The single silk fiber was reeled from the cocoons in hot 0.5% (w/v) NaHCO3 solution. First, 50 mm of the single fiber was collected and cut into two fragments of lengths 20 and 30 mm, respectively. The 20 mm segment was used for diameter measurement, the 30 mm segment for the mechanical testing, respectively. The 20 mm segments were sputter coated in MP-19020NCTR Neocoater (JEOL, Tokyo, Japan) and then transferred to the JCM-5000 scanning electron microscope (JEOL) for diameter measurement. The single fiber with two brins was approximated as two small circles. Then, the diameters were used to calculate the cross-sectional areas of the small circles as the cross-sectional areas of single fiber. The diameter was used to calculate the cross-sectional areas of the single fiber. The initial length of the single fiber was measured using a caliper before mechanical testing. Tensile test was carried out using the single fiber on an AG-X plus instrument (Shimadzu, Kyoto, Japan) with a stretch speed of 2 mm/min. Each group measured 40 fibers for statistical analysis. The strain-stress curves were measured according to the method previously reported [18 (link)].
Neocoater mp 19020nctr
Manufactured by JEOL
Sourced in Japan, Australia
The NeoCoater MP-19020NCTR is a lab equipment product from JEOL. It is a coating machine designed for the deposition of thin films onto samples for various analytical and research applications. The core function of the NeoCoater MP-19020NCTR is to provide a controlled environment for the deposition of thin films, such as carbon, metals, or other materials, onto sample surfaces.
Automatically generated - may contain errors
Lab products found in correlation
Glass cover slip, by ProSciTech (6 mentions)
Fesem supra 40vp, by Zeiss (4 mentions)
Glass cover slips, by JEOL (3 mentions)
Supra 40vp, by Zeiss (3 mentions)
Neoscope jcm 5000, by JEOL (2 mentions)
Ag x plus instrument, by Shimadzu (1 mentions)
Jsm 6701, by JEOL (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Jsm 5610lv, by JEOL (1 mentions)
Jcm 6000, by JEOL (1 mentions)
Jsm 5000, by JEOL (1 mentions)
15 protocols using neocoater mp 19020nctr
1
Mechanical Properties of Silk Fibers
2
Platinum-Coated Scaffold Morphology
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The scaffolds were sprayed with Pt by a MP-19020NCTR NeoCoater (JEOL Ltd., Tokyo), and the morphology of the resultant scaffolds was observed with a scanning electron microscope (SEM) (JSM-6701; JEOL, Tokyo, JAPAN) at voltage of 25 kV. The mean fibre diameters were estimated using image analysis software (Image-Pro Plus, Rockville, MD, USA) by selecting 100 fibres randomly observed on the SEM images.
3
Nanoscale Analysis of Damselfly Wing Surface
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High-resolution SEM images of native wing surfaces, fungal conidia, and wing surfaces with attached conidia were obtained at 20,000, 60,000, and 100,000 magnifications using the SEM capabilities of the electron beam lithography tool (RAITH150 Two, Raith GmBH, Germany) at 5 kV. Wing samples were coated with thin gold films of 7.5 nm in thickness using a MP-19020NCTR NeoCoater (JEOL Ltd, Japan), before viewing with the microscope. The height of nanopillars was determined by tilting the samples at a 45°angle.
Nanopillar width and tip diameters were measured manually using ImageJ software (version 1.50i), an automated approach was found to be unsuitable because of the natural bending and clustering of the nanopillars. To obtain pillar density measurements using ImageJ, the colour threshold for binary SEM images (Figure S4) was adjusted and the particles were analysed. Particles with a diameter below 30 nm were removed as artefacts. The nanofeature density, inclusive of free-standing and clustering nanopillars, was determined using measurements taken over six regions, 1.5 lm  2 lm in area, on each of the wing surfaces of the damselfly.
Nanopillar width and tip diameters were measured manually using ImageJ software (version 1.50i), an automated approach was found to be unsuitable because of the natural bending and clustering of the nanopillars. To obtain pillar density measurements using ImageJ, the colour threshold for binary SEM images (Figure S4) was adjusted and the particles were analysed. Particles with a diameter below 30 nm were removed as artefacts. The nanofeature density, inclusive of free-standing and clustering nanopillars, was determined using measurements taken over six regions, 1.5 lm  2 lm in area, on each of the wing surfaces of the damselfly.
