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Psmd1 c 7

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PSMD1 (C-7) is a mouse monoclonal antibody that recognizes the PSMD1 (26S Proteasome Non-ATPase Regulatory Subunit 1) protein. PSMD1 is a component of the 26S proteasome, which is responsible for the degradation of ubiquitinated proteins in eukaryotic cells. The antibody can be used for the detection of PSMD1 in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Alpha tubulin ab80779, by Abcam (2 mentions) Psmc2 mss1 104, by Enzo Life Sciences (2 mentions) Np0009, by Thermo Fisher Scientific (2 mentions) Beta actin n 21, by Santa Cruz Biotechnology (2 mentions) Erα f 10, by Santa Cruz Biotechnology (2 mentions) Odyssey clx imaging machine, by LI COR (2 mentions) Image studio software, by LI COR (2 mentions) Image lab software, by Bio-Rad (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Chemidoc mp system, by Bio-Rad (2 mentions)

2 protocols using psmd1 c 7

1

Quantitative Immunoblotting of PSMC2, PSMD2, and ERα

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For PSMC2 and PSMD2 immunoblotting, cells were lysed in HENG buffer (50mM Hepes-KOH pH 7.9, 150mM NaCl, 2mM EDTA pH 8.0, 20mM sodium molybdate, 0.5% Triton X-100, 5% glycerol), with protease inhibitor cocktail (Roche Diagnostics #11836153001). Protein concentration was determined by the BCA assay (Thermo-Fisher Scientific #23227), and proteins were resolved on SDS-PAGE for immunoblot analysis. Antibodies against the following human proteins were used: alpha-Tubulin (ab80779; Abcam), PSMC2 (MSS1–104; Enzo Life Sciences) and PSMD1 (C-7; Santa-Cruz). Visualization was performed using the ChemiDoc MP System (Bio-Rad), and ImageLab Software (Bio-Rad) was used to quantify relative band intensities. For ERα immunonblotting, cells were lysed with a mix of 4X protein loading buffer (Li-Cor 928–40004) and 10X NuPAGE sample reducing agent (Life Technologies NP0009). Protein concentration was normalized by cell counting, and proteins were resolved on SDS-PAGE. Antibodies against the following human proteins were used: beta-Actin (N-21; Santa Cruz), ERα (F-10; Santa Cruz). Visualization was performed using the Odyssey CLx imaging machine (Li-Cor), and Image Studio Software (Li-Cor) was used to quantify the relative intensities.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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2

Quantitative Immunoblotting of PSMC2, PSMD2, and ERα

Check if the same lab product or an alternative is used in the 5 most similar protocols
For PSMC2 and PSMD2 immunoblotting, cells were lysed in HENG buffer (50mM Hepes-KOH pH 7.9, 150mM NaCl, 2mM EDTA pH 8.0, 20mM sodium molybdate, 0.5% Triton X-100, 5% glycerol), with protease inhibitor cocktail (Roche Diagnostics #11836153001). Protein concentration was determined by the BCA assay (Thermo-Fisher Scientific #23227), and proteins were resolved on SDS-PAGE for immunoblot analysis. Antibodies against the following human proteins were used: alpha-Tubulin (ab80779; Abcam), PSMC2 (MSS1–104; Enzo Life Sciences) and PSMD1 (C-7; Santa-Cruz). Visualization was performed using the ChemiDoc MP System (Bio-Rad), and ImageLab Software (Bio-Rad) was used to quantify relative band intensities. For ERα immunonblotting, cells were lysed with a mix of 4X protein loading buffer (Li-Cor 928–40004) and 10X NuPAGE sample reducing agent (Life Technologies NP0009). Protein concentration was normalized by cell counting, and proteins were resolved on SDS-PAGE. Antibodies against the following human proteins were used: beta-Actin (N-21; Santa Cruz), ERα (F-10; Santa Cruz). Visualization was performed using the Odyssey CLx imaging machine (Li-Cor), and Image Studio Software (Li-Cor) was used to quantify the relative intensities.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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