Lobind microtubes

Manufactured by Eppendorf

Sourced in Germany

LoBind microtubes are specialized laboratory microcentrifuge tubes designed to minimize sample loss due to non-specific binding. These tubes feature a low-binding surface treatment that helps to prevent the adsorption of proteins, nucleic acids, and other biomolecules to the tube walls, ensuring improved sample recovery and preservation.

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Lab products found in correlation

Innotest sandwich elisa, by Fujirebio (1 mentions) Polypropylene tube, by Sarstedt (1 mentions) Lysis buffer, by Thermo Fisher Scientific (1 mentions) Dynabeads mrna direct purification kit, by Thermo Fisher Scientific (1 mentions) Nexseq 500, by Illumina (1 mentions)

2 protocols using lobind microtubes

Standardized CSF Collection and Analysis Protocols

Cited 2 times

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CSF from individuals with AD and AMC in the WashU‐A cohort were collected via a catheter as previously described.²¹ (link) CSF from AMC and individuals with symptomatic AD, PSP, CBS, and bvFTD in the WashU‐B cohort were obtained via LP with gravity collection and centrifugation as previously described.²² (link) CSF from MAPT mutation families was collected according to the standardized protocol at the Biomarker Core at the Washington University School of Medicine.³² CSF from individuals with brain tumors was obtained via lumbar drain using a catheter before or after surgery.
CSF from the Montpellier cohort was collected using the standardized protocol for the collection, centrifugation, and storage at Memory Resources and Research Center of Montpellier.³³ (link), ³⁴ (link), ³⁵ (link) Briefly, the atraumatic needle was used for LP, with CSF collected into 10 mL polypropylene tube (ref 62.610.201, Sarstedt, Germany) and protein low binding Eppendorf^® tubes (LoBind microtubes Eppendorf, ref 022431064, Hamburg, Germany). CSF was not centrifuged before aliquoting and storage at −80°C. CSF tau and pT181 concentrations were measured using the standardized commercially available INNOTEST sandwich ELISA X‐MAP following Fujirebio instructions. CSF Aβ 42 and Aβ 40 were measured using INNOTEST sandwich ELISA from Fujirebio.

Sato C., Mallipeddi N., Ghoshal N., Wright B.A., Day G.S., Davis A.A., Kim A.H., Zipfel G.J., Bateman R.J., Gabelle A, & Barthélemy N.R. (2021). MAPT R406W increases tau T217 phosphorylation in absence of amyloid pathology. Annals of Clinical and Translational Neurology, 8(9), 1817-1830.

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Bulk RNA-seq Analysis of Dendritic Cells

Cited 3 times

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For bulk RNA-seq analysis, 5,000 DC were facs-sorted into 1.7 ml LoBind microtubes (Eppendorf) containing 40 μl of lysis buffer (Life Technologies). RNA was captured with Dynabeads mRNA Direct Purification Kit (Life Technologies) according to the manufacturer's instructions. The RNA-seq protocol for the generation of libraries is a derivation of MARS-seq (36 (link)). RNA-seq libraries were sequenced using Illumina NexSeq-500, raw data were mapped to the genome (NCBI37/mm9) using HISAT (version 0.1.6) (57 (link)), only reads with unique mapping were considered. Gene expression levels were calculated using HOMER software package (analyzeRepeats.pl rna mm9 -d < tagDir> -count exons -condenseGenes -strand + -raw) (58 (link)). Normalization and differential expression analysis were done using the DESeq2 R-package (Bioconductor, https://bioconductor.org/packages/release/bioc/html/DESeq2.html) (59 (link)). Differentially expressed genes were selected using a 2-fold change and p-value < 0.05 (Figures 3B,D), or a 1.8-fold-change and p-value < 0.05 (Figures 4B,C) or p-value < 0.01(Figure 4D) between at least two conditions. Gene expression matrix was clustered using a k-means algorithm (Matlab function kmeans) with correlation as the distance metric. Heat maps were generated using Genee software.

Curato C., Bernshtein B., Zupancič E., Dufner A., Jaitin D., Giladi A., David E., Chappell-Maor L., Leshkowitz D., Knobeloch K.P., Amit I., Florindo H.F, & Jung S. (2019). DC Respond to Cognate T Cell Interaction in the Antigen-Challenged Lymph Node. Frontiers in Immunology, 10, 863.

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