The sequences of the oligonucleotides were listed in Supplementary Table S1 . HPLC-purified fluorescent labeled primers, biotinylated extension oligonucleotides, and splint oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). Single-stranded oligonucleotides with ICL precursors, 39mer 20-bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6)-ICL), and 39mer 6-bp ICL (39+6-ICL) were synthesized and purified as previously reported (37 (link)). The identity of the ICLs was confirmed by LC–MS (Supplementary Figure S1A ).
Biotinylated extension oligonucleotides
Manufactured by Integrated DNA Technologies
Sourced in United States
Biotinylated extension oligonucleotides are single-stranded DNA molecules that have a biotin molecule attached to one end. The primary function of these oligonucleotides is to serve as probes or primers in various molecular biology applications, such as nucleic acid detection, labeling, and purification.
Automatically generated - may contain errors
2 protocols using biotinylated extension oligonucleotides
1
Synthesis and Characterization of DNA Interstrand Crosslinks
2
Synthesis and Characterization of ICL Oligonucleotides
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The sequences of the oligonucleotides were listed in Table S1. HPLC-purified fluorescent labeled primers, biotinylated extension oligonucleotides, and splint oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). Single-stranded oligonucleotides with ICL precursors, 39mer 8 atom 20 bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6) DMEDA ICL), and 39mer 8 atom 6 bp ICL (39+6 DMEDA ICL) were synthesized and purified as previously reported (33) . The identity of the ICLs was confirmed by
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