Anti ter119 fitc

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-Ter119 FITC is a fluorescently-labeled monoclonal antibody that specifically binds to the Ter119 antigen expressed on mouse erythroid cells. It is used for the identification and quantification of erythroid cells in flow cytometry applications.

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4 protocols using anti ter119 fitc

Isolation and Characterization of Adipocyte Precursor Cells

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Epididymal and inguinal fat pads were cut in small pieces and incubated with 1 mg/ml collagenase I for 40 min^{44 (link),45 (link)}. The cell suspension was filtered through a 150 μm nylon mesh, and the stromalvascular fraction (SVF) was isolated by low-speed centrifugation. For FACS analysis, erythrocyte-free SVF cells were incubated with a mix of antibodies against different surface markers as described previously^{46 (link)} (anti-Ter119 FITC, eBioscience, 11-5921, 0.5 mg.ml⁻¹; anti-Cd45, FITC, eBioscience, 11-0451, 0.5 mg.ml⁻¹; anti-Cd31 FITC, eBioscience, 11-0311, 0.5 mg.ml⁻¹; anti-Sca1 PE/Cy7, BioLegend / Biozol, 122514, 1/500; and anti-Cd34 PE, abcam, ab23830, 1/250) and sorted using an Aria flow cytometer (BD Biosciences). Dead cells were removed using DAPI staining (1/10000). Cells negative for Ter119, Cd45, and Cd31 and positive for both Sca1 and Cd34 were considered as adipocyte precursor cells. Primary adipocytes were cultured in DMEM/F12 containing 10% foetal bovine serum (FBS).

Duteil D., Metzger E., Willmann D., Karagianni P., Friedrichs N., Greschik H., Günther T., Buettner R., Talianidis I., Metzger D, & Schüle R. (2014). LSD1 promotes oxidative metabolism of white adipose tissue. Nature communications, 5, 4093.

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Comprehensive Immune Cell Phenotyping

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Cells were incubated on ice for 45 min in Fc block in the presence of relevant primary antibodies. The anti-CD11c FITC, anti-CD11b PE, anti-CD103 PECy5, anti-MHCII APC-efluor780, anti-F4/80 efluor450, anti-CD45 BV510, anti-CX3CR1 PECy7, anti-Sca1 PE, anti-CD16/32 PerCP, anti-CD34 efluor450, anti-B220 FITC, anti-Gr1 FITC, anti-Ter119 FITC, anti-CD3 FITC, and anti-CD127 APC were purchased from eBioscience. The anti-CD11b PE, anti-CD11c APC, anti-Ly6C FITC, anti-CD117 PECy7 and anti-CD43 PerCP were purchased from BD Bioscience. After staining, cells were analyzed by flow cytometry on a FACS Canto. Data were analyzed with the FlowJo software.

Ibáñez L., Pontier-Bres R., Larbret F., Rekima A., Verhasselt V., Blin-Wakkach C, & Czerucka D. (2019). Saccharomyces boulardii Strain CNCM I-745 Modifies the Mononuclear Phagocytes Response in the Small Intestine of Mice Following Salmonella Typhimurium Infection. Frontiers in Immunology, 10, 643.

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Multiparametric Immunophenotyping of Immune Cells

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The anti-mouse antibodies, anti-TER119-FITC, anti-CD4 FITC, anti-CD24-PE, anti-CD44-PE-Cy7, and anti-CD25-APCCy7, were purchased from eBioscience (San Diego, CA, USA). Anti-CD8-FITC, anti-CD8-AF647, and anti-CD4-Pacific Blue were purchased from BD Biosciences (San Jose, CA, USA). The anti-human antibodies, anti-CD1a-PerCP-Cy5.5, anti-CD1a-PECy5, anti-CD3-APCCy7, anti-CD4-Pacific Blue, anti-CD7-FITC, anti-CD8-BV785, Anti-CD8-FITC, anti-CD34-PE, anti-CD34-PECy7, and anti-CD38-AF700 were purchased from Biolegend (San Diego, CA). Anti-Aiolos-PE, anti-Eos-PE, and anti-Helios-AF647 were purchased from e-Bioscience. Anti-Mouse IgG1κ-PE and anti-Ikaros-PE were purchased from BD Biosciences. The anti-Armenian Hamster IgG-AF647 control was purchased from Biolegend.

Mitchell J.L., Seng A, & Yankee T.M. (2017). Expression and splicing of Ikaros family members in murine and human thymocytes. Molecular immunology, 87, 1-11.

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Identification and Characterization of BM-MSCs

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Cells were resuspended in 100 μL of staining medium, followed by staining with fluorochrome-conjugated antibodies on ice for 20 minutes. The antibodies used in this study to identify MSCs were anti-CD45-FITC (eBioscience, clone 30-F11, 0.5 µg/test), anti-Ter119-FITC (eBioscience, clone TER-119, 0.25 µg/test), anti-CD31-FITC (eBioscience, clone 390, 1 µg/test), and anti-CD56-PE (R&D Systems, clone # 809220, 0.5 µg/test). Cells were analyzed on a Sony MA900 Cell Sorter, where CD45/Ter119/CD31^- cells were identified as BM-MSCs, and CD56 was used to separate CD56⁺ and CD56^- cell subtypes.

Wang Z., Li X., Yang J., Gong Y., Zhang H., Qiu X., Liu Y., Zhou C., Chen Y., Greenbaum J., Cheng L., Hu Y., Xie J., Yang X., Li Y., Schiller M.R., Chen Y., Tan L., Tang S.Y., Shen H., Xiao H.M, & Deng H.W. (2021). Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells. International Journal of Biological Sciences, 17(15), 4192-4206.

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