Anti ctbp2 ribeye

Immunohistochemistry: anti-RBP2 (guinea pig polyclonal, 1:1000; kind gift of Eckart Gundelfinger; specifically recognizes RBP2 as verified by the knockout, [21 (link)]); anti-Cav1.3 (rabbit polyclonal, 1:500; Alomone Labs; [53 (link)]); anti-CtBP2/RIBEYE (mouse monoclonal, 1:200; BD Biosciences); Alexa488 anti-guinea pig (goat polyclonal; 1:500; Invitrogen), Alexa568 anti-mouse (goat polyclonal; 1.500; Invitrogen), Cy3 anti-rabbit (donkey polyclonal; 1:1500; Jackson Immuno Research); co-immunoprecipitation: affinity purified anti-RBP2-1318 (rabbit polyclonal; 1:100; directed against amino acids 261–284, NCBI reference sequence NP_001074857.1); anti-GFP (mouse monoclonal; 4 μl of 0.4 μg/μl; Roche); peroxidase-conjugated secondary antibodies: anti-rabbit (goat polyclonal; 1:20,000; Sigma-Aldrich); anti-mouse (goat polyclonal; 1:5000; Sigma-Aldrich); GST (glutathione-S-transferase) pull-down: anti-GAPDH (rabbit monoclonal; 1:1000; Cell Signaling Technology); anti-HA (mouse monoclonal κ16B12; 1:1000; Biozym); affinity-purified anti-Cav1.3α1_2022–2138 (rabbit polyclonal; 1:1000; [51 (link)]). Peroxidase-conjugated secondary antibodies: anti-mouse (polyclonal goat; 1:3000; Roth) and anti-rabbit (polyclonal goat; 1:3000; Roth).

Commercially available antibodies were as follows: Alexa Fluor 555 goat anti-rabbit (Invitrogen, San Diego, CA; cat. # A21429 RRID:AB_141761), Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific, Waltham, MA; cat. # A11034 RRID:AB_10562715), Alexa Fluor 555 goat anti-rat (Invitrogen; cat. # A21434 RRID:AB_141733), Alexa Fluor 568 goat anti-mouse IgG2a (γ2a; Innovative Research of America, Toledo, OH; cat. # A21134 RRID:AB_1500825), Alexa Fluor 488 goat anti-mouse IgG1 (γ1; Invitrogen; cat. # A21121 RRID:AB_141514), anti-calretinin (Millipore, Bedford, MA; cat. # MAB1568 RRID:AB_94259), anti-synaptotagmin 2 (Zebrafish International Resource Center, Eugene, OR; cat. # znp-1 RRID:AB_10013783), anti-Ctbp2/ribeye (BD Biosciences, Franklin Lakes, NJ; cat. # 612044 RRID:AB_399431), anti-NK3R (rabbit polyclonal, gift of Dr. Arlene Hirano, University of California–Los Angeles, Los Angeles), anti–green fluorescent protein (GFP; Abcam, Cambridge, UK; cat. # ab13970 RRID:AB_300798), anti-calsenilin (Millipore; cat. # 05-756 RRID:AB_309969), and anti-CaBP1, anti-CaBP2, and anti-CaBP5 (Haeseleer et al., 2000 (link)).