourced in United States

FITC-conjugated anti-mouse CD19 (clone 1D3) is a laboratory reagent used to detect and study mouse CD19 protein expression. CD19 is a cell surface marker expressed on B cells. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and quantification of CD19-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Facs canto 2 instrument, by BD (1 mentions) Facsdiva version 6, by BD (1 mentions) Cellquest software, by BD (1 mentions)

Spelling variants of this product

CD19-FITC (75 citations) CD19 (1D3, (23 citations) CD19 (clone 1D3, (15 citations) CD19 FITC (clone 1D3, (5 citations) Clone 1D3 (5 citations) CD19 FITC (1D3), (4 citations)

2 protocols using fitc conjugatedanti mouse cd19 clone 1d3

Multiparametric Flow Cytometry of Immune Cells

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Isolated cells (1–2 × 10⁶) were stained for flow
cytometry analysis using the following antibodies: FITC-conjugated
anti-mouse CD19 (clone 1D3) (BD Biosciences, San Diego, CA, USA),
FITC-conjugated anti-mouse CD4 (clone GK1.5) and IgM (clone RMM-1)
(BioLegend, San Diego, CA, USA), FITC-conjugated anti-mouse CD11c
(clone N418), (eBioscience, San Diego, CA, USA), PE-conjugated
anti-mouse CD8a (clone 53-6.7) and CD49b (clone DX5) (eBiosciences),
PE-conjugated anti-mouse IgA (clone 11-44-2) and MHC class II (clone
NIMR-4) (eBioscience), and PE-Cy5-conjugated anti-mouse CD3e (clone
145-2C11) (eBioscience). Stained samples were analyzed using a BD
FACSCanto II instrument and the BD FACSDiva version 6.1.3 software
(BD Biosciences).

KISHINO E., TAKEMURA N., MASAKI H., ITO T, & NAKAZAWA M. (2015). Dietary lactosucrose suppresses influenza A (H1N1) virus infection in mice. Bioscience of Microbiota, Food and Health, 34(4), 67-76.

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Multicolor Flow Cytometry for Immune Cell Phenotyping

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All monoclonal antibodies were purchased from BD Biosciences (San Jose, CA, USA) or eBioscience (San Diego, CA, USA). Lymphocytes harvested from the spleens were stained with different marker-specific antibodies: allophycocyanin-conjugated anti-mouse CD4 (clone GK1.5), phycoerythrin-conjugated anti-mouse CD8a (clone 53–6.7), or FITC-conjugated anti-mouse CD19 (clone 1D3; BD Biosciences Pharmingen). The stained cells were analyzed with a FACSCalibur cell sorter using CellQuest software (Becton Dickinson, San Jose, CA, USA).

Wu C.C., Huang Y.S., Chen J.S., Huang C.F., Su S.L., Lu K.C., Lin Y.F., Chu P., Lin S.H, & Sytwu H.K. (2015). Resveratrol Ameliorates Renal Damage, Increases Expression of Heme Oxygenase-1, and Has Anti-Complement, Anti-Oxidative, and Anti-Apoptotic Effects in a Murine Model of Membranous Nephropathy. PLoS ONE, 10(5), e0125726.

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