Anti alpha tubulin 11224 1 ap

TA was purchased from Selleckchem (Houston, TX, USA). Antibodies in the present study were derived from the following sources: anti-beclin1 (11306-1-AP), anti-ATG7 (10088-2-AP), anti-acetyl Tubulin (Lys40) (66200-1-Ig), anti-Alpha Tubulin (11224-1-AP), and anti-Cyclophilin B (11607-1-AP), and all secondary antibodies for immunoblot analysis were purchased from Proteintech (RoSDont, IL). Anti-p62 (ab24182, Abcam) was from Abcam (Cambridge, UK). Anti-LC3 (NB100-2220) was obtained from Novus Biologicals. Anti-HDAC6 (7612) and anti-cleaved caspase 3 (9664, CST) were purchased from Cell Signaling Technology (Danvers, MA, USA). Cisplatin was obtained from Sigma (St. Louis, MO, USA).

Immunoblots were performed based on methods described in a previous paper [13 (link)]. The following antibodies were used: anti-NRF2 (GTX103322, GeneTex, Irvine, CA, USA), anti-GAPDH (60004-1g, Proteintech, Rosemont, IL, USA), anti-IκB alpha (12045-R116, Sino Biological, BDA, Beijing, China), anti-phospho- IκB alpha (#9246, Cell Signaling Technology, MA, USA), anti-alpha Tubulin (11224-1-AP, Proteintech, Rosemont, IL, USA), and anti-Lamin B1 (66095-1-Ig, Proteintech, Rosemont, IL, USA). Nuclear and cytoplasmic proteins were collected using EPIXTRACT Nuclear Protein Isolation Kit according to the manufacturer’s instructions.