P erbb3 tyr1289

Antibodies against ERBB2 (#2242), phosphorylated (p-)ERBB2 (Tyr1248) (#2247), ERBB3 (C-terminus) (#12708), p-ERBB3 (Tyr1289) (#4791), ERK (#4695), p-ERK (#4370), AKT (#4685), p-AKT (#4060), GFP (#2555), Myc (#2272), and GAPDH (#5174) were purchased from Cell Signaling Technology.

TSA, PXD101 and erlotinib were purchased from Selleckchem (Houston, TX). MG132 and recombinant human EGF were purchased from Sigma-Aldrich (St Louis, MO) and ProSpecBio (East Brunswick, NJ), respectively. The Arf1 dominant negative and constitutively active constructs pcDNA3-HA-Arf1 DN-T31 N (Arf1DN) and pcDNA3-HA-Arf1-ActQ71L (Arf1CA) were purchased from Addgene (Plasmid #10833 and #10832). Antibodies that recognize acetyl-Histone H3 (Lys9/Lys14), acetyl-Histone H4 (Lys8), p-AKT (Ser473), AKT, p-ERK1/2 (Thr202/Tyr204), ERK1/2, p-STAT3 (Tyr705), STAT3, p-Src (Tyr416), Src, p-EGFR (Tyr845), EGFR, p-ErbB2 (Tyr1221/1222), ErbB2, p-ErbB3 (Tyr1289) and ErbB3, were purchased from Cell Signaling Technology (Beverly, MA). β-actin and PY20 antibodies were purchased from Sigma-Aldrich (St Louis, MO). CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Kit was obtained from Promega (Madison, MI).

BPAF was purchased from Sigma (St. Louis, MO). LY294002 (PI3K inhibitor) and Iressa (Gefitinib) were purchased from LC Laboratories (Woburn, MA). Primary antibodies against ERα, phosphorylated-ERα (p-ERα) (Ser106), ERβ, ERRγ, Akt, Erk2, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). p-ERα (Ser118), Cyclin D1, c-Myc, ErbB3, p-ErbB3 (Tyr1289), p-Akt (Ser473), p-Erk1/2 (Thr202/Tyr204), and p-Src (Tyr416) primary antibodies were purchased from Cell Signaling (Danvers, MA). The primary antibody against p-ERα (Ser167) was purchased from Thermo Fisher Scientific (Rockford, IL).