4
Visualizing SASD Powder Morphology
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Surface morphology of SASD in the powder form were visualized using a JCM-5000 NeoScope® microscope (JEOL, Akishima-Shi, Tokyo, Japan), at an accelerated voltage between 10 and 15 kV. Powder samples were stuck on a SEM stub with 146 conductive adhesive tapes and coated with gold to reduce electric charges, which is induced during analysis with a NeoCoater MP-19020NCTR® (JEOL, Akishima-Shi, Tokyo, Japan).
5
SEM Imaging of EMF-Exposed PC 12 Cells
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The scanning electron microscope FeSEM SUPRA 40VP (Carl Zeiss, Jena, Germany) with a primary beam energy of 3 kV was used. A 100 µl aliquot of cells in PBS was placed on a glass coverslip (ProSciTech, Kirwan, Australia) in duplicate. The glass coverslips were then washed with nanopure H2O (resistivity of 18.2 MW cm−1) and dried with 99.99% purity nitrogen gas. The PC 12 cells exposed to EMF of 18 GHz were fixed in a mixture of 2.0% paraformaldehyde and 2.5% glutaraldehyde for 30 min. The cells were then dehydrated by passing through a graded ethanol series (20%, 40%, 60%, 80% and 100%) for 15 min. Before imaging, the fixed cells were subjected to gold sputtering (7 nm thick) using a NeoCoater MP-19020NCTR (JEOL, Tokyo, Japan). The same procedure was applied to controls, non-exposed PC 12 cells.
6
SEM Imaging of THz-Exposed PC12 Cells
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The scanning electron microscope FeSEM SUPRA 40VP (Carl Zeiss, Jena, Germany) with a primary beam energy of 3 kV was used. A 100 µL aliquot of cells in PBS were placed on a glass cover slip (ProSciTech, Kirwan, Australia) in duplicate. The glass cover slips were then washed with nanopure H2O (resistivity of 18.2 MW cm−1) and dried with 99.99% purity nitrogen gas. The PC 12 cells exposed to 10 min of SS THz radiation were fixed in a cocktail of 2.0% paraformaldehyde and 2.5% glutaraldehyde for 30 min. The cells were then dehydrated by passing through a graded ethanol series (20%, 40%, 60%, 80% and 100%) for 15 min. Before imaging, the fixed cells were subjected to gold sputtering (7 nm thick) using a NeoCoater MP-19020NCTR (Jeol, Tokyo, Japan). The same procedure was applied to non-treated PC 12 cells.
7
Scanning Electron Microscopy of Red Blood Cells
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A field emission scanning electron microscope FeSEM – SUPRA 40VP (Carl Zeiss, Jena, Germany), with a primary beam energy of 3 kV, was used to obtain high-resolution images of the cell samples. A 100 µL aliquot of each sample was placed on a glass cover slip (ProSciTech, Kirwan, Australia), in duplicate, and allowed to sit for 10 min. All samples were then fixed in 2.5% glutaraldehyde (Sigma) for 30 min and progressively dehydrated using graded ethanol solutions (30, 50, 70, 90, and 100% v/v) for 10 min. The glass cover slips were air-dried and then subjected to gold sputtering (6 nm thick gold film) using a NeoCoater MP-19020NCTR (JEOL, Frenchs Forest, Australia) instrument. Approximately thirty SEM images for each experimental step were obtained at a magnification of 3,000× for subsequent statistical analysis. For each SEM image, the number of morphologically unchanged RBCs was manually counted.
8
Membrane Morphology Analysis by SEM
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Scanning electron microscopy (SEM) was employed to investigate the morphology of the membrane. The SEM images were recorded using a NeoScope JCM5000 (JEOL, Tokyo, Japan) with a 10 kV electron beam. The membranes were gold coated using a NeoCoater MP-19020NCTR (JEOL, Tokyo, Japan) prior to the observations.
9
SEM Imaging of Lyophilized Samples
10
Cell Morphology Evaluation via SEM
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Scanning electron microscopy (SEM) was used to assess the cell morphology following incubation on AR and HTE Ti surfaces. Prior to SEM analysis, the cells were fixed with 2.5% glutaraldehyde for 25 min. The cells were then dehydrated by passing through a 30%, 50%, 70%, and 100% graded ethanol series for 15 min each. Before imaging, samples were gold sputtered using a NeoCoater MP-19020NCTR (JEOL, Tokyo, Japan). SEM images were taken using a field emission SEM (FESEM) SUPRA 40VP (Carl Zeiss, Jena, Germany) at an accelerating voltage of 3 kV at magnifications of 10,000× for AR and 2000× for HTE samples.
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