Mono methyl arginine r gg d5a12

Manufactured by Cell Signaling Technology

Mono-Methyl Arginine (R*GG; D5A12) is a reagent used in biochemical research applications. It is a post-translational modification of the amino acid arginine, where a single methyl group is attached. This modification can be involved in various cellular signaling pathways and processes. The specific function and intended use of this product should be determined by the researcher based on their experimental design and needs.

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Lab products found in correlation

Gapdh 14c10, by Cell Signaling Technology (1 mentions) Pabp1, by Cell Signaling Technology (1 mentions) 4 hydroxytamoxifen h7904, by Merck Group (1 mentions) Phospho eif4e ser209, by Cell Signaling Technology (1 mentions) Eif4g1, by Cell Signaling Technology (1 mentions) Eif4e c46h6, by Cell Signaling Technology (1 mentions) Phospho eif2alpha ser51, by Cell Signaling Technology (1 mentions) Ha tag 6e2, by Cell Signaling Technology (1 mentions) Anti gfp ab6556, by Abcam (1 mentions) Hdac1, by Abcam (1 mentions) Ddx17, by Abcam (1 mentions) Ha 11, by BioLegend (1 mentions) Lamin a c, by Abcam (1 mentions) Sym10, by Merck Group (1 mentions) H4r3me2a, by Active Motif (1 mentions) Taf15, by Fortis Life Sciences (1 mentions) Drosha, by Santa Cruz Biotechnology (1 mentions) Prmt1, by Abcam (1 mentions) Asym24, by Merck Group (1 mentions) Dgcr8, by Abcam (1 mentions)

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Mono-Methyl Arginine (R*GG)]. (2 citations) Mono-methyl arginine (2 citations)

2 protocols using mono methyl arginine r gg d5a12

Antibody Resource for Cell Signaling

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The following antibodies were obtained from Cell Signaling Technology: Mono-Methyl Arginine (R*GG; D5A12; #8711), Mono-Methyl Arginine (Me-R4-100; #8015), eIF4G1 (#2858), eIF4G1 (D6A6; #8701), eIF4E (C46H6; #2067), Phospho-eIF4E (Ser209; #9741), eIF4A (C32B4; #2013), eIF2alpha (D7D3; #5324), phospho-eIF2alpha (Ser51; #3398), PABP1 (#4992), GAPDH (14C10; #2118), HA-Tag (6E2; HRP Conjugate), mouse anti-rabbit IgG conformation specific (#5127), and rabbit IgG isotype control (#3900). We purchased Prmt1 antibodies from Millipore (#07-404) and Abcam (ab73246 and ab7027). p53 (#554147) and p21 (sc-6246) antibody were purchased from BD Pharmingen and Sana Cruz Biotechnology, Inc., respectively. Anti-GFP (ab6556) was obtained from Abcam. Anti-FLAG M2 antibody (F1804), Anti-FLAG M2 affinity gel (A2220), doxycycline (D9891) and 4-hydroxytamoxifen (H7904) were purchased from Sigma. We obtained cycloheximide (#239763) and G418 from Calbiochem and Corning, respectively.

Hsu J.H., Hubbell-Engler B., Adelmant G., Huang J., Joyce C.E., Vazquez F., Weir B.A., Montgomery P., Tsherniak A., Giacomelli A.O., Perry J.A., Trowbridge J., Fujiwara Y., S.Cowley G., Xie H., Kim W., Novina C.D., Hahn W.C., Marto J.A, & Orkin S.H. (2017). Prmt1-mediated translation regulation is a crucial vulnerability of cancer. Cancer research, 77(17), 4613-4625.

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Protein Immunoprecipitation and Western Blot

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The following antibodies were used for immuno-precipitation and Western Blot experiments, according to the manufacturer's instruction: DGCR8 (Abcam ab90579), EWSR1 (Abcam ab54708), FUS (Bethyl A300–293A), DDX5 (Abcam ab126730), Drosha (Santa Cruz sc-33778), Vinculin (VCL) (Millipore 06–866), PRMT1 (Abcam ab73246), ASYM24 (Millipore), SYM10 (Millipore), Mono-Methyl Arginine (R*GG) (D5A12) (Cell Signaling Technology 8711), LAMIN A/C (sc-6215), Lamin B1 (Abcam ab16048), GAPDH (Abcam ab9484), H3 (Abcam ab1791), H4 (Abcam ab7311), H4R3me2a (Active Motif 39705), TAF15 (Bethyl Laboratories A300–308A), ILF3 (Bethyl A303–615A), ILF2 (sc-271718), DDX17 (sc-130650), HDAC1 (Abcam ab7028) and HA.11 (Biolegend 901513).
MS023 and MS094 compounds were kindly provided by the SGC Toronto—Structural Genomic Consortium (http://www.thesgc.org/scientists/groups/toronto). Compounds were dissolved in DMSO and used at a final concentration of 10 μM for the indicated time intervals.

Spadotto V., Giambruno R., Massignani E., Mihailovich M., Maniaci M., Patuzzo F., Ghini F., Nicassio F, & Bonaldi T. (2019). PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis. Nucleic Acids Research, 48(1), 96-115.

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