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Anti mouse cd90.1 clone ox 7

Manufactured by BioLegend
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Anti-mouse CD90.1 (Clone OX-7) is a monoclonal antibody that recognizes the CD90.1 (Thy-1.1) cell surface antigen expressed on a variety of mouse cells, including T cells, thymocytes, and stem cells.

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Lab products found in correlation

Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Anti mouse cd8 clone 53 6, by BioLegend (1 mentions) Anti mouse cd45.1 clone a20, by BioLegend (1 mentions) Anti mouse cd4 clone gk1, by BioLegend (1 mentions) Anti mouse tcrβ, by BioLegend (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg f ab 2, by Jackson ImmunoResearch (1 mentions) Goat anti human igg f ab 2, by Jackson ImmunoResearch (1 mentions) Goat anti mouse igg 680, by LI COR (1 mentions)

2 protocols using anti mouse cd90.1 clone ox 7

1

Multicolor Immunofluorescence Staining of Murine Tissues

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Mouse spleens or kidneys were embedded in OCT tissue media (Tissue-Tek) and frozen on dry ice. Frozen sections (7-μm thickness) were fixed to slides in ice-cold acetone for 15 min and air dried for 30 s. The sections were blocked with 2% BSA for 30 min at room temperature and then stained for 30 min at room temperature in a humidified chamber with fluorescently labeled antibody cocktails and Hoechst 33258 (Life Technologies). The following fluorescence antibodies were applied according to the manufacturer’s instructions: anti-mouse MARCO (BIO-RAD), anti-mouse CD45.1 (Clone A20,Biolegend), anti-mouse CD4 (Clone GK1.5, Biolegend), anti-mouse CD8 (Clone 53–6.7, Biolegend), anti-mouse CD90.1 (Clone OX-7, Biolegend), anti-mouse TCRβ (Clone B20.6, Biolegend), goat anti-mouse IgG cross-adsorbed secondary antibody (ThermoFisher Scientific). All tissue sections were mounted in Prolong Gold Antifade Mountant (Thermo Fisher Scientific) and viewed with Zeiss LSM510 Upright Confocal System.
Li H., Adamopoulos I.E., Moulton V.R., Stillman I.E., Herbert Z., Moon J.J., Sharabi A., Krishfield S., Tsokos M.G, & Tsokos G.C. (2020). Systemic lupus erythematosus favors the generation of IL-17 producing double negative T cells. Nature Communications, 11, 2859.
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2

Antibody Characterization for Cell Analysis

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Anti-CD98 clone UM7F8, mIgG1 control antibody clone MOPC21, anti-mouse cKit clone ACK45 and anti-human NGFR clone C40–1457 were purchased from BD. Anti-Mouse CD90.1 Clone OX-7, anti-CD98 clone MEM108, and anti-mouse CD98 Clone RL388, were purchased from Biolegend. Anti-CD98 H300 was purchased from Santa Cruz Biotechnology. Anti-Ubiquitin clone P4D1 and anti-Met clone L41G3 were purchased from CST. Goat Anti-human IgG F(ab’)2, Goat Anti-mouse IgG F(ab’)2, and Goat anti-mouse IgG F(ab’) were purchased from Jackson. Humanized anti-human MET (LY2875358/Emibetuzumab) and non-cross competing MET antibody (LSN2148068) have been described previously and were provided by Eli Lilly(13 (link)). LSN2148068 was conjugated to DyLight650 using a DyLight microscale conjugation kit (Life Technologies) according to the manufacturer’s directions. Secondary reagents for western blot (Goat anti-mouse IgG-800, Goat anti-mouse IgG-680, Goat anti-rabbit IgG-800 and goat anti-rabbit IgG-680 were obtained from Licor.
Ablack J.N., Ortiz J., Bajaj J., Trinh K., Lagarrigue F., Cantor J.M., Reya T, & Ginsberg M.H. (2020). MARCH Proteins Mediate Responses to Anti-tumor Antibodies. Journal of immunology (Baltimore, Md. : 1950), 205(10), 2883-2892.
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