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Recombinant rat igf 1

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Recombinant rat IGF-1 is a protein produced in a laboratory using recombinant DNA technology. It is the rat version of the Insulin-like Growth Factor 1 (IGF-1) protein, which is involved in cell growth and development processes.

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Lab products found in correlation

Roswell park memorial institute (rpmi), by R&D Systems (1 mentions) Charcoal stripped fbs, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Cell proliferation kit 1, by Roche (1 mentions) Igf 1, by R&D Systems (1 mentions) Lsm fv1000, by Olympus (1 mentions) Alzet osmotic minipump, by Durect (1 mentions)

Close spelling variants of this product

Recombinant IGF-1 IGF-1

4 protocols using recombinant rat igf 1

1

Evaluating IGF-1 and DHT Effects on Dunning G Cells

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Dunning G cells (5000 cells/well) were seeded in 100 µl phenol-free RPMI (Gibco) supplemented with 2.5% charcoal stripped FBS (Gibco), in 96-well plates, and allowed to settle overnight. Culture medium was carefully removed and replaced with 100 µl phenol-free RPMI supplemented with 2.5% charcoal stripped FBS and various concentrations of recombinant rat IGF-1 (R&D Systems, Minneapolis, MN, US), dihydrotestosterone (DHT, Sigma-Aldrich, St Louis, MO, US) and NVP-AEW541, at doses previously described [13 (link)]. The relative amount of viable tumor cells was measured at baseline, and day 2, 4 and 7 using the Cell Proliferation Kit I (Roche Diagnostics, Bromma, Sweden) according to the manufacturer’s instructions. Cells that were cultured for 7 days received fresh media with supplements at day 4 of the experiment.
Nordstrand A., Bergström S.H., Thysell E., Bovinder-Ylitalo E., Lerner U.H., Widmark A., Bergh A, & Wikström P. (2017). Inhibition of the insulin-like growth factor-1 receptor potentiates acute effects of castration in a rat model for prostate cancer growth in bone. Clinical & Experimental Metastasis, 34(3), 261-271.
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2

IGF-1 Effects on ICH-Induced Acute Brain Injury

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Recombinant rat IGF-1 (R&D system, Minneapolis, MN, U.S.A.; 10 μg/ml) and a vehicle (PBS) were administered via intraperitoneal injection 30 min after the ICH surgery to assess the effects of IGF-1 on the regulation of ICH-induced acute brain injury.
Yuan J.J., Zhang Q., Gong C.X., Wang F.X., Huang J.C., Yang G.Q., Liu L., Zhou K., Xu R., Chen Q., Zhou Y., Xiong X.Y, & Yang Q.W. (2019). Young plasma ameliorates aging-related acute brain injury after intracerebral hemorrhage. Bioscience Reports, 39(5), BSR20190537.
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3

Glucose Uptake in Neuronal Cells

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Neurons were plated on glass bottom culture dishes and were used at 6–8 DIV. For the experiments, cultures were rinsed with glucose-free HCSS (120 mmol/l NaCl, 5.4 mmol/l KCl, 1.8 mmol/l CaCl2, 20 mmol/l HEPES pH 7.4) and kept for 45 min under glucose deprivation followed by exposure for 30 min to either mAß1−42 (100 nM), or recombinant rat IGF-1 (5 ng/ml, R&D Systems). The non-hydrolyzable glucose analog 6-NBDG was allowed to be internalized into neuronal cells at 37°C and 5% CO2 for 10 min. 6-NBDG+ neurons were imaged by using an Olympus FV1000 LSM. Images were captured at 488 excitation/505-550 emission.
Giuffrida M.L., Tomasello M.F., Pandini G., Caraci F., Battaglia G., Busceti C., Di Pietro P., Pappalardo G., Attanasio F., Chiechio S., Bagnoli S., Nacmias B., Sorbi S., Vigneri R., Rizzarelli E., Nicoletti F, & Copani A. (2015). Monomeric ß-amyloid interacts with type-1 insulin-like growth factor receptors to provide energy supply to neurons. Frontiers in Cellular Neuroscience, 9, 297.
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4

IGF-1 Infusion and BrdU Labeling in Rats

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Recombinant rat IGF-1 was obtained from R&D Systems (Minneapolis, MN) diluted in saline and given at 4 μg/Kg/day as a continuous subcutaneous infusion using the Alzet osmotic mini-pumps (Model 2006, flow rate 1 μl/hr; Durect Co, Cupertino, CA). For the control group, equal volume of saline was loaded into osmotic pumps. Amount of IGF-1 was based on our pilot study and previous report [28 (link)]. Isofluorane was used for anesthetic induction and maintenance during surgical implantation of the pump subcutaneously (above the scapula) for each animal. Topical lidocaine was applied at the incision site after isofluorane induction for pain management. In addition, post-operative acetaminophen (200 mg/kg) was delivered in the drinking water 24 hours after pump implantation.
The thymidine analog Bromodeoxyuridine (Chemicon, Temecula, CA) was also given to label proliferating neural progenitor cells. Bromodeoxyuridine (BrdU) was dissolved in 0.9% sterile NaCl and filtered at 22 μm. The resulting solution was injected at 75 mg/kg intraperitoneally. BrdU injections were given for 3 consecutive days starting at two weeks after completion of the chemotherapeutic regimen.
Briones T.L., Woods J, & Wadowska M. (2014). Involvement of Insulin-like Growth Factor-1 in Chemotherapy-related Cognitive Impairment. Behavioural brain research, 279, 112-122.
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