The pre-DRE urine samples and 14 patient matched pre-/post-DRE samples were analyzed using DIA-MS on an Orbitrap Exploris™ 480 MS (Thermo Fisher Scientific™) connected to an EASY-nLC 1200 system nano-flow UHPLC (Thermo Fisher Scientific™). PicoFrit® LC-MS Columns (New Objective, MA, USA) were packed at 28 cm long with 1.9 μm/120Å ReproSil-Pur C18 resin (Dr. Maisch, Ammerbuch, Germany) for reversed phase peptide analyses. The N-linked glycosite-containing peptides were separated over a running time of 85 min using a gradient of 6–30 % buffer B (90 % acetonitrile and 0.1 % formic acid, buffer A with 3% acetonitrile and 0.1 % formic acid) at a flow rate of 200 nL/min. The MS parameters were as follows: MS1, AGC target 1 × 106, resolution 120,000, maximum injection time 60 ms, isolation window 12.0 m/z, scan range 400−1200 m/z; MS2, AGC target 1 × 106, resolution 30,000, maximum injection time 50 ms, and collision energy (%) 30. [24 (link),28 (link),29 (link)].
Picofrit lc ms columns
Manufactured by New Objective
Sourced in United States
The PicoFrit® LC-MS Columns are designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. They feature integrated emitter tips to facilitate direct coupling with mass spectrometers. The columns are available in various inner diameters and lengths to suit diverse analytical needs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using picofrit lc ms columns
1
Comprehensive Glycoproteomic Analysis of Urine Samples
2
Proteomics Workflow for Peptide Separation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Samples containing 50 ng peptides were separated by the EASY-nLCTM 1200 instrument of nano-flow UHPLC (Thermo Fisher Scientific™). Peptides were analyzed by reversed phase PicoFrit® LC–MS Columns (New Objective, MA, USA), which were packed with 0.9 μm/120 Å ReproSil-Pur C18 resin (Dr. Maisch, Ammerbuch, Germany) at 28 cm long. Global peptides were separated over a running time of 30 min using a 6–30% gradient of buffer B (90% acetonitrile and 0.1% formic acid) at a flow rate of 200 nL/min. The MS parameters were as follows: MS1, AGC target 1 × 106, resolution 120,000, maximum injection time 60 ms, isolation window 12.0 m/z, window overlap 2.0 m/z, scan range 400 − 1000 m/z; MS2, AGC target 1 × 106, resolution 15,000, maximum injection time 50 ms, and 31% collision energy.
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